Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)
Plant U-box (PUB) proteins belong to a class of ubiquitin ligases essential in various biological processes. Sesame (Sesamum indicum L.) is an important and worldwide cultivated oilseed crop. However few studies have been conducted to explore the role of PUBs in drought tolerance in sesame. This stu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1261238/full |
| _version_ | 1827318432198033408 |
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| author | Hengchun Cao Hengchun Cao Hengchun Cao Qiuzhen Tian Qiuzhen Tian Qiuzhen Tian Ming Ju Ming Ju Ming Ju Yinghui Duan Yinghui Duan Yinghui Duan Guiting Li Guiting Li Guiting Li Qin Ma Qin Ma Qin Ma Haiyang Zhang Haiyang Zhang Haiyang Zhang Xianmei Zhang Xianmei Zhang Hongmei Miao Hongmei Miao Hongmei Miao |
| author_facet | Hengchun Cao Hengchun Cao Hengchun Cao Qiuzhen Tian Qiuzhen Tian Qiuzhen Tian Ming Ju Ming Ju Ming Ju Yinghui Duan Yinghui Duan Yinghui Duan Guiting Li Guiting Li Guiting Li Qin Ma Qin Ma Qin Ma Haiyang Zhang Haiyang Zhang Haiyang Zhang Xianmei Zhang Xianmei Zhang Hongmei Miao Hongmei Miao Hongmei Miao |
| author_sort | Hengchun Cao |
| collection | DOAJ |
| description | Plant U-box (PUB) proteins belong to a class of ubiquitin ligases essential in various biological processes. Sesame (Sesamum indicum L.) is an important and worldwide cultivated oilseed crop. However few studies have been conducted to explore the role of PUBs in drought tolerance in sesame. This study identified a total of 56 members of the sesame PUB family (SiPUB) genes distributed unevenly across all 13 chromosomes. Based on phylogenetic analysis, all 56 SiPUB genes were classified into six groups with various structures and motifs. Cis-acting element analysis suggested that the SiPUB genes are involved in response to various stresses including drought. Based on RNA-seq analysis and quantitative real-time PCR, we identified nine SiPUB genes with significantly different expression profiles under drought stress. The expression patterns of six SiPUB genes in root, leaf and stem tissues corroborated the reliability of the RNA-seq datasets. These findings underscore the importance of SiPUB genes in enhancing drought tolerance in sesame plants. Our study provides novel insights into the evolutionary patterns and variations of PUB genes in sesame and lays the foundation for comprehending the functional characteristics of SiPUB genes under drought-induced stress conditions. |
| first_indexed | 2024-03-11T23:35:33Z |
| format | Article |
| id | doaj.art-fb97c6f8f35746e89aa733bbff5e97fe |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-24T23:58:53Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-fb97c6f8f35746e89aa733bbff5e97fe2024-03-14T10:51:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-09-011410.3389/fpls.2023.12612381261238Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.)Hengchun Cao0Hengchun Cao1Hengchun Cao2Qiuzhen Tian3Qiuzhen Tian4Qiuzhen Tian5Ming Ju6Ming Ju7Ming Ju8Yinghui Duan9Yinghui Duan10Yinghui Duan11Guiting Li12Guiting Li13Guiting Li14Qin Ma15Qin Ma16Qin Ma17Haiyang Zhang18Haiyang Zhang19Haiyang Zhang20Xianmei Zhang21Xianmei Zhang22Hongmei Miao23Hongmei Miao24Hongmei Miao25Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaHenan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaHenan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaHenan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaHenan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaHenan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaHenan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaLuohe Academy of Agricultural Sciences, Luohe, Henan, ChinaHenan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaKey Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaPlant U-box (PUB) proteins belong to a class of ubiquitin ligases essential in various biological processes. Sesame (Sesamum indicum L.) is an important and worldwide cultivated oilseed crop. However few studies have been conducted to explore the role of PUBs in drought tolerance in sesame. This study identified a total of 56 members of the sesame PUB family (SiPUB) genes distributed unevenly across all 13 chromosomes. Based on phylogenetic analysis, all 56 SiPUB genes were classified into six groups with various structures and motifs. Cis-acting element analysis suggested that the SiPUB genes are involved in response to various stresses including drought. Based on RNA-seq analysis and quantitative real-time PCR, we identified nine SiPUB genes with significantly different expression profiles under drought stress. The expression patterns of six SiPUB genes in root, leaf and stem tissues corroborated the reliability of the RNA-seq datasets. These findings underscore the importance of SiPUB genes in enhancing drought tolerance in sesame plants. Our study provides novel insights into the evolutionary patterns and variations of PUB genes in sesame and lays the foundation for comprehending the functional characteristics of SiPUB genes under drought-induced stress conditions.https://www.frontiersin.org/articles/10.3389/fpls.2023.1261238/fullubiquitinSesamum indicumPUBdrought tolerancegene family |
| spellingShingle | Hengchun Cao Hengchun Cao Hengchun Cao Qiuzhen Tian Qiuzhen Tian Qiuzhen Tian Ming Ju Ming Ju Ming Ju Yinghui Duan Yinghui Duan Yinghui Duan Guiting Li Guiting Li Guiting Li Qin Ma Qin Ma Qin Ma Haiyang Zhang Haiyang Zhang Haiyang Zhang Xianmei Zhang Xianmei Zhang Hongmei Miao Hongmei Miao Hongmei Miao Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.) Frontiers in Plant Science ubiquitin Sesamum indicum PUB drought tolerance gene family |
| title | Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.) |
| title_full | Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.) |
| title_fullStr | Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.) |
| title_full_unstemmed | Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.) |
| title_short | Genome-wide analysis of the U-box E3 ubiquitin ligase family role in drought tolerance in sesame (Sesamum indicum L.) |
| title_sort | genome wide analysis of the u box e3 ubiquitin ligase family role in drought tolerance in sesame sesamum indicum l |
| topic | ubiquitin Sesamum indicum PUB drought tolerance gene family |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1261238/full |
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