Measuring beta‐galactose exposure on platelets: Standardization and healthy reference values
Abstract Background Correct diagnosis of the cause of thrombocytopenia is crucial for the appropriate management of patients. Hyposialylation/desialylation (characterized by abnormally high β‐galactose exposure) accelerates platelet clearance and can lead to thrombocytopenia. However, the reference...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Research and Practice in Thrombosis and Haemostasis |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/rth2.12369 |
| _version_ | 1797762092032327680 |
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| author | Dominique Lasne Tiffany Pascreau Sadyo Darame Marie‐Charlotte Bourrienne Peggy Tournoux Aurélien Philippe Sara Ziachahabi Felipe Suarez Ambroise Marcais Annabelle Dupont Cécile V. Denis Alexandre Kauskot Delphine Borgel |
| author_facet | Dominique Lasne Tiffany Pascreau Sadyo Darame Marie‐Charlotte Bourrienne Peggy Tournoux Aurélien Philippe Sara Ziachahabi Felipe Suarez Ambroise Marcais Annabelle Dupont Cécile V. Denis Alexandre Kauskot Delphine Borgel |
| author_sort | Dominique Lasne |
| collection | DOAJ |
| description | Abstract Background Correct diagnosis of the cause of thrombocytopenia is crucial for the appropriate management of patients. Hyposialylation/desialylation (characterized by abnormally high β‐galactose exposure) accelerates platelet clearance and can lead to thrombocytopenia. However, the reference range for β‐galactose exposure in healthy individuals has not been defined previously. Objective The objective of the present study was to develop a standardized assay of platelet β‐galactose exposure for implementation in a clinical laboratory. Methods β‐Galactose exposure was measured in platelet‐rich plasma by using flow cytometry and Ricinus communis agglutinin (RCA). A population of 120 healthy adults was recruited to study variability. Results We determined an optimal RCA concentration of 12.5 μg/mL. The measure was stable for up to 4 hours (mean fluorescence intensity [MFI]‐RCA: 1233 ± 329 at 0 hour and 1480 ± 410 at 4 hours). The platelet count did not induce a variation of RCA and the measure of RCA was stable when tested up to 24 hours after blood collection (MFI‐RCA: 1252 ± 434 at day 0 and 1140 ± 297 24 hours after blood sampling). To take into account the platelet size, results should be expressed as RCA/forward scatter ratio. We used the assay to study variability in 120 healthy adults, and we found that the ratio is independent of sex and blood group. Conclusion We defined a normal range in a healthy population and several preanalytical and analytical variables were evaluated, together with positive and negative controls. This assay may assist in the diagnosis of thrombocytopenic diseases linked to changes in β‐galactose exposure. |
| first_indexed | 2024-03-12T19:22:23Z |
| format | Article |
| id | doaj.art-fb8cc2610a0a4df0821dc427566bb3e6 |
| institution | Directory Open Access Journal |
| issn | 2475-0379 |
| language | English |
| last_indexed | 2024-03-12T19:22:23Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Research and Practice in Thrombosis and Haemostasis |
| spelling | doaj.art-fb8cc2610a0a4df0821dc427566bb3e62023-08-02T05:05:51ZengElsevierResearch and Practice in Thrombosis and Haemostasis2475-03792020-07-014581382210.1002/rth2.12369Measuring beta‐galactose exposure on platelets: Standardization and healthy reference valuesDominique Lasne0Tiffany Pascreau1Sadyo Darame2Marie‐Charlotte Bourrienne3Peggy Tournoux4Aurélien Philippe5Sara Ziachahabi6Felipe Suarez7Ambroise Marcais8Annabelle Dupont9Cécile V. Denis10Alexandre Kauskot11Delphine Borgel12Department of Biological Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Biological Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Biological Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Biological Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Biological Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Biological Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Biological Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Hematology Hôpital Necker AP‐HP Paris FranceDepartment of Haemostasis and Transfusion CHU Lille Lille FranceHITh UMR_S 1176 INSERM Univ. Paris‐Saclay Le Kremlin‐Bicêtre FranceHITh UMR_S 1176 INSERM Univ. Paris‐Saclay Le Kremlin‐Bicêtre FranceDepartment of Biological Hematology Hôpital Necker AP‐HP Paris FranceAbstract Background Correct diagnosis of the cause of thrombocytopenia is crucial for the appropriate management of patients. Hyposialylation/desialylation (characterized by abnormally high β‐galactose exposure) accelerates platelet clearance and can lead to thrombocytopenia. However, the reference range for β‐galactose exposure in healthy individuals has not been defined previously. Objective The objective of the present study was to develop a standardized assay of platelet β‐galactose exposure for implementation in a clinical laboratory. Methods β‐Galactose exposure was measured in platelet‐rich plasma by using flow cytometry and Ricinus communis agglutinin (RCA). A population of 120 healthy adults was recruited to study variability. Results We determined an optimal RCA concentration of 12.5 μg/mL. The measure was stable for up to 4 hours (mean fluorescence intensity [MFI]‐RCA: 1233 ± 329 at 0 hour and 1480 ± 410 at 4 hours). The platelet count did not induce a variation of RCA and the measure of RCA was stable when tested up to 24 hours after blood collection (MFI‐RCA: 1252 ± 434 at day 0 and 1140 ± 297 24 hours after blood sampling). To take into account the platelet size, results should be expressed as RCA/forward scatter ratio. We used the assay to study variability in 120 healthy adults, and we found that the ratio is independent of sex and blood group. Conclusion We defined a normal range in a healthy population and several preanalytical and analytical variables were evaluated, together with positive and negative controls. This assay may assist in the diagnosis of thrombocytopenic diseases linked to changes in β‐galactose exposure.https://doi.org/10.1002/rth2.12369blood plateletsgalactoseN‐acetylneuraminic acidplatelet countreferences values |
| spellingShingle | Dominique Lasne Tiffany Pascreau Sadyo Darame Marie‐Charlotte Bourrienne Peggy Tournoux Aurélien Philippe Sara Ziachahabi Felipe Suarez Ambroise Marcais Annabelle Dupont Cécile V. Denis Alexandre Kauskot Delphine Borgel Measuring beta‐galactose exposure on platelets: Standardization and healthy reference values Research and Practice in Thrombosis and Haemostasis blood platelets galactose N‐acetylneuraminic acid platelet count references values |
| title | Measuring beta‐galactose exposure on platelets: Standardization and healthy reference values |
| title_full | Measuring beta‐galactose exposure on platelets: Standardization and healthy reference values |
| title_fullStr | Measuring beta‐galactose exposure on platelets: Standardization and healthy reference values |
| title_full_unstemmed | Measuring beta‐galactose exposure on platelets: Standardization and healthy reference values |
| title_short | Measuring beta‐galactose exposure on platelets: Standardization and healthy reference values |
| title_sort | measuring beta galactose exposure on platelets standardization and healthy reference values |
| topic | blood platelets galactose N‐acetylneuraminic acid platelet count references values |
| url | https://doi.org/10.1002/rth2.12369 |
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