Structural insight on assembly-line catalysis in terpene biosynthesis
Substrate channeling can improve biosynthetic efficiency and has been implicated in the reactions of fusicoccadiene synthase. Here, the authors analyze this bifunctional enzyme complex by cryoEM, cross-linking MS and integrative modeling, providing structural insights into how substrate channeling i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-23589-9 |
| _version_ | 1818736214248783872 |
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| author | Jacque L. Faylo Trevor van Eeuwen Hee Jong Kim Jose J. Gorbea Colón Benjamin A. Garcia Kenji Murakami David W. Christianson |
| author_facet | Jacque L. Faylo Trevor van Eeuwen Hee Jong Kim Jose J. Gorbea Colón Benjamin A. Garcia Kenji Murakami David W. Christianson |
| author_sort | Jacque L. Faylo |
| collection | DOAJ |
| description | Substrate channeling can improve biosynthetic efficiency and has been implicated in the reactions of fusicoccadiene synthase. Here, the authors analyze this bifunctional enzyme complex by cryoEM, cross-linking MS and integrative modeling, providing structural insights into how substrate channeling is achieved. |
| first_indexed | 2024-12-18T00:33:35Z |
| format | Article |
| id | doaj.art-fbb2f4b36b8d4e3eaddd473f28e72330 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-18T00:33:35Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-fbb2f4b36b8d4e3eaddd473f28e723302022-12-21T21:27:05ZengNature PortfolioNature Communications2041-17232021-06-0112111210.1038/s41467-021-23589-9Structural insight on assembly-line catalysis in terpene biosynthesisJacque L. Faylo0Trevor van Eeuwen1Hee Jong Kim2Jose J. Gorbea Colón3Benjamin A. Garcia4Kenji Murakami5David W. Christianson6Roy and Diana Vagelos Laboratories, Department of Chemistry, University of PennsylvaniaDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of PennsylvaniaDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of PennsylvaniaDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of PennsylvaniaDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of PennsylvaniaDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of PennsylvaniaRoy and Diana Vagelos Laboratories, Department of Chemistry, University of PennsylvaniaSubstrate channeling can improve biosynthetic efficiency and has been implicated in the reactions of fusicoccadiene synthase. Here, the authors analyze this bifunctional enzyme complex by cryoEM, cross-linking MS and integrative modeling, providing structural insights into how substrate channeling is achieved.https://doi.org/10.1038/s41467-021-23589-9 |
| spellingShingle | Jacque L. Faylo Trevor van Eeuwen Hee Jong Kim Jose J. Gorbea Colón Benjamin A. Garcia Kenji Murakami David W. Christianson Structural insight on assembly-line catalysis in terpene biosynthesis Nature Communications |
| title | Structural insight on assembly-line catalysis in terpene biosynthesis |
| title_full | Structural insight on assembly-line catalysis in terpene biosynthesis |
| title_fullStr | Structural insight on assembly-line catalysis in terpene biosynthesis |
| title_full_unstemmed | Structural insight on assembly-line catalysis in terpene biosynthesis |
| title_short | Structural insight on assembly-line catalysis in terpene biosynthesis |
| title_sort | structural insight on assembly line catalysis in terpene biosynthesis |
| url | https://doi.org/10.1038/s41467-021-23589-9 |
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