Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus
<em><span lang="EN-US">Rigidoporus lignosus </span></em><span lang="EN-US">is the most important pathogen of rubber tree which causes white root rot disease. The use of antagonistic microbe is recommended to control this pathogen. This research was c...
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| Format: | Article |
| Language: | Indonesian |
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Perhimpunan Fitopatologi Indonesia
2018-01-01
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| Series: | Jurnal Fitopatologi Indonesia |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/jfiti/article/view/19632 |
| _version_ | 1818128208658169856 |
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| author | Siti Hardiyanti Bonny Poernomo Wahyu Soekarno Titiek Siti Yuliani |
| author_facet | Siti Hardiyanti Bonny Poernomo Wahyu Soekarno Titiek Siti Yuliani |
| author_sort | Siti Hardiyanti |
| collection | DOAJ |
| description | <em><span lang="EN-US">Rigidoporus lignosus </span></em><span lang="EN-US">is the most important pathogen of rubber tree which causes white root rot disease. The use of antagonistic microbe is recommended to control this pathogen. This research was conducted to isolate endophytic and rhizospheric microbes, and to study their ability to inhibit growth of <em>R. lignosus</em>. Research consisted of isolation of endophytic and rhizospheric microbes, pathogenicity test, <em>in vitro</em> and <em>in vivo</em> assays, growth promotion assays, and identification. There were 99 isolates of bacteria and 18 isolates of fungi isolated from the root and rhizosphere of rubber trees. <em>In vitro</em> and <em>in vivo</em> assay showed that 2 bacterial isolates, i.e. endophytic bacteria ME8, and rhizospheric bacteria MR3; and 3 fungal isolates, i.e. endophytic fungi CB8, CB6, and CL3 were able to inhibit the growth of <em>R. lignosus. </em>Endophytic bacteria ME8 showed the ability of solibilizing phosphate and fixing nitrogen. Rhizospheric bacteria MR3 showed the ability of solubilizing phosphate. The isolates CB6 and CL3 were very similar with<em> Chaetomium </em>sp. and <em>Penicillium </em>sp., respectively based on morphological characters; while CB8 was identified as mycelial sterile. Based on 16S rRNA sequences, endophytic bacterium ME8 and rhizospheric bacteria ME3 were identified as <em>Bacillus siamensis </em>B268 and <em>B. amylolyquefaciens </em>BCRh10, respectively. Endophytic and rhizospheric microbes isolated from rubber trees has the potency as biocontrol agents of <em>R. lignosus.</em></span> |
| first_indexed | 2024-12-11T07:29:36Z |
| format | Article |
| id | doaj.art-fba0c196c9284d7eb29ac71413abccba |
| institution | Directory Open Access Journal |
| issn | 0215-7950 2339-2479 |
| language | Indonesian |
| last_indexed | 2024-12-11T07:29:36Z |
| publishDate | 2018-01-01 |
| publisher | Perhimpunan Fitopatologi Indonesia |
| record_format | Article |
| series | Jurnal Fitopatologi Indonesia |
| spelling | doaj.art-fba0c196c9284d7eb29ac71413abccba2022-12-22T01:15:52ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792018-01-0113510.14692/jfi.13.5.17513115Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosusSiti Hardiyanti0Bonny Poernomo Wahyu Soekarno1Titiek Siti Yuliani2Institut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian Bogor<em><span lang="EN-US">Rigidoporus lignosus </span></em><span lang="EN-US">is the most important pathogen of rubber tree which causes white root rot disease. The use of antagonistic microbe is recommended to control this pathogen. This research was conducted to isolate endophytic and rhizospheric microbes, and to study their ability to inhibit growth of <em>R. lignosus</em>. Research consisted of isolation of endophytic and rhizospheric microbes, pathogenicity test, <em>in vitro</em> and <em>in vivo</em> assays, growth promotion assays, and identification. There were 99 isolates of bacteria and 18 isolates of fungi isolated from the root and rhizosphere of rubber trees. <em>In vitro</em> and <em>in vivo</em> assay showed that 2 bacterial isolates, i.e. endophytic bacteria ME8, and rhizospheric bacteria MR3; and 3 fungal isolates, i.e. endophytic fungi CB8, CB6, and CL3 were able to inhibit the growth of <em>R. lignosus. </em>Endophytic bacteria ME8 showed the ability of solibilizing phosphate and fixing nitrogen. Rhizospheric bacteria MR3 showed the ability of solubilizing phosphate. The isolates CB6 and CL3 were very similar with<em> Chaetomium </em>sp. and <em>Penicillium </em>sp., respectively based on morphological characters; while CB8 was identified as mycelial sterile. Based on 16S rRNA sequences, endophytic bacterium ME8 and rhizospheric bacteria ME3 were identified as <em>Bacillus siamensis </em>B268 and <em>B. amylolyquefaciens </em>BCRh10, respectively. Endophytic and rhizospheric microbes isolated from rubber trees has the potency as biocontrol agents of <em>R. lignosus.</em></span>http://journal.ipb.ac.id/index.php/jfiti/article/view/19632antagonistic microbe, nitrogen fixer, phosphate solubilizer, root rot disease |
| spellingShingle | Siti Hardiyanti Bonny Poernomo Wahyu Soekarno Titiek Siti Yuliani Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus Jurnal Fitopatologi Indonesia antagonistic microbe, nitrogen fixer, phosphate solubilizer, root rot disease |
| title | Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus |
| title_full | Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus |
| title_fullStr | Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus |
| title_full_unstemmed | Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus |
| title_short | Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus |
| title_sort | kemampuan mikrob endofit dan rizosfer tanaman karet dalam mengendalikan rigidoporus lignosus |
| topic | antagonistic microbe, nitrogen fixer, phosphate solubilizer, root rot disease |
| url | http://journal.ipb.ac.id/index.php/jfiti/article/view/19632 |
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