Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer
MicroRNAs have emerged as new diagnostic and therapeutic biomarkers for breast cancer. Herein, we analysed miR-99a-5p expression levels in primary tumours and plasma of breast cancer patients to evaluate its usefulness as a minimally invasive diagnostic biomarker. MiR-99a-5p expression levels were d...
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MDPI AG
2020-10-01
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Online Access: | https://www.mdpi.com/1422-0067/21/19/7427 |
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author | Iris Garrido-Cano Vera Constâncio Anna Adam-Artigues Ana Lameirinhas Soraya Simón Belen Ortega María Teresa Martínez Cristina Hernando Begoña Bermejo Ana Lluch Paula Lopes Rui Henrique Carmen Jerónimo Juan Miguel Cejalvo Pilar Eroles |
author_facet | Iris Garrido-Cano Vera Constâncio Anna Adam-Artigues Ana Lameirinhas Soraya Simón Belen Ortega María Teresa Martínez Cristina Hernando Begoña Bermejo Ana Lluch Paula Lopes Rui Henrique Carmen Jerónimo Juan Miguel Cejalvo Pilar Eroles |
author_sort | Iris Garrido-Cano |
collection | DOAJ |
description | MicroRNAs have emerged as new diagnostic and therapeutic biomarkers for breast cancer. Herein, we analysed miR-99a-5p expression levels in primary tumours and plasma of breast cancer patients to evaluate its usefulness as a minimally invasive diagnostic biomarker. MiR-99a-5p expression levels were determined by quantitative real-time PCR in three independent cohorts of patients: (I) Discovery cohort: breast cancer tissues (<i>n</i> = 103) and healthy breast tissues (<i>n</i> = 26); (II) Testing cohort: plasma samples from 105 patients and 98 healthy donors; (III) Validation cohort: plasma samples from 89 patients and 85 healthy donors. Our results demonstrated that miR-99a-5p was significantly downregulated in breast cancer tissues compared to healthy breast tissues. Conversely, miR-99a-5p levels were significantly higher in breast cancer patients than in healthy controls in plasma samples from both testing and validation cohorts, and ROC curve analysis revealed that miR-99a-5p has good diagnostic potential even to detect early breast cancer. In conclusion, miR-99a-5p’s deregulated expression distinguished healthy patients from breast cancer patients in two different types of samples (tissues and plasma). Interestingly, expression levels in plasma were significantly lower in healthy controls than in early-stage breast cancer patients. Our findings suggest circulating miR-99a-5p as a novel promising non-invasive biomarker for breast cancer detection. |
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id | doaj.art-fb9f89c5132d44ec94f3c2b0577c86bd |
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issn | 1661-6596 1422-0067 |
language | English |
last_indexed | 2024-03-10T15:47:22Z |
publishDate | 2020-10-01 |
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series | International Journal of Molecular Sciences |
spelling | doaj.art-fb9f89c5132d44ec94f3c2b0577c86bd2023-11-20T16:24:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012119742710.3390/ijms21197427Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast CancerIris Garrido-Cano0Vera Constâncio1Anna Adam-Artigues2Ana Lameirinhas3Soraya Simón4Belen Ortega5María Teresa Martínez6Cristina Hernando7Begoña Bermejo8Ana Lluch9Paula Lopes10Rui Henrique11Carmen Jerónimo12Juan Miguel Cejalvo13Pilar Eroles14Biomedical Research Institute INCLIVA, 46010 Valencia, SpainCancer Biology and Epigenetics Group–Research Center, Portuguese Oncology Institute of Porto (CI-IPOP), 4200-072 Porto, PortugalBiomedical Research Institute INCLIVA, 46010 Valencia, SpainBiomedical Research Institute INCLIVA, 46010 Valencia, SpainBiomedical Research Institute INCLIVA, 46010 Valencia, SpainBiomedical Research Institute INCLIVA, 46010 Valencia, SpainBiomedical Research Institute INCLIVA, 46010 Valencia, SpainBiomedical Research Institute INCLIVA, 46010 Valencia, SpainBiomedical Research Institute INCLIVA, 46010 Valencia, SpainClinical Oncology Department, Hospital Clínico Universitario de Valencia, 46010 Valencia, SpainCancer Biology and Epigenetics Group–Research Center, Portuguese Oncology Institute of Porto (CI-IPOP), 4200-072 Porto, PortugalCancer Biology and Epigenetics Group–Research Center, Portuguese Oncology Institute of Porto (CI-IPOP), 4200-072 Porto, PortugalCancer Biology and Epigenetics Group–Research Center, Portuguese Oncology Institute of Porto (CI-IPOP), 4200-072 Porto, PortugalBiomedical Research Institute INCLIVA, 46010 Valencia, SpainBiomedical Research Institute INCLIVA, 46010 Valencia, SpainMicroRNAs have emerged as new diagnostic and therapeutic biomarkers for breast cancer. Herein, we analysed miR-99a-5p expression levels in primary tumours and plasma of breast cancer patients to evaluate its usefulness as a minimally invasive diagnostic biomarker. MiR-99a-5p expression levels were determined by quantitative real-time PCR in three independent cohorts of patients: (I) Discovery cohort: breast cancer tissues (<i>n</i> = 103) and healthy breast tissues (<i>n</i> = 26); (II) Testing cohort: plasma samples from 105 patients and 98 healthy donors; (III) Validation cohort: plasma samples from 89 patients and 85 healthy donors. Our results demonstrated that miR-99a-5p was significantly downregulated in breast cancer tissues compared to healthy breast tissues. Conversely, miR-99a-5p levels were significantly higher in breast cancer patients than in healthy controls in plasma samples from both testing and validation cohorts, and ROC curve analysis revealed that miR-99a-5p has good diagnostic potential even to detect early breast cancer. In conclusion, miR-99a-5p’s deregulated expression distinguished healthy patients from breast cancer patients in two different types of samples (tissues and plasma). Interestingly, expression levels in plasma were significantly lower in healthy controls than in early-stage breast cancer patients. Our findings suggest circulating miR-99a-5p as a novel promising non-invasive biomarker for breast cancer detection.https://www.mdpi.com/1422-0067/21/19/7427breast cancerbiomarkerplasmadiagnosis |
spellingShingle | Iris Garrido-Cano Vera Constâncio Anna Adam-Artigues Ana Lameirinhas Soraya Simón Belen Ortega María Teresa Martínez Cristina Hernando Begoña Bermejo Ana Lluch Paula Lopes Rui Henrique Carmen Jerónimo Juan Miguel Cejalvo Pilar Eroles Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer International Journal of Molecular Sciences breast cancer biomarker plasma diagnosis |
title | Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer |
title_full | Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer |
title_fullStr | Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer |
title_full_unstemmed | Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer |
title_short | Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer |
title_sort | circulating mir 99a 5p expression in plasma a potential biomarker for early diagnosis of breast cancer |
topic | breast cancer biomarker plasma diagnosis |
url | https://www.mdpi.com/1422-0067/21/19/7427 |
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