Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
Abstract Background This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study. Methods On one side, rat tibiae were the...
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | Head & Face Medicine |
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| Online Access: | https://doi.org/10.1186/s13005-023-00349-2 |
| _version_ | 1797863438532214784 |
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| author | Kristian Kniha Eva Miriam Buhl Faruk Al-Sibai Stephan Christian Möhlhenrich Anna Bock Marius Heitzer Frank Hölzle Ali Modabber |
| author_facet | Kristian Kniha Eva Miriam Buhl Faruk Al-Sibai Stephan Christian Möhlhenrich Anna Bock Marius Heitzer Frank Hölzle Ali Modabber |
| author_sort | Kristian Kniha |
| collection | DOAJ |
| description | Abstract Background This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study. Methods On one side, rat tibiae were thermally treated before implant insertion. The contralateral side was used as the control group without tempering. Temperatures of 4 °C, 3 °C, 2 °C, 48 °C, 49 °C, and 50 °C were evaluated with a tempering time of 1 min. Energy-dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) analyses were performed. Results The EDX analysis revealed significant increases in element weights at 50 °C (e.g., calcium, phosphate, sodium, and sulfur; p < 0.01). The results of the TEM analysis showed that at all the applied cold and warm temperatures, signs of cell damage were observed, including vacuolization, shrinkage, and detachment from the surrounding bone matrix. Some cells became necrotic, leaving the lacunae empty. Conclusions Temperature of 50 °C led to irreversible cell death. The degree of damage was more significant at 50 °C and 2 °C than at 48 °C and 5 °C. Although this was a preliminary study, from the results, we identified that a temperature of 50 °C at a time interval of 60 min can lower the number of samples in a further study of thermo-explantation. Thus, the subsequent planned in vivo study in pigs, which will consider osseointegrated implants, is feasible. |
| first_indexed | 2024-04-09T22:36:38Z |
| format | Article |
| id | doaj.art-fb9f134eb1254feeb63c7597a257eac4 |
| institution | Directory Open Access Journal |
| issn | 1746-160X |
| language | English |
| last_indexed | 2024-04-09T22:36:38Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Head & Face Medicine |
| spelling | doaj.art-fb9f134eb1254feeb63c7597a257eac42023-03-22T12:30:02ZengBMCHead & Face Medicine1746-160X2023-03-011911710.1186/s13005-023-00349-2Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat studyKristian Kniha0Eva Miriam Buhl1Faruk Al-Sibai2Stephan Christian Möhlhenrich3Anna Bock4Marius Heitzer5Frank Hölzle6Ali Modabber7Department of Oral and Cranio-Maxillofacial Surgery, University Hospital RWTHInstitute for Pathology, Electron Microscopy Facility, University Hospital RWTHInstitute of Heat and Mass Transfer, University Hospital RWTHDepartment of Orthodontics, University of Witten/HerdeckeDepartment of Oral and Cranio-Maxillofacial Surgery, University Hospital RWTHDepartment of Oral and Cranio-Maxillofacial Surgery, University Hospital RWTHDepartment of Oral and Cranio-Maxillofacial Surgery, University Hospital RWTHDepartment of Oral and Cranio-Maxillofacial Surgery, University Hospital RWTHAbstract Background This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study. Methods On one side, rat tibiae were thermally treated before implant insertion. The contralateral side was used as the control group without tempering. Temperatures of 4 °C, 3 °C, 2 °C, 48 °C, 49 °C, and 50 °C were evaluated with a tempering time of 1 min. Energy-dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) analyses were performed. Results The EDX analysis revealed significant increases in element weights at 50 °C (e.g., calcium, phosphate, sodium, and sulfur; p < 0.01). The results of the TEM analysis showed that at all the applied cold and warm temperatures, signs of cell damage were observed, including vacuolization, shrinkage, and detachment from the surrounding bone matrix. Some cells became necrotic, leaving the lacunae empty. Conclusions Temperature of 50 °C led to irreversible cell death. The degree of damage was more significant at 50 °C and 2 °C than at 48 °C and 5 °C. Although this was a preliminary study, from the results, we identified that a temperature of 50 °C at a time interval of 60 min can lower the number of samples in a further study of thermo-explantation. Thus, the subsequent planned in vivo study in pigs, which will consider osseointegrated implants, is feasible.https://doi.org/10.1186/s13005-023-00349-2Osteocyte lacunaOsteonecrosisTemperatureHistopathology |
| spellingShingle | Kristian Kniha Eva Miriam Buhl Faruk Al-Sibai Stephan Christian Möhlhenrich Anna Bock Marius Heitzer Frank Hölzle Ali Modabber Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study Head & Face Medicine Osteocyte lacuna Osteonecrosis Temperature Histopathology |
| title | Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study |
| title_full | Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study |
| title_fullStr | Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study |
| title_full_unstemmed | Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study |
| title_short | Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study |
| title_sort | results of thermal osteonecrosis for implant removal on electron microscopy implant stability and radiographic parameters a rat study |
| topic | Osteocyte lacuna Osteonecrosis Temperature Histopathology |
| url | https://doi.org/10.1186/s13005-023-00349-2 |
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