Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent <i>Klebsiella pneumoniae</i> Strain 52145, and Identification of Its Functional Depolymerase
<i>Klebsiella pneumoniae</i> is among the leading bacteria that cause nosocomial infections. The capsule of this Gram-negative bacterium is a dominant virulence factor, with a prominent role in defense and biofilm formation. Bacteriophages, which are specific for one bacterial strain and...
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MDPI AG
2021-03-01
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| author | Botond Zsombor Pertics Alysia Cox Adrienn Nyúl Nóra Szamek Tamás Kovács György Schneider |
| author_facet | Botond Zsombor Pertics Alysia Cox Adrienn Nyúl Nóra Szamek Tamás Kovács György Schneider |
| author_sort | Botond Zsombor Pertics |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> is among the leading bacteria that cause nosocomial infections. The capsule of this Gram-negative bacterium is a dominant virulence factor, with a prominent role in defense and biofilm formation. Bacteriophages, which are specific for one bacterial strain and its capsule type, can evoke the lysis of bacterial cells, aided by polysaccharide depolymerase enzymes. In this study, we isolated and characterized a bacteriophage against the nosocomial <i>K. pneumoniae</i> 52145 strain with K2 capsular serotype. The phage showed a narrow host range and stable lytic activity, even when exposed to different temperatures or detergents. Preventive effect of the phage in a nasal colonization model was investigated in vivo. Phlyogenetic analysis showed that the newly isolated <i>Klebsiella</i> phage B1 belongs to the <i>Webervirus</i> genus in <i>Drexlerviridae</i> family. We identified the location of the capsule depolymerase gene of the new phage, which was amplified, cloned, expressed, and purified. The efficacy of the recombinant B1dep depolymerase was tested by spotting on <i>K. pneumoniae</i> strains and it was confirmed that the extract lowers the thickness of the bacterium lawn as it degrades the protective capsule on bacterial cells. As <i>K. pneumoniae</i> strains possessing the K2 serotype have epidemiological importance, the B1 phage and its depolymerase are promising candidates for use as possible antimicrobial agents. |
| first_indexed | 2024-03-10T13:03:26Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:03:26Z |
| publishDate | 2021-03-01 |
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| series | Microorganisms |
| spelling | doaj.art-fba2880f1a784736b0e2908e6495ac072023-11-21T11:23:44ZengMDPI AGMicroorganisms2076-26072021-03-019365010.3390/microorganisms9030650Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent <i>Klebsiella pneumoniae</i> Strain 52145, and Identification of Its Functional DepolymeraseBotond Zsombor Pertics0Alysia Cox1Adrienn Nyúl2Nóra Szamek3Tamás Kovács4György Schneider5Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti st. 12., H-7624 Pécs, HungaryDepartment of Biotechnology, Nanophagetherapy Center, Enviroinvest Corporation, Kertváros st. 2., H-7632 Pécs, HungaryDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti st. 12., H-7624 Pécs, HungaryDepartment of Biotechnology, Nanophagetherapy Center, Enviroinvest Corporation, Kertváros st. 2., H-7632 Pécs, HungaryDepartment of Biotechnology, Nanophagetherapy Center, Enviroinvest Corporation, Kertváros st. 2., H-7632 Pécs, HungaryDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti st. 12., H-7624 Pécs, Hungary<i>Klebsiella pneumoniae</i> is among the leading bacteria that cause nosocomial infections. The capsule of this Gram-negative bacterium is a dominant virulence factor, with a prominent role in defense and biofilm formation. Bacteriophages, which are specific for one bacterial strain and its capsule type, can evoke the lysis of bacterial cells, aided by polysaccharide depolymerase enzymes. In this study, we isolated and characterized a bacteriophage against the nosocomial <i>K. pneumoniae</i> 52145 strain with K2 capsular serotype. The phage showed a narrow host range and stable lytic activity, even when exposed to different temperatures or detergents. Preventive effect of the phage in a nasal colonization model was investigated in vivo. Phlyogenetic analysis showed that the newly isolated <i>Klebsiella</i> phage B1 belongs to the <i>Webervirus</i> genus in <i>Drexlerviridae</i> family. We identified the location of the capsule depolymerase gene of the new phage, which was amplified, cloned, expressed, and purified. The efficacy of the recombinant B1dep depolymerase was tested by spotting on <i>K. pneumoniae</i> strains and it was confirmed that the extract lowers the thickness of the bacterium lawn as it degrades the protective capsule on bacterial cells. As <i>K. pneumoniae</i> strains possessing the K2 serotype have epidemiological importance, the B1 phage and its depolymerase are promising candidates for use as possible antimicrobial agents.https://www.mdpi.com/2076-2607/9/3/650<i>Klebsiella pneumoniae</i>K2 serotypebacteriophagetail fiberscapsule depolymerase |
| spellingShingle | Botond Zsombor Pertics Alysia Cox Adrienn Nyúl Nóra Szamek Tamás Kovács György Schneider Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent <i>Klebsiella pneumoniae</i> Strain 52145, and Identification of Its Functional Depolymerase Microorganisms <i>Klebsiella pneumoniae</i> K2 serotype bacteriophage tail fibers capsule depolymerase |
| title | Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent <i>Klebsiella pneumoniae</i> Strain 52145, and Identification of Its Functional Depolymerase |
| title_full | Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent <i>Klebsiella pneumoniae</i> Strain 52145, and Identification of Its Functional Depolymerase |
| title_fullStr | Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent <i>Klebsiella pneumoniae</i> Strain 52145, and Identification of Its Functional Depolymerase |
| title_full_unstemmed | Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent <i>Klebsiella pneumoniae</i> Strain 52145, and Identification of Its Functional Depolymerase |
| title_short | Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent <i>Klebsiella pneumoniae</i> Strain 52145, and Identification of Its Functional Depolymerase |
| title_sort | isolation and characterization of a novel lytic bacteriophage against the k2 capsule expressing hypervirulent i klebsiella pneumoniae i strain 52145 and identification of its functional depolymerase |
| topic | <i>Klebsiella pneumoniae</i> K2 serotype bacteriophage tail fibers capsule depolymerase |
| url | https://www.mdpi.com/2076-2607/9/3/650 |
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