Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method
Brown patch caused by the basidiomycete fungus Rhizoctonia solani is an economically important disease of cool-season turfgrasses. In order to manage the disease, different types of fungicides have been applied, but the negative impact of fungicides on the environment continues to rise. In this stud...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1091030/full |
| _version_ | 1797956918528966656 |
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| author | Gahee Lee Hyeongju Choi Haifeng Liu Yun-Hyeong Han Yun-Hyeong Han Narayan Chandra Paul Narayan Chandra Paul Gui Hwan Han Hyunsook Kim Pyoung Il Kim Sun-Il Seo Jaekyeong Song Hyunkyu Sang Hyunkyu Sang Hyunkyu Sang |
| author_facet | Gahee Lee Hyeongju Choi Haifeng Liu Yun-Hyeong Han Yun-Hyeong Han Narayan Chandra Paul Narayan Chandra Paul Gui Hwan Han Hyunsook Kim Pyoung Il Kim Sun-Il Seo Jaekyeong Song Hyunkyu Sang Hyunkyu Sang Hyunkyu Sang |
| author_sort | Gahee Lee |
| collection | DOAJ |
| description | Brown patch caused by the basidiomycete fungus Rhizoctonia solani is an economically important disease of cool-season turfgrasses. In order to manage the disease, different types of fungicides have been applied, but the negative impact of fungicides on the environment continues to rise. In this study, the beneficial bacteria Bacillus velezensis GH1-13 was characterized as a potential biocontrol agent to manage brown patch disease. The strain GH1-13 strongly inhibited the mycelial growth of turf pathogens including different anastomosis groups of R. solani causing brown patch and large patch. R. solani AG2-2(IIIB) hyphae were morphologically changed, and fungal cell death resulted from exposure to the strain GH1-13. In addition, the compatibility of fungicides with the bacterial strain, and the combined application of fungicide azoxystrobin and the strain in brown patch control on creeping bentgrass indicated that the strain could serve as a biocontrol agent. To develop strain-specific detection method, two unique genes from chromosome and plasmid of GH1-13 were found using pan-genome analysis of 364 Bacillus strains. The unique gene from chromosome was successfully detected using both SYBR Green and TaqMan qPCR methods in bacterial DNA or soil DNA samples. This study suggests that application of GH1-13 offers an environmentally friendly approach via reducing fungicide application rates. Furthermore, the developed pipeline of strain-specific detection method could be a useful tool for detecting and studying the dynamics of specific biocontrol agents. |
| first_indexed | 2024-04-10T23:57:20Z |
| format | Article |
| id | doaj.art-fba67a4b780c40d0babb90cbf63e7779 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T23:57:20Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-fba67a4b780c40d0babb90cbf63e77792023-01-10T12:37:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10910301091030Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection methodGahee Lee0Hyeongju Choi1Haifeng Liu2Yun-Hyeong Han3Yun-Hyeong Han4Narayan Chandra Paul5Narayan Chandra Paul6Gui Hwan Han7Hyunsook Kim8Pyoung Il Kim9Sun-Il Seo10Jaekyeong Song11Hyunkyu Sang12Hyunkyu Sang13Hyunkyu Sang14Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, Republic of KoreaDepartment of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, Republic of KoreaDepartment of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, Republic of KoreaDivision of Food and Biotechnology, Chonnam National University, Gwangju, Republic of KoreaDamyang-gun Agricultural Technology Center, Damyang, Republic of KoreaDepartment of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, Republic of KoreaKumho Life Science Laboratory, Chonnam National University, Gwangju, Republic of KoreaCenter for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup, Republic of KoreaBoran Pharma, Seoul, Republic of KoreaCenter for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup, Republic of KoreaCenter for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup, Republic of KoreaAgricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Republic of KoreaDepartment of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, Republic of KoreaDivision of Food and Biotechnology, Chonnam National University, Gwangju, Republic of KoreaKumho Life Science Laboratory, Chonnam National University, Gwangju, Republic of KoreaBrown patch caused by the basidiomycete fungus Rhizoctonia solani is an economically important disease of cool-season turfgrasses. In order to manage the disease, different types of fungicides have been applied, but the negative impact of fungicides on the environment continues to rise. In this study, the beneficial bacteria Bacillus velezensis GH1-13 was characterized as a potential biocontrol agent to manage brown patch disease. The strain GH1-13 strongly inhibited the mycelial growth of turf pathogens including different anastomosis groups of R. solani causing brown patch and large patch. R. solani AG2-2(IIIB) hyphae were morphologically changed, and fungal cell death resulted from exposure to the strain GH1-13. In addition, the compatibility of fungicides with the bacterial strain, and the combined application of fungicide azoxystrobin and the strain in brown patch control on creeping bentgrass indicated that the strain could serve as a biocontrol agent. To develop strain-specific detection method, two unique genes from chromosome and plasmid of GH1-13 were found using pan-genome analysis of 364 Bacillus strains. The unique gene from chromosome was successfully detected using both SYBR Green and TaqMan qPCR methods in bacterial DNA or soil DNA samples. This study suggests that application of GH1-13 offers an environmentally friendly approach via reducing fungicide application rates. Furthermore, the developed pipeline of strain-specific detection method could be a useful tool for detecting and studying the dynamics of specific biocontrol agents.https://www.frontiersin.org/articles/10.3389/fpls.2022.1091030/fullBacillus velezensisbiocontrolbrown patchpan-genomedetectionRhizoctonia solani |
| spellingShingle | Gahee Lee Hyeongju Choi Haifeng Liu Yun-Hyeong Han Yun-Hyeong Han Narayan Chandra Paul Narayan Chandra Paul Gui Hwan Han Hyunsook Kim Pyoung Il Kim Sun-Il Seo Jaekyeong Song Hyunkyu Sang Hyunkyu Sang Hyunkyu Sang Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method Frontiers in Plant Science Bacillus velezensis biocontrol brown patch pan-genome detection Rhizoctonia solani |
| title | Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method |
| title_full | Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method |
| title_fullStr | Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method |
| title_full_unstemmed | Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method |
| title_short | Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method |
| title_sort | biocontrol of the causal brown patch pathogen rhizoctonia solani by bacillus velezensis gh1 13 and development of a bacterial strain specific detection method |
| topic | Bacillus velezensis biocontrol brown patch pan-genome detection Rhizoctonia solani |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1091030/full |
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