Comparative Genomics and Physiological Characterization of Two Aerobic Spore Formers Isolated from Human Ileal Samples
Spore formers are ubiquitous microorganisms commonly isolated from most environments, including the gastro-intestinal tract (GIT) of insects and animals. Spores ingested as food and water contaminants safely transit the stomach and reach the intestine, where some of them germinate and temporarily co...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/23/14946 |
| _version_ | 1797463114431594496 |
|---|---|
| author | Anella Saggese Rosa Giglio Nicola D’Anzi Loredana Baccigalupi Ezio Ricca |
| author_facet | Anella Saggese Rosa Giglio Nicola D’Anzi Loredana Baccigalupi Ezio Ricca |
| author_sort | Anella Saggese |
| collection | DOAJ |
| description | Spore formers are ubiquitous microorganisms commonly isolated from most environments, including the gastro-intestinal tract (GIT) of insects and animals. Spores ingested as food and water contaminants safely transit the stomach and reach the intestine, where some of them germinate and temporarily colonize that niche. In the lower part of the GIT, they re-sporulate and leave the body as spores, therefore passing through their entire life cycle in the animal body. In the intestine, both un-germinated spores and germination-derived cells interact with intestinal and immune cells and have health-beneficial effects, which include the production of useful compounds, protection against pathogenic microorganisms, contribution to the development of an efficient immune system and modulation of the gut microbial composition. We report a genomic and physiological characterization of SF106 and SF174, two aerobic spore former strains previously isolated from ileal biopsies of healthy human volunteers. SF106 and SF174 belong respectively to the <i>B. subtilis</i> and <i>Alkalihalobacillus clausii</i> (formerly <i>Bacillus clausii</i>) species, are unable to produce toxins or other metabolites with cytotoxic activity against cultured human cells, efficiently bind mucin and human epithelial cells in vitro and produce molecules with antimicrobial and antibiofilm activities. |
| first_indexed | 2024-03-09T17:46:03Z |
| format | Article |
| id | doaj.art-fbb00f0bcdfc4c6788a6c9efd9cff58d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:46:03Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fbb00f0bcdfc4c6788a6c9efd9cff58d2023-11-24T11:10:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231494610.3390/ijms232314946Comparative Genomics and Physiological Characterization of Two Aerobic Spore Formers Isolated from Human Ileal SamplesAnella Saggese0Rosa Giglio1Nicola D’Anzi2Loredana Baccigalupi3Ezio Ricca4Department of Biology, Federico II University of Naples, 80125 Naples, ItalyGruppo Savio, Pomezia, 00071 Rome, ItalyGruppo Savio, Pomezia, 00071 Rome, ItalyDepartment of Molecular Medicine and Medical Biotechnology, Federico II University of Naples, 80131 Naples, ItalyDepartment of Biology, Federico II University of Naples, 80125 Naples, ItalySpore formers are ubiquitous microorganisms commonly isolated from most environments, including the gastro-intestinal tract (GIT) of insects and animals. Spores ingested as food and water contaminants safely transit the stomach and reach the intestine, where some of them germinate and temporarily colonize that niche. In the lower part of the GIT, they re-sporulate and leave the body as spores, therefore passing through their entire life cycle in the animal body. In the intestine, both un-germinated spores and germination-derived cells interact with intestinal and immune cells and have health-beneficial effects, which include the production of useful compounds, protection against pathogenic microorganisms, contribution to the development of an efficient immune system and modulation of the gut microbial composition. We report a genomic and physiological characterization of SF106 and SF174, two aerobic spore former strains previously isolated from ileal biopsies of healthy human volunteers. SF106 and SF174 belong respectively to the <i>B. subtilis</i> and <i>Alkalihalobacillus clausii</i> (formerly <i>Bacillus clausii</i>) species, are unable to produce toxins or other metabolites with cytotoxic activity against cultured human cells, efficiently bind mucin and human epithelial cells in vitro and produce molecules with antimicrobial and antibiofilm activities.https://www.mdpi.com/1422-0067/23/23/14946<i>Bacillus subtilis</i><i>Alkalihalobacillus clausii</i>antibiotic resistanceprobioticsbeneficial bacteriaantimicrobials |
| spellingShingle | Anella Saggese Rosa Giglio Nicola D’Anzi Loredana Baccigalupi Ezio Ricca Comparative Genomics and Physiological Characterization of Two Aerobic Spore Formers Isolated from Human Ileal Samples International Journal of Molecular Sciences <i>Bacillus subtilis</i> <i>Alkalihalobacillus clausii</i> antibiotic resistance probiotics beneficial bacteria antimicrobials |
| title | Comparative Genomics and Physiological Characterization of Two Aerobic Spore Formers Isolated from Human Ileal Samples |
| title_full | Comparative Genomics and Physiological Characterization of Two Aerobic Spore Formers Isolated from Human Ileal Samples |
| title_fullStr | Comparative Genomics and Physiological Characterization of Two Aerobic Spore Formers Isolated from Human Ileal Samples |
| title_full_unstemmed | Comparative Genomics and Physiological Characterization of Two Aerobic Spore Formers Isolated from Human Ileal Samples |
| title_short | Comparative Genomics and Physiological Characterization of Two Aerobic Spore Formers Isolated from Human Ileal Samples |
| title_sort | comparative genomics and physiological characterization of two aerobic spore formers isolated from human ileal samples |
| topic | <i>Bacillus subtilis</i> <i>Alkalihalobacillus clausii</i> antibiotic resistance probiotics beneficial bacteria antimicrobials |
| url | https://www.mdpi.com/1422-0067/23/23/14946 |
| work_keys_str_mv | AT anellasaggese comparativegenomicsandphysiologicalcharacterizationoftwoaerobicsporeformersisolatedfromhumanilealsamples AT rosagiglio comparativegenomicsandphysiologicalcharacterizationoftwoaerobicsporeformersisolatedfromhumanilealsamples AT nicoladanzi comparativegenomicsandphysiologicalcharacterizationoftwoaerobicsporeformersisolatedfromhumanilealsamples AT loredanabaccigalupi comparativegenomicsandphysiologicalcharacterizationoftwoaerobicsporeformersisolatedfromhumanilealsamples AT ezioricca comparativegenomicsandphysiologicalcharacterizationoftwoaerobicsporeformersisolatedfromhumanilealsamples |