Generating Novel Tomato Germplasm Using the Ancestral Wild Relative of <i>Solanum pimpinellifolium</i>
To create novel tomato (<i>Solanum lycopersicum</i>) germplasm, a wild tomato relative, <i>S. pimpinellifolium</i> (a red-fruited LA1585 accession), was used as the male parent to cross with the yellow-fruited tomato mutant, <i>e9292</i> (<i>S. lycopersicum&...
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MDPI AG
2022-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/1/34 |
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| author | Wenzhen Li Yuhang Li Yingnuan Liang Linlin Ni Huofeng Huang Yushuang Wei Mingcui Wang Lida Zhang Lingxia Zhao |
| author_facet | Wenzhen Li Yuhang Li Yingnuan Liang Linlin Ni Huofeng Huang Yushuang Wei Mingcui Wang Lida Zhang Lingxia Zhao |
| author_sort | Wenzhen Li |
| collection | DOAJ |
| description | To create novel tomato (<i>Solanum lycopersicum</i>) germplasm, a wild tomato relative, <i>S. pimpinellifolium</i> (a red-fruited LA1585 accession), was used as the male parent to cross with the yellow-fruited tomato mutant, <i>e9292</i> (<i>S. lycopersicum</i>). Forty-four morphological characteristics were examined in the present study; 22 <i>S. pimpinellifolium</i> (LA1585) traits and six <i>S. lycopersicum</i> (<i>e9292</i>) traits were dominant in the hybrids (first generation, F<sub>1</sub>); 10 were intermediate types, and the remaining six resulted in a nonmorphological difference. Ten representative lines were chosen from 1338 line of the second generation of the hybrid (<i>e9292</i> × LA1585). The ascorbic acid content was higher in three F<sub>2</sub> hybrids than in LA1585, which had higher parental levels, as well as the lycopene content in two of the hybrid lines. The fructose and glucose contents were higher in five F<sub>2</sub> hybrids compared to <i>e9292</i>, and the sugar/acid value was higher in three hybrid lines. The broad-sense heritability values ranged from 75.06% for total soluble solids to 99.92% for ascorbic acid, and the average value was 92.66% for 15 quality traits. When seedlings were exposed to NaCl and mannitol, the tolerance of high salt concentrations and drought was enhanced in two hybrid lines (F<sub>2</sub>-266 and F<sub>2</sub>-299) compared with else tomatoes (<i>e9292</i>, LA1585, F<sub>1</sub> hybrids, and F<sub>2</sub>-332). We created novel tomato germplasm resources with horticulturally desirable quality traits and abiotic stress tolerance, thus offering a methodology for novel tomato germplasm creation and evaluation. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T12:31:15Z |
| publishDate | 2022-12-01 |
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| series | Horticulturae |
| spelling | doaj.art-fba1d06e20a346e2a9107443af2640d92023-11-30T22:29:57ZengMDPI AGHorticulturae2311-75242022-12-01913410.3390/horticulturae9010034Generating Novel Tomato Germplasm Using the Ancestral Wild Relative of <i>Solanum pimpinellifolium</i>Wenzhen Li0Yuhang Li1Yingnuan Liang2Linlin Ni3Huofeng Huang4Yushuang Wei5Mingcui Wang6Lida Zhang7Lingxia Zhao8Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaAgriculture Service Center, Kunshan 215300, ChinaAgriculture Service Center, Kunshan 215300, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaTo create novel tomato (<i>Solanum lycopersicum</i>) germplasm, a wild tomato relative, <i>S. pimpinellifolium</i> (a red-fruited LA1585 accession), was used as the male parent to cross with the yellow-fruited tomato mutant, <i>e9292</i> (<i>S. lycopersicum</i>). Forty-four morphological characteristics were examined in the present study; 22 <i>S. pimpinellifolium</i> (LA1585) traits and six <i>S. lycopersicum</i> (<i>e9292</i>) traits were dominant in the hybrids (first generation, F<sub>1</sub>); 10 were intermediate types, and the remaining six resulted in a nonmorphological difference. Ten representative lines were chosen from 1338 line of the second generation of the hybrid (<i>e9292</i> × LA1585). The ascorbic acid content was higher in three F<sub>2</sub> hybrids than in LA1585, which had higher parental levels, as well as the lycopene content in two of the hybrid lines. The fructose and glucose contents were higher in five F<sub>2</sub> hybrids compared to <i>e9292</i>, and the sugar/acid value was higher in three hybrid lines. The broad-sense heritability values ranged from 75.06% for total soluble solids to 99.92% for ascorbic acid, and the average value was 92.66% for 15 quality traits. When seedlings were exposed to NaCl and mannitol, the tolerance of high salt concentrations and drought was enhanced in two hybrid lines (F<sub>2</sub>-266 and F<sub>2</sub>-299) compared with else tomatoes (<i>e9292</i>, LA1585, F<sub>1</sub> hybrids, and F<sub>2</sub>-332). We created novel tomato germplasm resources with horticulturally desirable quality traits and abiotic stress tolerance, thus offering a methodology for novel tomato germplasm creation and evaluation.https://www.mdpi.com/2311-7524/9/1/34<i>Solanum lycopersicum</i><i>S. pimpinellifolium</i>genetic improvementquality traitsgermplasm resources |
| spellingShingle | Wenzhen Li Yuhang Li Yingnuan Liang Linlin Ni Huofeng Huang Yushuang Wei Mingcui Wang Lida Zhang Lingxia Zhao Generating Novel Tomato Germplasm Using the Ancestral Wild Relative of <i>Solanum pimpinellifolium</i> Horticulturae <i>Solanum lycopersicum</i> <i>S. pimpinellifolium</i> genetic improvement quality traits germplasm resources |
| title | Generating Novel Tomato Germplasm Using the Ancestral Wild Relative of <i>Solanum pimpinellifolium</i> |
| title_full | Generating Novel Tomato Germplasm Using the Ancestral Wild Relative of <i>Solanum pimpinellifolium</i> |
| title_fullStr | Generating Novel Tomato Germplasm Using the Ancestral Wild Relative of <i>Solanum pimpinellifolium</i> |
| title_full_unstemmed | Generating Novel Tomato Germplasm Using the Ancestral Wild Relative of <i>Solanum pimpinellifolium</i> |
| title_short | Generating Novel Tomato Germplasm Using the Ancestral Wild Relative of <i>Solanum pimpinellifolium</i> |
| title_sort | generating novel tomato germplasm using the ancestral wild relative of i solanum pimpinellifolium i |
| topic | <i>Solanum lycopersicum</i> <i>S. pimpinellifolium</i> genetic improvement quality traits germplasm resources |
| url | https://www.mdpi.com/2311-7524/9/1/34 |
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