Effects of TGF-β1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress
Introduction. This study aimed to explore the effects of TGF-β1 on regulating activities of cementoblasts and osteoblasts with or without stress. Material and Methods. Human recombinant TGF-β1 was added with different doses. Immunohistochemical test of osteoprotegerin (OPG)/receptor activator of nuc...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/718180 |
| _version_ | 1798043867222638592 |
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| author | Xianrui Yang Yanmin Wang Xianglong Han Rui Shu Tian Chen Huan Zeng Xin Xu Lan Huang Aishu Ren Jinlin Song Li Cao Ding Bai |
| author_facet | Xianrui Yang Yanmin Wang Xianglong Han Rui Shu Tian Chen Huan Zeng Xin Xu Lan Huang Aishu Ren Jinlin Song Li Cao Ding Bai |
| author_sort | Xianrui Yang |
| collection | DOAJ |
| description | Introduction. This study aimed to explore the effects of TGF-β1 on regulating activities of cementoblasts and osteoblasts with or without stress. Material and Methods. Human recombinant TGF-β1 was added with different doses. Immunohistochemical test of osteoprotegerin (OPG)/receptor activator of nuclear factor-kappaB ligand (RANKL) and Alizarin Red-S staining were conducted. Mechanical compressive stress was obtained by increasing the pressure of gaseous phase. OPG/RANKL expression was detected in both cells through quantitative real-time PCR. Results. Similar significant differences (P<0.05) existed in OPG/RANKL change with increasing concentration of TGF-β1 without mechanical stress for cementoblasts and osteoblasts. However, under 3 h stress, OPG increased and RANKL decreased significantly (P<0.01) but with similar OPG/RANKL change. Moreover, under 24 h stress, OPG change exhibited no difference (P>0.05), but RANKL decreased significantly (P<0.01) at 10 and 100 ng/mL TGF-β1 in cementoblasts. In osteoblasts, OPG increased significantly (P<0.01) at 10 and 100 ng/mL, whereas RANKL decreased with statistical difference (P<0.05) at 1 and 10 ng/mL. Conclusions. The effects of TGF-β1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar even without mechanical stress. However, these effects are different under mechanical compressive stress. |
| first_indexed | 2024-04-11T22:55:04Z |
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| id | doaj.art-fb9565ff952241e8b921c468674b242a |
| institution | Directory Open Access Journal |
| issn | 2356-6140 1537-744X |
| language | English |
| last_indexed | 2024-04-11T22:55:04Z |
| publishDate | 2015-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj.art-fb9565ff952241e8b921c468674b242a2022-12-22T03:58:28ZengHindawi LimitedThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/718180718180Effects of TGF-β1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive StressXianrui Yang0Yanmin Wang1Xianglong Han2Rui Shu3Tian Chen4Huan Zeng5Xin Xu6Lan Huang7Aishu Ren8Jinlin Song9Li Cao10Ding Bai11State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaChongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, ChinaChongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, ChinaChongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, ChinaChongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, ChinaState Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaIntroduction. This study aimed to explore the effects of TGF-β1 on regulating activities of cementoblasts and osteoblasts with or without stress. Material and Methods. Human recombinant TGF-β1 was added with different doses. Immunohistochemical test of osteoprotegerin (OPG)/receptor activator of nuclear factor-kappaB ligand (RANKL) and Alizarin Red-S staining were conducted. Mechanical compressive stress was obtained by increasing the pressure of gaseous phase. OPG/RANKL expression was detected in both cells through quantitative real-time PCR. Results. Similar significant differences (P<0.05) existed in OPG/RANKL change with increasing concentration of TGF-β1 without mechanical stress for cementoblasts and osteoblasts. However, under 3 h stress, OPG increased and RANKL decreased significantly (P<0.01) but with similar OPG/RANKL change. Moreover, under 24 h stress, OPG change exhibited no difference (P>0.05), but RANKL decreased significantly (P<0.01) at 10 and 100 ng/mL TGF-β1 in cementoblasts. In osteoblasts, OPG increased significantly (P<0.01) at 10 and 100 ng/mL, whereas RANKL decreased with statistical difference (P<0.05) at 1 and 10 ng/mL. Conclusions. The effects of TGF-β1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar even without mechanical stress. However, these effects are different under mechanical compressive stress.http://dx.doi.org/10.1155/2015/718180 |
| spellingShingle | Xianrui Yang Yanmin Wang Xianglong Han Rui Shu Tian Chen Huan Zeng Xin Xu Lan Huang Aishu Ren Jinlin Song Li Cao Ding Bai Effects of TGF-β1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress The Scientific World Journal |
| title | Effects of TGF-β1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress |
| title_full | Effects of TGF-β1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress |
| title_fullStr | Effects of TGF-β1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress |
| title_full_unstemmed | Effects of TGF-β1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress |
| title_short | Effects of TGF-β1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress |
| title_sort | effects of tgf β1 on opg rankl expression of cementoblasts and osteoblasts are similar without stress but different with mechanical compressive stress |
| url | http://dx.doi.org/10.1155/2015/718180 |
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