Short-term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats model
Abstract Background Vascular dementia is the second dementing illness after Alzheimer’s disease and caused by reduced blood flow to the brain, and affects cognitive abilities. Our previous study found that auricular electrical stimulation (ES) improved motor and learning impairment, and this phenome...
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| 格式: | 文件 |
| 语言: | English |
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BMC
2019-05-01
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| 丛编: | Journal of Biomedical Science |
| 主题: | |
| 在线阅读: | http://link.springer.com/article/10.1186/s12929-019-0526-9 |
| _version_ | 1828493319060062208 |
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| author | Tai-Hsiang Huang Yi-Wen Lin Chun-Ping Huang Jing-Ming Chen Ching-Liang Hsieh |
| author_facet | Tai-Hsiang Huang Yi-Wen Lin Chun-Ping Huang Jing-Ming Chen Ching-Liang Hsieh |
| author_sort | Tai-Hsiang Huang |
| collection | DOAJ |
| description | Abstract Background Vascular dementia is the second dementing illness after Alzheimer’s disease and caused by reduced blood flow to the brain, and affects cognitive abilities. Our previous study found that auricular electrical stimulation (ES) improved motor and learning impairment, and this phenomenon related with nicotinic acetylcholine receptor (nAChR) expressed cells. However, the underlying mechanism was not clear. In the present study, we investigated the effects of auricular ES on cortical blood flow (CBF) and acetylcholine (ACh) - nAChRs expressed cells. Methods Vascular dementia rat animal model was established by permanent occlusions of common carotid arteries with 6–0 nylon suture filament. At 21 day after surgery, motor impairment was confirmed by rotarod test. 15-Hz auricular ES were applied to the ears for 20 min and CBF was recorded at the mean time. The brains were immediately dissected for immunohistochemical stain and western blot analysis. Results Our results showed that 15-Hz auricular ES rapidly elevated CBF in the middle cerebral artery. The numbers of nAChR α4 immuno-positive cells and western blot levels were significally increased by 15-Hz auricular ES in the hippocampal CA2 output cortex. The numbers of choline acetyltransferase (ChAT) – a key enzyme for biosynthesis of ACh – immuno-positive cells and western blot levels had no significant differences. Conclusions The present data suggested that the 15-Hz auricular ES for 20 min rapidly elevated cortical blood flow, promoted the expression of nAChR α4, and would be beneficial for the treatment of Alzheimer type and vascular type dementia. |
| first_indexed | 2024-12-11T11:35:45Z |
| format | Article |
| id | doaj.art-fb9fbae64ee94523ba2543a0c1ee4a3c |
| institution | Directory Open Access Journal |
| issn | 1423-0127 |
| language | English |
| last_indexed | 2024-12-11T11:35:45Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-fb9fbae64ee94523ba2543a0c1ee4a3c2022-12-22T01:08:45ZengBMCJournal of Biomedical Science1423-01272019-05-012611810.1186/s12929-019-0526-9Short-term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats modelTai-Hsiang Huang0Yi-Wen Lin1Chun-Ping Huang2Jing-Ming Chen3Ching-Liang Hsieh4Graduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical UniversityGraduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical UniversityChinese Medicine Research Center, China Medical UniversityGraduate Institute of Applied Science and Engineering, Fu Jen Catholic UniversityChinese Medicine Research Center, China Medical UniversityAbstract Background Vascular dementia is the second dementing illness after Alzheimer’s disease and caused by reduced blood flow to the brain, and affects cognitive abilities. Our previous study found that auricular electrical stimulation (ES) improved motor and learning impairment, and this phenomenon related with nicotinic acetylcholine receptor (nAChR) expressed cells. However, the underlying mechanism was not clear. In the present study, we investigated the effects of auricular ES on cortical blood flow (CBF) and acetylcholine (ACh) - nAChRs expressed cells. Methods Vascular dementia rat animal model was established by permanent occlusions of common carotid arteries with 6–0 nylon suture filament. At 21 day after surgery, motor impairment was confirmed by rotarod test. 15-Hz auricular ES were applied to the ears for 20 min and CBF was recorded at the mean time. The brains were immediately dissected for immunohistochemical stain and western blot analysis. Results Our results showed that 15-Hz auricular ES rapidly elevated CBF in the middle cerebral artery. The numbers of nAChR α4 immuno-positive cells and western blot levels were significally increased by 15-Hz auricular ES in the hippocampal CA2 output cortex. The numbers of choline acetyltransferase (ChAT) – a key enzyme for biosynthesis of ACh – immuno-positive cells and western blot levels had no significant differences. Conclusions The present data suggested that the 15-Hz auricular ES for 20 min rapidly elevated cortical blood flow, promoted the expression of nAChR α4, and would be beneficial for the treatment of Alzheimer type and vascular type dementia.http://link.springer.com/article/10.1186/s12929-019-0526-9DementiaBilateral carotid artery occlusionAuricular electrical stimulationCerebral blood flowAcetylcholine receptor |
| spellingShingle | Tai-Hsiang Huang Yi-Wen Lin Chun-Ping Huang Jing-Ming Chen Ching-Liang Hsieh Short-term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats model Journal of Biomedical Science Dementia Bilateral carotid artery occlusion Auricular electrical stimulation Cerebral blood flow Acetylcholine receptor |
| title | Short-term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats model |
| title_full | Short-term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats model |
| title_fullStr | Short-term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats model |
| title_full_unstemmed | Short-term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats model |
| title_short | Short-term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats model |
| title_sort | short term auricular electrical stimulation rapidly elevated cortical blood flow and promoted the expression of nicotinic acetylcholine receptor α4 in the 2 vessel occlusion rats model |
| topic | Dementia Bilateral carotid artery occlusion Auricular electrical stimulation Cerebral blood flow Acetylcholine receptor |
| url | http://link.springer.com/article/10.1186/s12929-019-0526-9 |
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