Development of Cryopreservation Technique for Meristems of <i>Syringa vulgaris</i> L. Cultivars
Cryopreservation is considered to be one of the most effective methods for long-term storage of plant genetic resources, particularly for ornamental species. However, there is a very little research on cryopreservation of lilacs. In this study, for the first time the cryopreservation protocol (a var...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | International Journal of Plant Biology |
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| Online Access: | https://www.mdpi.com/2037-0164/14/3/48 |
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| author | Olga Vasilevna Koroleva Olga Ivanovna Molkanova Olga Nikolaevna Vysotskaya |
| author_facet | Olga Vasilevna Koroleva Olga Ivanovna Molkanova Olga Nikolaevna Vysotskaya |
| author_sort | Olga Vasilevna Koroleva |
| collection | DOAJ |
| description | Cryopreservation is considered to be one of the most effective methods for long-term storage of plant genetic resources, particularly for ornamental species. However, there is a very little research on cryopreservation of lilacs. In this study, for the first time the cryopreservation protocol (a variation of a pregrowth-dehydration method) was successfully applied to two cultivars of <i>Syringa vulgaris</i>: ‘Aucubaefolia’ and ‘Polina Osipenko’. Explants of both cultivars were able to withstand the different steps of the protocol, and high survival and regrowth percentages were obtained after exposure to liquid nitrogen (67–100% and 63–88%, respectively). The current study is mainly focused on the preculture conditions of the applied method. Based on our results, we propose the use of paclobutrazol (PBZ) with the combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the preculture medium for increasing explant tolerance to subsequent dehydration and freezing. During post-LN recovery, the explants appeared morphologically normal, and after 12–16 weeks after thawing, they were propagated and cultured as normal plantlets. Therefore, the reported method is effective for long-term storage of lilac meristems and could be used to create a cryobank of achievements in lilac breeding. |
| first_indexed | 2024-03-10T22:39:51Z |
| format | Article |
| id | doaj.art-fba3903ce6314734b01e53298080dba1 |
| institution | Directory Open Access Journal |
| issn | 2037-0164 |
| language | English |
| last_indexed | 2024-03-10T22:39:51Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Plant Biology |
| spelling | doaj.art-fba3903ce6314734b01e53298080dba12023-11-19T11:12:37ZengMDPI AGInternational Journal of Plant Biology2037-01642023-07-0114362563710.3390/ijpb14030048Development of Cryopreservation Technique for Meristems of <i>Syringa vulgaris</i> L. CultivarsOlga Vasilevna Koroleva0Olga Ivanovna Molkanova1Olga Nikolaevna Vysotskaya2FSBIS Tsitsin Main Botanical Garden of Russian Academy of Science, 127276 Moscow, RussiaFSBIS Tsitsin Main Botanical Garden of Russian Academy of Science, 127276 Moscow, RussiaFSBIS Timiryazev Institute of Plant Physiology of Russian Academy of Science, 127276 Moscow, RussiaCryopreservation is considered to be one of the most effective methods for long-term storage of plant genetic resources, particularly for ornamental species. However, there is a very little research on cryopreservation of lilacs. In this study, for the first time the cryopreservation protocol (a variation of a pregrowth-dehydration method) was successfully applied to two cultivars of <i>Syringa vulgaris</i>: ‘Aucubaefolia’ and ‘Polina Osipenko’. Explants of both cultivars were able to withstand the different steps of the protocol, and high survival and regrowth percentages were obtained after exposure to liquid nitrogen (67–100% and 63–88%, respectively). The current study is mainly focused on the preculture conditions of the applied method. Based on our results, we propose the use of paclobutrazol (PBZ) with the combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the preculture medium for increasing explant tolerance to subsequent dehydration and freezing. During post-LN recovery, the explants appeared morphologically normal, and after 12–16 weeks after thawing, they were propagated and cultured as normal plantlets. Therefore, the reported method is effective for long-term storage of lilac meristems and could be used to create a cryobank of achievements in lilac breeding.https://www.mdpi.com/2037-0164/14/3/48lilaccryopreservationpreculture6-benzylaminopurinepaclobutrazolthidiazuron |
| spellingShingle | Olga Vasilevna Koroleva Olga Ivanovna Molkanova Olga Nikolaevna Vysotskaya Development of Cryopreservation Technique for Meristems of <i>Syringa vulgaris</i> L. Cultivars International Journal of Plant Biology lilac cryopreservation preculture 6-benzylaminopurine paclobutrazol thidiazuron |
| title | Development of Cryopreservation Technique for Meristems of <i>Syringa vulgaris</i> L. Cultivars |
| title_full | Development of Cryopreservation Technique for Meristems of <i>Syringa vulgaris</i> L. Cultivars |
| title_fullStr | Development of Cryopreservation Technique for Meristems of <i>Syringa vulgaris</i> L. Cultivars |
| title_full_unstemmed | Development of Cryopreservation Technique for Meristems of <i>Syringa vulgaris</i> L. Cultivars |
| title_short | Development of Cryopreservation Technique for Meristems of <i>Syringa vulgaris</i> L. Cultivars |
| title_sort | development of cryopreservation technique for meristems of i syringa vulgaris i l cultivars |
| topic | lilac cryopreservation preculture 6-benzylaminopurine paclobutrazol thidiazuron |
| url | https://www.mdpi.com/2037-0164/14/3/48 |
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