Emerging Viral Pathogens in Sturgeon Aquaculture in Poland: Focus on Herpesviruses and Mimivirus Detection
Recently, Poland has become a leading producer of sturgeon meat and caviar in Europe and is one of the largest in the world. The growing importance of this branch of aquaculture means that diseases of these fish, especially viral ones, are becoming the object of interest for ichthyopathologists. In...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/8/1496 |
| _version_ | 1797521903353593856 |
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| author | Magdalena Stachnik Marek Matras Ewa Borzym Joanna Maj-Paluch Michał Reichert |
| author_facet | Magdalena Stachnik Marek Matras Ewa Borzym Joanna Maj-Paluch Michał Reichert |
| author_sort | Magdalena Stachnik |
| collection | DOAJ |
| description | Recently, Poland has become a leading producer of sturgeon meat and caviar in Europe and is one of the largest in the world. The growing importance of this branch of aquaculture means that diseases of these fish, especially viral ones, are becoming the object of interest for ichthyopathologists. In recent years, there have been increasing reports of health problems in the dynamically developing sturgeon farming. The greatest risk appears to be emerging infectious diseases that are caused by viruses and that can become a serious threat to the development of the aquaculture industry and the success of sturgeon restitution programs undertaken in many European countries, including Poland. In this paper, an attempt was made to determine the spread of the two most important groups of viruses in Polish sturgeon farming: These include the herpesviruses and sturgeon nucleocytoplasmic large DNA viruses (sNCLDV), in particular, mimiviruses. In the years 2016–2020, 136 samples from nine farms were collected and tested by using the WSSK-1 cell line, PCR and Real Time PCR methods. All results were negative for herpesviruses. Out of the samples, 26% of the samples have been tested positive for mimiviruses. Sanger sequencing of mimiviruses demonstrated their affiliation with AciV-E. The sequence characterization confirmed the presence of both V1 and V2 lineages in Polish fish facilities, but variant V2 seems to be more widespread, as is observed in other European countries. |
| first_indexed | 2024-03-10T08:19:07Z |
| format | Article |
| id | doaj.art-fb9d7a18b1f14c959adf151408d8d36b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T08:19:07Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-fb9d7a18b1f14c959adf151408d8d36b2023-11-22T10:10:15ZengMDPI AGViruses1999-49152021-07-01138149610.3390/v13081496Emerging Viral Pathogens in Sturgeon Aquaculture in Poland: Focus on Herpesviruses and Mimivirus DetectionMagdalena Stachnik0Marek Matras1Ewa Borzym2Joanna Maj-Paluch3Michał Reichert4Department of Fish Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment of Fish Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment of Fish Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment of Fish Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment of Fish Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandRecently, Poland has become a leading producer of sturgeon meat and caviar in Europe and is one of the largest in the world. The growing importance of this branch of aquaculture means that diseases of these fish, especially viral ones, are becoming the object of interest for ichthyopathologists. In recent years, there have been increasing reports of health problems in the dynamically developing sturgeon farming. The greatest risk appears to be emerging infectious diseases that are caused by viruses and that can become a serious threat to the development of the aquaculture industry and the success of sturgeon restitution programs undertaken in many European countries, including Poland. In this paper, an attempt was made to determine the spread of the two most important groups of viruses in Polish sturgeon farming: These include the herpesviruses and sturgeon nucleocytoplasmic large DNA viruses (sNCLDV), in particular, mimiviruses. In the years 2016–2020, 136 samples from nine farms were collected and tested by using the WSSK-1 cell line, PCR and Real Time PCR methods. All results were negative for herpesviruses. Out of the samples, 26% of the samples have been tested positive for mimiviruses. Sanger sequencing of mimiviruses demonstrated their affiliation with AciV-E. The sequence characterization confirmed the presence of both V1 and V2 lineages in Polish fish facilities, but variant V2 seems to be more widespread, as is observed in other European countries.https://www.mdpi.com/1999-4915/13/8/1496sturgeonherpesvirusAciHV-1AciHV-2sNCLDVmimivirus |
| spellingShingle | Magdalena Stachnik Marek Matras Ewa Borzym Joanna Maj-Paluch Michał Reichert Emerging Viral Pathogens in Sturgeon Aquaculture in Poland: Focus on Herpesviruses and Mimivirus Detection Viruses sturgeon herpesvirus AciHV-1 AciHV-2 sNCLDV mimivirus |
| title | Emerging Viral Pathogens in Sturgeon Aquaculture in Poland: Focus on Herpesviruses and Mimivirus Detection |
| title_full | Emerging Viral Pathogens in Sturgeon Aquaculture in Poland: Focus on Herpesviruses and Mimivirus Detection |
| title_fullStr | Emerging Viral Pathogens in Sturgeon Aquaculture in Poland: Focus on Herpesviruses and Mimivirus Detection |
| title_full_unstemmed | Emerging Viral Pathogens in Sturgeon Aquaculture in Poland: Focus on Herpesviruses and Mimivirus Detection |
| title_short | Emerging Viral Pathogens in Sturgeon Aquaculture in Poland: Focus on Herpesviruses and Mimivirus Detection |
| title_sort | emerging viral pathogens in sturgeon aquaculture in poland focus on herpesviruses and mimivirus detection |
| topic | sturgeon herpesvirus AciHV-1 AciHV-2 sNCLDV mimivirus |
| url | https://www.mdpi.com/1999-4915/13/8/1496 |
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