Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence
BackgroundPatients with alcohol dependence (AD) can exhibit gut dysbacteria. Dysbacteria may co-occur with disruptions of circadian rhythmicity of the gut flora, which can aggravate AD. Herein, this study aimed to investigate diurnal oscillations of the gut microbiota in AD patients.MethodsThirty-tw...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1127011/full |
| _version_ | 1797904051540590592 |
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| author | Kangqing Zhao Zhaojun Ni Ying Qin Ran Zhu Zhoulong Yu Yundong Ma Wenhao Chen Qiqing Sun Zhong Wang Yanjing Liu Jingwen Zhao Wenjuan Peng Sifan Hu Jie Shi Jie Shi Jie Shi Lin Lu Hongqiang Sun |
| author_facet | Kangqing Zhao Zhaojun Ni Ying Qin Ran Zhu Zhoulong Yu Yundong Ma Wenhao Chen Qiqing Sun Zhong Wang Yanjing Liu Jingwen Zhao Wenjuan Peng Sifan Hu Jie Shi Jie Shi Jie Shi Lin Lu Hongqiang Sun |
| author_sort | Kangqing Zhao |
| collection | DOAJ |
| description | BackgroundPatients with alcohol dependence (AD) can exhibit gut dysbacteria. Dysbacteria may co-occur with disruptions of circadian rhythmicity of the gut flora, which can aggravate AD. Herein, this study aimed to investigate diurnal oscillations of the gut microbiota in AD patients.MethodsThirty-two patients with AD, based on the Diagnostic and Statistical Manual of Mental Disorders, 4th edition, and 20 healthy subjects were enrolled in this study. Demographic and clinical data were collected by self-report questionnaires. Fecal samples at 7:00 AM, 11:00 AM, 3:00 PM, and 7:00 PM were collected from each subject. 16S rDNA sequencing was conducted. Wilcoxon and Kruskal-Wallis tests were performed to characterize alterations and oscillations of the gut microbiota.ResultsWe found that β-diversity of the gut microbiota in AD patients oscillated diurnally compared with healthy subjects (p = 0.01). Additionally, 0.66% of operational taxonomic units oscillated diurnally in AD patients versus 1.68% in healthy subjects. At different taxonomic levels, bacterial abundance oscillated diurnally in both groups, such as Pseudomonas and Prevotella pallens (all p < 0.05). β-diversity of the gut microbiota in AD patients with high daily alcohol consumption, high-level cravings, short AD durations, and mild withdrawal symptoms oscillated diurnally compared with other AD patients (all p < 0.05).ConclusionThe gut microbiota in AD patients exhibits disruptions of diurnal oscillation, which may provide novel insights into mechanisms of AD and the development of therapeutic strategies. |
| first_indexed | 2024-04-10T09:42:42Z |
| format | Article |
| id | doaj.art-fb8f754bdaaa42c392c39c23c41f7b8a |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-10T09:42:42Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-fb8f754bdaaa42c392c39c23c41f7b8a2023-02-17T06:54:41ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-02-011310.3389/fcimb.2023.11270111127011Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependenceKangqing Zhao0Zhaojun Ni1Ying Qin2Ran Zhu3Zhoulong Yu4Yundong Ma5Wenhao Chen6Qiqing Sun7Zhong Wang8Yanjing Liu9Jingwen Zhao10Wenjuan Peng11Sifan Hu12Jie Shi13Jie Shi14Jie Shi15Lin Lu16Hongqiang Sun17NHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaAddiction Medicine Department, The Second People’s Hospital of Guizhou Province, Guizhou, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaAddiction Medicine Department, The Second People’s Hospital of Guizhou Province, Guizhou, ChinaAddiction Medicine Department, The Second People’s Hospital of Guizhou Province, Guizhou, ChinaAddiction Medicine Department, The Second People’s Hospital of Guizhou Province, Guizhou, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaNational Institute on Drug Dependence and Beijing Key Laboratory of Drug Dependence, Peking University, Beijing, ChinaThe State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, ChinaThe Key Laboratory for Neuroscience of the Ministry of Education and Health, Peking University, Beijing, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaNHC Key Laboratory of Mental Health (Peking University), Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, ChinaBackgroundPatients with alcohol dependence (AD) can exhibit gut dysbacteria. Dysbacteria may co-occur with disruptions of circadian rhythmicity of the gut flora, which can aggravate AD. Herein, this study aimed to investigate diurnal oscillations of the gut microbiota in AD patients.MethodsThirty-two patients with AD, based on the Diagnostic and Statistical Manual of Mental Disorders, 4th edition, and 20 healthy subjects were enrolled in this study. Demographic and clinical data were collected by self-report questionnaires. Fecal samples at 7:00 AM, 11:00 AM, 3:00 PM, and 7:00 PM were collected from each subject. 16S rDNA sequencing was conducted. Wilcoxon and Kruskal-Wallis tests were performed to characterize alterations and oscillations of the gut microbiota.ResultsWe found that β-diversity of the gut microbiota in AD patients oscillated diurnally compared with healthy subjects (p = 0.01). Additionally, 0.66% of operational taxonomic units oscillated diurnally in AD patients versus 1.68% in healthy subjects. At different taxonomic levels, bacterial abundance oscillated diurnally in both groups, such as Pseudomonas and Prevotella pallens (all p < 0.05). β-diversity of the gut microbiota in AD patients with high daily alcohol consumption, high-level cravings, short AD durations, and mild withdrawal symptoms oscillated diurnally compared with other AD patients (all p < 0.05).ConclusionThe gut microbiota in AD patients exhibits disruptions of diurnal oscillation, which may provide novel insights into mechanisms of AD and the development of therapeutic strategies.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1127011/fullalcohol dependencediurnal oscillationgut microbiotadysbiosisrhythmicity |
| spellingShingle | Kangqing Zhao Zhaojun Ni Ying Qin Ran Zhu Zhoulong Yu Yundong Ma Wenhao Chen Qiqing Sun Zhong Wang Yanjing Liu Jingwen Zhao Wenjuan Peng Sifan Hu Jie Shi Jie Shi Jie Shi Lin Lu Hongqiang Sun Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence Frontiers in Cellular and Infection Microbiology alcohol dependence diurnal oscillation gut microbiota dysbiosis rhythmicity |
| title | Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence |
| title_full | Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence |
| title_fullStr | Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence |
| title_full_unstemmed | Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence |
| title_short | Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence |
| title_sort | disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence |
| topic | alcohol dependence diurnal oscillation gut microbiota dysbiosis rhythmicity |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1127011/full |
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