Exposure of Domestic Cats to Distinct <em>Ehrlichia canis</em> TRP Genotypes
Cats naturally exposed to <i>Ehrlichia canis</i> have been described in different regions of the world, but little is known about the genotypes associated with infection in these animals. To detect <i>E. canis</i>-specific antibodies and investigate the <i>E. canis</...
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MDPI AG
2021-12-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/8/12/310 |
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| author | Ísis Assis Braga Isis Indaiara Gonçalves Granjeiro Taques Estefânia Crivelatti Grontoski Ingrid Savino de Oliveira Dias Nathalia Assis Pereira Dirceu Guilherme de Souza Ramos Filipe Dantas-Torres Daniel Moura de Aguiar |
| author_facet | Ísis Assis Braga Isis Indaiara Gonçalves Granjeiro Taques Estefânia Crivelatti Grontoski Ingrid Savino de Oliveira Dias Nathalia Assis Pereira Dirceu Guilherme de Souza Ramos Filipe Dantas-Torres Daniel Moura de Aguiar |
| author_sort | Ísis Assis Braga |
| collection | DOAJ |
| description | Cats naturally exposed to <i>Ehrlichia canis</i> have been described in different regions of the world, but little is known about the genotypes associated with infection in these animals. To detect <i>E. canis</i>-specific antibodies and investigate the <i>E. canis</i> TRP genotypes in cats, serum samples from 76 domestic cats reactive to crude <i>E. canis</i> antigens by the indirect fluorescence antibody test (IFAT) were analyzed by ELISA, using <i>E. canis</i>-specific peptides (i.e., TRP19 and TRP36 /BR/US/CR). Of these, 25 (32.9%) cats reacted to at least one TRP peptide, confirming their specific exposure to <i>E. canis</i>. Eighteen (23.7%) cats reacted to TRP19, 15 (19.8%) to BRTRP36, and 11 (14.5%) to USTRP36, but none of them reacted to CRTRP36. Eight (10.5%) cats reacted to TRP19 but not to any TRP36 genotype, demonstrating the possible existence of a new <i>E. canis</i> genotype infecting felines. Nevertheless, this study provides the first report of anti-<i>E. canis</i>-specific antibodies in domestic cats. |
| first_indexed | 2024-03-10T03:56:39Z |
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| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T03:56:39Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-fb9221932d8b43b69f329b50076986972023-11-23T10:55:54ZengMDPI AGVeterinary Sciences2306-73812021-12-0181231010.3390/vetsci8120310Exposure of Domestic Cats to Distinct <em>Ehrlichia canis</em> TRP GenotypesÍsis Assis Braga0Isis Indaiara Gonçalves Granjeiro Taques1Estefânia Crivelatti Grontoski2Ingrid Savino de Oliveira Dias3Nathalia Assis Pereira4Dirceu Guilherme de Souza Ramos5Filipe Dantas-Torres6Daniel Moura de Aguiar7Veterinary Medicine College, Basic Unit of Bioscience, Mineiros University Center, 22nd Street s/n, Mineiros 75833-130, Goiás, BrazilVirology and Rickettsioses Laboratory, Department of Clinical Veterinary Medicine, Veterinary Medicine College, Federal University of Mato Grosso, Fernando Correa da Costa Avenue 2367, Cuiabá 78060-900, Mato Grosso, BrazilVirology and Rickettsioses Laboratory, Department of Clinical Veterinary Medicine, Veterinary Medicine College, Federal University of Mato Grosso, Fernando Correa da Costa Avenue 2367, Cuiabá 78060-900, Mato Grosso, BrazilVirology and Rickettsioses Laboratory, Department of Clinical Veterinary Medicine, Veterinary Medicine College, Federal University of Mato Grosso, Fernando Correa da Costa Avenue 2367, Cuiabá 78060-900, Mato Grosso, BrazilVirology and Rickettsioses Laboratory, Department of Clinical Veterinary Medicine, Veterinary Medicine College, Federal University of Mato Grosso, Fernando Correa da Costa Avenue 2367, Cuiabá 78060-900, Mato Grosso, BrazilLaboratory of Veterinary Pathology and Parasitology, Academic Unit of Agricultural Sciences, Federal University of Jataí, Jataí 75801-615, Goiás, BrazilDepartment of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Professor Moraes Rego Avenue s/n, Recife 50670-420, Pernambuco, BrazilVirology and Rickettsioses Laboratory, Department of Clinical Veterinary Medicine, Veterinary Medicine College, Federal University of Mato Grosso, Fernando Correa da Costa Avenue 2367, Cuiabá 78060-900, Mato Grosso, BrazilCats naturally exposed to <i>Ehrlichia canis</i> have been described in different regions of the world, but little is known about the genotypes associated with infection in these animals. To detect <i>E. canis</i>-specific antibodies and investigate the <i>E. canis</i> TRP genotypes in cats, serum samples from 76 domestic cats reactive to crude <i>E. canis</i> antigens by the indirect fluorescence antibody test (IFAT) were analyzed by ELISA, using <i>E. canis</i>-specific peptides (i.e., TRP19 and TRP36 /BR/US/CR). Of these, 25 (32.9%) cats reacted to at least one TRP peptide, confirming their specific exposure to <i>E. canis</i>. Eighteen (23.7%) cats reacted to TRP19, 15 (19.8%) to BRTRP36, and 11 (14.5%) to USTRP36, but none of them reacted to CRTRP36. Eight (10.5%) cats reacted to TRP19 but not to any TRP36 genotype, demonstrating the possible existence of a new <i>E. canis</i> genotype infecting felines. Nevertheless, this study provides the first report of anti-<i>E. canis</i>-specific antibodies in domestic cats.https://www.mdpi.com/2306-7381/8/12/310ELISAehrlichiosisfelineIFATserodiagnosis |
| spellingShingle | Ísis Assis Braga Isis Indaiara Gonçalves Granjeiro Taques Estefânia Crivelatti Grontoski Ingrid Savino de Oliveira Dias Nathalia Assis Pereira Dirceu Guilherme de Souza Ramos Filipe Dantas-Torres Daniel Moura de Aguiar Exposure of Domestic Cats to Distinct <em>Ehrlichia canis</em> TRP Genotypes Veterinary Sciences ELISA ehrlichiosis feline IFAT serodiagnosis |
| title | Exposure of Domestic Cats to Distinct <em>Ehrlichia canis</em> TRP Genotypes |
| title_full | Exposure of Domestic Cats to Distinct <em>Ehrlichia canis</em> TRP Genotypes |
| title_fullStr | Exposure of Domestic Cats to Distinct <em>Ehrlichia canis</em> TRP Genotypes |
| title_full_unstemmed | Exposure of Domestic Cats to Distinct <em>Ehrlichia canis</em> TRP Genotypes |
| title_short | Exposure of Domestic Cats to Distinct <em>Ehrlichia canis</em> TRP Genotypes |
| title_sort | exposure of domestic cats to distinct em ehrlichia canis em trp genotypes |
| topic | ELISA ehrlichiosis feline IFAT serodiagnosis |
| url | https://www.mdpi.com/2306-7381/8/12/310 |
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