Sportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assay
Abstract Sportomics is a subject-centered holistic method similar to metabolomics focusing on sports as the metabolic challenge. Dried blood spot is emerging as a technique due to its simplicity and reproducibility. In addition, mass spectrometry and integrative computational biology enhance our abi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-23300-y |
| _version_ | 1828094105511526400 |
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| author | Adriana Bassini Silvia Sartoretto Lukas Jurisica Alexandre Magno-França Leigh Anderson Terry Pearson Morty Razavi Vinod Chandran LeRoy Martin Igor Jurisica L. C. Cameron |
| author_facet | Adriana Bassini Silvia Sartoretto Lukas Jurisica Alexandre Magno-França Leigh Anderson Terry Pearson Morty Razavi Vinod Chandran LeRoy Martin Igor Jurisica L. C. Cameron |
| author_sort | Adriana Bassini |
| collection | DOAJ |
| description | Abstract Sportomics is a subject-centered holistic method similar to metabolomics focusing on sports as the metabolic challenge. Dried blood spot is emerging as a technique due to its simplicity and reproducibility. In addition, mass spectrometry and integrative computational biology enhance our ability to understand exercise-induced modifications. We studied inflammatory blood proteins (Alpha-1-acid glycoprotein—A1AG1; Albumin; Cystatin C; C-reactive protein—CRP; Hemoglobin—HBA; Haptoglobin—HPT; Insulin-like growth factor 1; Lipopolysaccharide binding protein—LBP; Mannose-binding lectin—MBL2; Myeloperoxidase—PERM and Serum amyloid A1—SAA1), in 687 samples from 97 World-class and Olympic athletes across 16 sports in nine states. Data were analyzed with Spearman's rank-order correlation. Major correlations with CRP, LBP; MBL2; A1AG1, and SAA1 were found. The pairs CRP-SAA1 and CRP-LBP appeared with a robust positive correlation. Other pairs, LBP-SAA1; A1AG1-CRP; A1AG1-SAA1; A1AG1-MBL, and A1AG1-LBP, showed a broader correlation across the sports. The protein–protein interaction map revealed 1500 interactions with 44 core proteins, 30 of them linked to immune system processing. We propose that the inflammation follow-up in exercise can provide knowledge for internal cargo management in training, competition, recovery, doping control, and a deeper understanding of health and disease. |
| first_indexed | 2024-04-11T06:55:11Z |
| format | Article |
| id | doaj.art-fbb34af587564787a1995d14e520605d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T06:55:11Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fbb34af587564787a1995d14e520605d2022-12-22T04:39:04ZengNature PortfolioScientific Reports2045-23222022-11-0112111210.1038/s41598-022-23300-ySportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assayAdriana Bassini0Silvia Sartoretto1Lukas Jurisica2Alexandre Magno-França3Leigh Anderson4Terry Pearson5Morty Razavi6Vinod Chandran7LeRoy Martin8Igor Jurisica9L. C. Cameron10Laboratory of Protein Biochemistry, Federal University of State of Rio de JaneiroLaboratory of Protein Biochemistry, Federal University of State of Rio de JaneiroLaboratory of Protein Biochemistry, Federal University of State of Rio de JaneiroLaboratory of Protein Biochemistry, Federal University of State of Rio de JaneiroSISCAPA Assay Technologies, Inc.SISCAPA Assay Technologies, Inc.SISCAPA Assay Technologies, Inc.Arthritis Program, Schroeder Arthritis Institute, Krembil Research Institute, University Health NetworkWaters TechnologiesOsteoarthritis Research Program, Division of Orthopedic Surgery, Schroeder Arthritis Institute, and Data Science Discovery Centre for Chronic Diseases, Krembil Research Institute, University Health NetworkLaboratory of Protein Biochemistry, Federal University of State of Rio de JaneiroAbstract Sportomics is a subject-centered holistic method similar to metabolomics focusing on sports as the metabolic challenge. Dried blood spot is emerging as a technique due to its simplicity and reproducibility. In addition, mass spectrometry and integrative computational biology enhance our ability to understand exercise-induced modifications. We studied inflammatory blood proteins (Alpha-1-acid glycoprotein—A1AG1; Albumin; Cystatin C; C-reactive protein—CRP; Hemoglobin—HBA; Haptoglobin—HPT; Insulin-like growth factor 1; Lipopolysaccharide binding protein—LBP; Mannose-binding lectin—MBL2; Myeloperoxidase—PERM and Serum amyloid A1—SAA1), in 687 samples from 97 World-class and Olympic athletes across 16 sports in nine states. Data were analyzed with Spearman's rank-order correlation. Major correlations with CRP, LBP; MBL2; A1AG1, and SAA1 were found. The pairs CRP-SAA1 and CRP-LBP appeared with a robust positive correlation. Other pairs, LBP-SAA1; A1AG1-CRP; A1AG1-SAA1; A1AG1-MBL, and A1AG1-LBP, showed a broader correlation across the sports. The protein–protein interaction map revealed 1500 interactions with 44 core proteins, 30 of them linked to immune system processing. We propose that the inflammation follow-up in exercise can provide knowledge for internal cargo management in training, competition, recovery, doping control, and a deeper understanding of health and disease.https://doi.org/10.1038/s41598-022-23300-y |
| spellingShingle | Adriana Bassini Silvia Sartoretto Lukas Jurisica Alexandre Magno-França Leigh Anderson Terry Pearson Morty Razavi Vinod Chandran LeRoy Martin Igor Jurisica L. C. Cameron Sportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assay Scientific Reports |
| title | Sportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assay |
| title_full | Sportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assay |
| title_fullStr | Sportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assay |
| title_full_unstemmed | Sportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assay |
| title_short | Sportomics method to assess acute phase proteins in Olympic level athletes using dried blood spots and multiplex assay |
| title_sort | sportomics method to assess acute phase proteins in olympic level athletes using dried blood spots and multiplex assay |
| url | https://doi.org/10.1038/s41598-022-23300-y |
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