Zoobooth: A portable, open-source and affordable approach for repeated size measurements of live individual zooplankton
Repeated size measurements of individual animals are valuable data for many research questions, but it is often hard to obtain without causing stress or damage to the animal. We developed a video-based approach called Zoobooth to size individual zooplankton, which involves a low risk of handling acc...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023025902 |
| _version_ | 1797815750425051136 |
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| author | Catharina Broch Jan Heuschele |
| author_facet | Catharina Broch Jan Heuschele |
| author_sort | Catharina Broch |
| collection | DOAJ |
| description | Repeated size measurements of individual animals are valuable data for many research questions, but it is often hard to obtain without causing stress or damage to the animal. We developed a video-based approach called Zoobooth to size individual zooplankton, which involves a low risk of handling accidents and stress. Here we describe the process of assembling the instrument we used to acquire video recordings of single zooplankton and the procedure to obtain size estimates from the recorded videos. Our setup produces accurate size estimates for Daphnia magna (correlation to manual measurements = 0.97), and was also tested with other zooplankton species. Zoobooth is especially advantageous when one needs size measurements of live, individual mesozooplankton. The device is small, portable, and comprised of very affordable and readily available components. It can easily be modified for other purposes, such as studies of coloration or behavior of micro-and macro-plankton. We share all the files to build and use Zoobooth. |
| first_indexed | 2024-03-13T08:27:25Z |
| format | Article |
| id | doaj.art-fb93b0bc45324beb8f085d52677da930 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:27:25Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-fb93b0bc45324beb8f085d52677da9302023-05-31T04:44:33ZengElsevierHeliyon2405-84402023-05-0195e15383Zoobooth: A portable, open-source and affordable approach for repeated size measurements of live individual zooplanktonCatharina Broch0Jan Heuschele1Centre for Ecological and Evolutionary Synthesis, Department of Biosciences, University of Oslo, Oslo, Norway; Section for Aquatic Biology and Toxicology, Department of Biosciences, University of Oslo, Oslo, NorwaySection for Aquatic Biology and Toxicology, Department of Biosciences, University of Oslo, Oslo, Norway; Centre for Biogeochemistry in the Anthropocene, The Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway; Corresponding author. Section for Aquatic Biology and Toxicology, Department of Biosciences, University of Oslo, Blindernveien 31, Blindern, 0371, Oslo, Norway.Repeated size measurements of individual animals are valuable data for many research questions, but it is often hard to obtain without causing stress or damage to the animal. We developed a video-based approach called Zoobooth to size individual zooplankton, which involves a low risk of handling accidents and stress. Here we describe the process of assembling the instrument we used to acquire video recordings of single zooplankton and the procedure to obtain size estimates from the recorded videos. Our setup produces accurate size estimates for Daphnia magna (correlation to manual measurements = 0.97), and was also tested with other zooplankton species. Zoobooth is especially advantageous when one needs size measurements of live, individual mesozooplankton. The device is small, portable, and comprised of very affordable and readily available components. It can easily be modified for other purposes, such as studies of coloration or behavior of micro-and macro-plankton. We share all the files to build and use Zoobooth.http://www.sciencedirect.com/science/article/pii/S2405844023025902Body sizeRepeated measurementsHandling riskStressVideo analysisZooplankton |
| spellingShingle | Catharina Broch Jan Heuschele Zoobooth: A portable, open-source and affordable approach for repeated size measurements of live individual zooplankton Heliyon Body size Repeated measurements Handling risk Stress Video analysis Zooplankton |
| title | Zoobooth: A portable, open-source and affordable approach for repeated size measurements of live individual zooplankton |
| title_full | Zoobooth: A portable, open-source and affordable approach for repeated size measurements of live individual zooplankton |
| title_fullStr | Zoobooth: A portable, open-source and affordable approach for repeated size measurements of live individual zooplankton |
| title_full_unstemmed | Zoobooth: A portable, open-source and affordable approach for repeated size measurements of live individual zooplankton |
| title_short | Zoobooth: A portable, open-source and affordable approach for repeated size measurements of live individual zooplankton |
| title_sort | zoobooth a portable open source and affordable approach for repeated size measurements of live individual zooplankton |
| topic | Body size Repeated measurements Handling risk Stress Video analysis Zooplankton |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023025902 |
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