Characterization of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Breeders with Colibacillosis in Mississippi
Avian pathogenic <i>Escherichia coli</i> (APEC) causes colibacillosis in poultry, a leading cause of poultry mortality worldwide. It is crucial to control APEC in broiler breeders as it is vertically transferred to progeny via eggs. However, there is only limited knowledge on the current...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Poultry |
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| Online Access: | https://www.mdpi.com/2674-1164/2/1/4 |
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| author | Jiddu Joseph Madalyn Jennings Nicolle Barbieri Li Zhang Pratima Adhikari Reshma Ramachandran |
| author_facet | Jiddu Joseph Madalyn Jennings Nicolle Barbieri Li Zhang Pratima Adhikari Reshma Ramachandran |
| author_sort | Jiddu Joseph |
| collection | DOAJ |
| description | Avian pathogenic <i>Escherichia coli</i> (APEC) causes colibacillosis in poultry, a leading cause of poultry mortality worldwide. It is crucial to control APEC in broiler breeders as it is vertically transferred to progeny via eggs. However, there is only limited knowledge on the current APEC population in breeders. This study characterized 28 APEC strains isolated from broiler breeders with colibacillosis. The genotypic-virulence characteristics as well as antimicrobial and heavy-metal resistance patterns of the isolates were determined. Results showed that O88 is the most prevalent serogroup and B2 is the predominant phylogenetic group. Among virulence genes, genes for iron acquisition (<i>iroN</i> and <i>iutA</i>), protectins (<i>iss</i> and <i>ompT</i>), and toxin production (<i>hlyF</i>) exhibited the highest prevalence. Further, 93% of the isolates carried at least one antimicrobial resistance gene with highest prevalence for tetracycline gene <i>tetA</i>. Among the isolates, 10.71% exhibited multidrug resistance. All isolates carried at least one heavy-metal resistance gene with the highest prevalence for arsenic gene <i>arsC</i> and the highest resistance towards silver. Our findings provide insight into the characteristics of current APEC populations in broiler breeders in Mississippi. This will help future research on the pathogenesis of APEC and the development of effective prevention and control strategies against APEC in broiler breeders. |
| first_indexed | 2024-03-11T05:59:46Z |
| format | Article |
| id | doaj.art-fb97f249afc74546a03d166be76f75a8 |
| institution | Directory Open Access Journal |
| issn | 2674-1164 |
| language | English |
| last_indexed | 2024-03-11T05:59:46Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Poultry |
| spelling | doaj.art-fb97f249afc74546a03d166be76f75a82023-11-17T13:27:48ZengMDPI AGPoultry2674-11642023-01-0121243910.3390/poultry2010004Characterization of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Breeders with Colibacillosis in MississippiJiddu Joseph0Madalyn Jennings1Nicolle Barbieri2Li Zhang3Pratima Adhikari4Reshma Ramachandran5Department of Poultry Science, Mississippi State University, Starkville, MS 39762, USADepartment of Poultry Science, Mississippi State University, Starkville, MS 39762, USABoehringer Ingelheim, Ridgefield, CT 06877, USADepartment of Poultry Science, Mississippi State University, Starkville, MS 39762, USADepartment of Poultry Science, Mississippi State University, Starkville, MS 39762, USADepartment of Poultry Science, Mississippi State University, Starkville, MS 39762, USAAvian pathogenic <i>Escherichia coli</i> (APEC) causes colibacillosis in poultry, a leading cause of poultry mortality worldwide. It is crucial to control APEC in broiler breeders as it is vertically transferred to progeny via eggs. However, there is only limited knowledge on the current APEC population in breeders. This study characterized 28 APEC strains isolated from broiler breeders with colibacillosis. The genotypic-virulence characteristics as well as antimicrobial and heavy-metal resistance patterns of the isolates were determined. Results showed that O88 is the most prevalent serogroup and B2 is the predominant phylogenetic group. Among virulence genes, genes for iron acquisition (<i>iroN</i> and <i>iutA</i>), protectins (<i>iss</i> and <i>ompT</i>), and toxin production (<i>hlyF</i>) exhibited the highest prevalence. Further, 93% of the isolates carried at least one antimicrobial resistance gene with highest prevalence for tetracycline gene <i>tetA</i>. Among the isolates, 10.71% exhibited multidrug resistance. All isolates carried at least one heavy-metal resistance gene with the highest prevalence for arsenic gene <i>arsC</i> and the highest resistance towards silver. Our findings provide insight into the characteristics of current APEC populations in broiler breeders in Mississippi. This will help future research on the pathogenesis of APEC and the development of effective prevention and control strategies against APEC in broiler breeders.https://www.mdpi.com/2674-1164/2/1/4APECbroiler breedergenotypic characterizationantibiotic resistancemetal resistance |
| spellingShingle | Jiddu Joseph Madalyn Jennings Nicolle Barbieri Li Zhang Pratima Adhikari Reshma Ramachandran Characterization of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Breeders with Colibacillosis in Mississippi Poultry APEC broiler breeder genotypic characterization antibiotic resistance metal resistance |
| title | Characterization of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Breeders with Colibacillosis in Mississippi |
| title_full | Characterization of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Breeders with Colibacillosis in Mississippi |
| title_fullStr | Characterization of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Breeders with Colibacillosis in Mississippi |
| title_full_unstemmed | Characterization of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Breeders with Colibacillosis in Mississippi |
| title_short | Characterization of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Breeders with Colibacillosis in Mississippi |
| title_sort | characterization of avian pathogenic i escherichia coli i isolated from broiler breeders with colibacillosis in mississippi |
| topic | APEC broiler breeder genotypic characterization antibiotic resistance metal resistance |
| url | https://www.mdpi.com/2674-1164/2/1/4 |
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