Homologs of bacterial heat-labile enterotoxin subunit A contribute to development, stress response, and virulence in filamentous entomopathogenic fungus Beauveria bassiana
IntroductionEnterotoxigenic bacteria commonly excrete heat-labile enterotoxins (LT) as virulence factors that consist of one subunit A (LTA) and five B subunits (LTB). In fungi, there are a large number of genes encoding the homologs of LTA, but their biological roles remain largely unknown. Methods...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-09-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1264560/full |
| _version_ | 1827809018416988160 |
|---|---|
| author | Jin-Li Ding Kang Wei Ming-Guang Feng Sheng-Hua Ying |
| author_facet | Jin-Li Ding Kang Wei Ming-Guang Feng Sheng-Hua Ying |
| author_sort | Jin-Li Ding |
| collection | DOAJ |
| description | IntroductionEnterotoxigenic bacteria commonly excrete heat-labile enterotoxins (LT) as virulence factors that consist of one subunit A (LTA) and five B subunits (LTB). In fungi, there are a large number of genes encoding the homologs of LTA, but their biological roles remain largely unknown. MethodsIn this study, we identified 14 enterotoxin_A domain proteins in filamentous fungus B. bassiana in which five proteins were functionally characterized. ResultsFive proteins displayed diverse sub-cellular localizations but perform convergent functions in stress response, development, and virulence. The loss of five LTA genes resulted in significant reduction in conidial production, blastospore formation, and the increased sensitivity to oxidative and cell wall –perturbing stresses. The virulence of five disruptants was notably weakened as indicated by topical and intrahemocoel injection assays. Notably, the loss of these five proteins led to the significant changes in the carbohydrate profiles of cellular surface, which induced the enhanced host immune reactions of encapsulation and melanization. DiscussionThus, LTA proteins contribute to the fungus–host interaction via maintaining the carbohydrate profiles of cellular surface. This study expands our understanding of the enterotoxin_A domain proteins in fungal physiology and deepens mechanisms involved in the lifestyle of fungal insect pathogens. |
| first_indexed | 2024-03-11T22:37:28Z |
| format | Article |
| id | doaj.art-fbaaa67680e94eeab61c59e6097f8bb0 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T22:37:28Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-fbaaa67680e94eeab61c59e6097f8bb02023-09-22T13:04:16ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-09-011410.3389/fimmu.2023.12645601264560Homologs of bacterial heat-labile enterotoxin subunit A contribute to development, stress response, and virulence in filamentous entomopathogenic fungus Beauveria bassianaJin-Li DingKang WeiMing-Guang FengSheng-Hua YingIntroductionEnterotoxigenic bacteria commonly excrete heat-labile enterotoxins (LT) as virulence factors that consist of one subunit A (LTA) and five B subunits (LTB). In fungi, there are a large number of genes encoding the homologs of LTA, but their biological roles remain largely unknown. MethodsIn this study, we identified 14 enterotoxin_A domain proteins in filamentous fungus B. bassiana in which five proteins were functionally characterized. ResultsFive proteins displayed diverse sub-cellular localizations but perform convergent functions in stress response, development, and virulence. The loss of five LTA genes resulted in significant reduction in conidial production, blastospore formation, and the increased sensitivity to oxidative and cell wall –perturbing stresses. The virulence of five disruptants was notably weakened as indicated by topical and intrahemocoel injection assays. Notably, the loss of these five proteins led to the significant changes in the carbohydrate profiles of cellular surface, which induced the enhanced host immune reactions of encapsulation and melanization. DiscussionThus, LTA proteins contribute to the fungus–host interaction via maintaining the carbohydrate profiles of cellular surface. This study expands our understanding of the enterotoxin_A domain proteins in fungal physiology and deepens mechanisms involved in the lifestyle of fungal insect pathogens.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1264560/fullBeauveria bassianaenterotoxindevelopmentvirulencestress response |
| spellingShingle | Jin-Li Ding Kang Wei Ming-Guang Feng Sheng-Hua Ying Homologs of bacterial heat-labile enterotoxin subunit A contribute to development, stress response, and virulence in filamentous entomopathogenic fungus Beauveria bassiana Frontiers in Immunology Beauveria bassiana enterotoxin development virulence stress response |
| title | Homologs of bacterial heat-labile enterotoxin subunit A contribute to development, stress response, and virulence in filamentous entomopathogenic fungus Beauveria bassiana |
| title_full | Homologs of bacterial heat-labile enterotoxin subunit A contribute to development, stress response, and virulence in filamentous entomopathogenic fungus Beauveria bassiana |
| title_fullStr | Homologs of bacterial heat-labile enterotoxin subunit A contribute to development, stress response, and virulence in filamentous entomopathogenic fungus Beauveria bassiana |
| title_full_unstemmed | Homologs of bacterial heat-labile enterotoxin subunit A contribute to development, stress response, and virulence in filamentous entomopathogenic fungus Beauveria bassiana |
| title_short | Homologs of bacterial heat-labile enterotoxin subunit A contribute to development, stress response, and virulence in filamentous entomopathogenic fungus Beauveria bassiana |
| title_sort | homologs of bacterial heat labile enterotoxin subunit a contribute to development stress response and virulence in filamentous entomopathogenic fungus beauveria bassiana |
| topic | Beauveria bassiana enterotoxin development virulence stress response |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1264560/full |
| work_keys_str_mv | AT jinliding homologsofbacterialheatlabileenterotoxinsubunitacontributetodevelopmentstressresponseandvirulenceinfilamentousentomopathogenicfungusbeauveriabassiana AT kangwei homologsofbacterialheatlabileenterotoxinsubunitacontributetodevelopmentstressresponseandvirulenceinfilamentousentomopathogenicfungusbeauveriabassiana AT mingguangfeng homologsofbacterialheatlabileenterotoxinsubunitacontributetodevelopmentstressresponseandvirulenceinfilamentousentomopathogenicfungusbeauveriabassiana AT shenghuaying homologsofbacterialheatlabileenterotoxinsubunitacontributetodevelopmentstressresponseandvirulenceinfilamentousentomopathogenicfungusbeauveriabassiana |