Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin
<i>Equisetum myriochaetum</i> is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus <i>Equisetum</i> stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent....
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/2/443 |
| _version_ | 1797410865310334976 |
|---|---|
| author | Fabián Olazarán-Santibañez Gildardo Rivera Venancio Vanoye-Eligio Arturo Mora-Olivo Gabriel Aguirre-Guzmán Mónica Ramírez-Cabrera Eder Arredondo-Espinoza |
| author_facet | Fabián Olazarán-Santibañez Gildardo Rivera Venancio Vanoye-Eligio Arturo Mora-Olivo Gabriel Aguirre-Guzmán Mónica Ramírez-Cabrera Eder Arredondo-Espinoza |
| author_sort | Fabián Olazarán-Santibañez |
| collection | DOAJ |
| description | <i>Equisetum myriochaetum</i> is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus <i>Equisetum</i> stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, <i>E. myriochaetum</i> ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of <i>E. myriochaetum</i> ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC<sub>50</sub>) value of 6.78 μg/mL against SiHa cells. |
| first_indexed | 2024-03-09T04:36:25Z |
| format | Article |
| id | doaj.art-fba1138a302248fdaa5a4a7545559e11 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:36:25Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fba1138a302248fdaa5a4a7545559e112023-12-03T13:28:17ZengMDPI AGMolecules1420-30492021-01-0126244310.3390/molecules26020443Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-TubulinFabián Olazarán-Santibañez0Gildardo Rivera1Venancio Vanoye-Eligio2Arturo Mora-Olivo3Gabriel Aguirre-Guzmán4Mónica Ramírez-Cabrera5Eder Arredondo-Espinoza6Instituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87019, MexicoCentro de Biotecnología Genómica, Laboratorio de Biotecnología Farmacéutica, Instituto Politécnico Nacional, Reynosa 88710, MexicoInstituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87019, MexicoInstituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87019, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87000, MexicoLaboratorio de Farmacología Molecular y Modelos Biológicos, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo Leon, Av Guerrero s/n, Treviño, C.P., Monterrey 64570, MexicoLaboratorio de Farmacología Molecular y Modelos Biológicos, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo Leon, Av Guerrero s/n, Treviño, C.P., Monterrey 64570, Mexico<i>Equisetum myriochaetum</i> is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus <i>Equisetum</i> stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, <i>E. myriochaetum</i> ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of <i>E. myriochaetum</i> ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC<sub>50</sub>) value of 6.78 μg/mL against SiHa cells.https://www.mdpi.com/1420-3049/26/2/443antioxidativeantiproliferative<i>Equisetum myriochaetum</i>flavonoidskaempferolmolecular docking |
| spellingShingle | Fabián Olazarán-Santibañez Gildardo Rivera Venancio Vanoye-Eligio Arturo Mora-Olivo Gabriel Aguirre-Guzmán Mónica Ramírez-Cabrera Eder Arredondo-Espinoza Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin Molecules antioxidative antiproliferative <i>Equisetum myriochaetum</i> flavonoids kaempferol molecular docking |
| title | Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin |
| title_full | Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin |
| title_fullStr | Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin |
| title_full_unstemmed | Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin |
| title_short | Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin |
| title_sort | antioxidant and antiproliferative activity of the ethanolic extract of i equisetum myriochaetum i and molecular docking of its main metabolites apigenin kaempferol and quercetin on β tubulin |
| topic | antioxidative antiproliferative <i>Equisetum myriochaetum</i> flavonoids kaempferol molecular docking |
| url | https://www.mdpi.com/1420-3049/26/2/443 |
| work_keys_str_mv | AT fabianolazaransantibanez antioxidantandantiproliferativeactivityoftheethanolicextractofiequisetummyriochaetumiandmoleculardockingofitsmainmetabolitesapigeninkaempferolandquercetinonbtubulin AT gildardorivera antioxidantandantiproliferativeactivityoftheethanolicextractofiequisetummyriochaetumiandmoleculardockingofitsmainmetabolitesapigeninkaempferolandquercetinonbtubulin AT venanciovanoyeeligio antioxidantandantiproliferativeactivityoftheethanolicextractofiequisetummyriochaetumiandmoleculardockingofitsmainmetabolitesapigeninkaempferolandquercetinonbtubulin AT arturomoraolivo antioxidantandantiproliferativeactivityoftheethanolicextractofiequisetummyriochaetumiandmoleculardockingofitsmainmetabolitesapigeninkaempferolandquercetinonbtubulin AT gabrielaguirreguzman antioxidantandantiproliferativeactivityoftheethanolicextractofiequisetummyriochaetumiandmoleculardockingofitsmainmetabolitesapigeninkaempferolandquercetinonbtubulin AT monicaramirezcabrera antioxidantandantiproliferativeactivityoftheethanolicextractofiequisetummyriochaetumiandmoleculardockingofitsmainmetabolitesapigeninkaempferolandquercetinonbtubulin AT ederarredondoespinoza antioxidantandantiproliferativeactivityoftheethanolicextractofiequisetummyriochaetumiandmoleculardockingofitsmainmetabolitesapigeninkaempferolandquercetinonbtubulin |