Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In Vivo
The emergence of drug-resistant bacteria emphasizes the urgent need for novel antibiotics. The antimicrobial peptide TS shows extensive antibacterial activity in vitro and in vivo, especially in gram-negative bacteria; however, its antibacterial mechanism is unclear. Here, we find that TS without he...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/1/60 |
| _version_ | 1797543604800978944 |
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| author | Rui Zhang Xiaobo Fan Xinglu Jiang Mingyuan Zou Han Xiao Guoqiu Wu |
| author_facet | Rui Zhang Xiaobo Fan Xinglu Jiang Mingyuan Zou Han Xiao Guoqiu Wu |
| author_sort | Rui Zhang |
| collection | DOAJ |
| description | The emergence of drug-resistant bacteria emphasizes the urgent need for novel antibiotics. The antimicrobial peptide TS shows extensive antibacterial activity in vitro and in vivo, especially in gram-negative bacteria; however, its antibacterial mechanism is unclear. Here, we find that TS without hemolytic activity disrupts the integrity of the outer bacterial cell membrane by displacing divalent cations and competitively binding lipopolysaccharides. In addition, the antimicrobial peptide TS can inhibit and kill <i>E. coli</i> by disintegrating the bacteria from within by interacting with bacterial DNA. Thus, antimicrobial peptide TS’s multiple antibacterial mechanisms may not easily induce bacterial resistance, suggesting use as an antibacterial drug to be for combating bacterial infections in the future. |
| first_indexed | 2024-03-10T13:47:59Z |
| format | Article |
| id | doaj.art-fbb06194aa9e46d7aa344efae2bba64c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:47:59Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fbb06194aa9e46d7aa344efae2bba64c2023-11-21T02:30:24ZengMDPI AGMolecules1420-30492020-12-012616010.3390/molecules26010060Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In VivoRui Zhang0Xiaobo Fan1Xinglu Jiang2Mingyuan Zou3Han Xiao4Guoqiu Wu5Medical School, Southeast University, Nanjing 210009, ChinaMedical School, Southeast University, Nanjing 210009, ChinaMedical School, Southeast University, Nanjing 210009, ChinaMedical School, Southeast University, Nanjing 210009, ChinaMedical School, Southeast University, Nanjing 210009, ChinaMedical School, Southeast University, Nanjing 210009, ChinaThe emergence of drug-resistant bacteria emphasizes the urgent need for novel antibiotics. The antimicrobial peptide TS shows extensive antibacterial activity in vitro and in vivo, especially in gram-negative bacteria; however, its antibacterial mechanism is unclear. Here, we find that TS without hemolytic activity disrupts the integrity of the outer bacterial cell membrane by displacing divalent cations and competitively binding lipopolysaccharides. In addition, the antimicrobial peptide TS can inhibit and kill <i>E. coli</i> by disintegrating the bacteria from within by interacting with bacterial DNA. Thus, antimicrobial peptide TS’s multiple antibacterial mechanisms may not easily induce bacterial resistance, suggesting use as an antibacterial drug to be for combating bacterial infections in the future.https://www.mdpi.com/1420-3049/26/1/60antibacterial activitymembrane interactionlipopolysaccharidebacterial DNA |
| spellingShingle | Rui Zhang Xiaobo Fan Xinglu Jiang Mingyuan Zou Han Xiao Guoqiu Wu Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In Vivo Molecules antibacterial activity membrane interaction lipopolysaccharide bacterial DNA |
| title | Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In Vivo |
| title_full | Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In Vivo |
| title_fullStr | Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In Vivo |
| title_full_unstemmed | Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In Vivo |
| title_short | Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In Vivo |
| title_sort | multiple mechanisms of the synthesized antimicrobial peptide ts against gram negative bacteria for high efficacy antibacterial action in vivo |
| topic | antibacterial activity membrane interaction lipopolysaccharide bacterial DNA |
| url | https://www.mdpi.com/1420-3049/26/1/60 |
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