A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers.
The sea lamprey is an important model organism for investigating the evolutionary origins of vertebrates. As more vertebrate genome sequences are obtained, evolutionary developmental biologists are becoming increasingly able to identify putative gene regulatory elements across the breadth of the ver...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3887057?pdf=render |
| _version_ | 1819087016850096128 |
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| author | Hugo J Parker Tatjana Sauka-Spengler Marianne Bronner Greg Elgar |
| author_facet | Hugo J Parker Tatjana Sauka-Spengler Marianne Bronner Greg Elgar |
| author_sort | Hugo J Parker |
| collection | DOAJ |
| description | The sea lamprey is an important model organism for investigating the evolutionary origins of vertebrates. As more vertebrate genome sequences are obtained, evolutionary developmental biologists are becoming increasingly able to identify putative gene regulatory elements across the breadth of the vertebrate taxa. The identification of these regions makes it possible to address how changes at the genomic level have led to changes in developmental gene regulatory networks and ultimately to the evolution of morphological diversity. Comparative genomics approaches using sea lamprey have already predicted a number of such regulatory elements in the lamprey genome. Functional characterisation of these sequences and other similar elements requires efficient reporter assays in lamprey. In this report, we describe the development of a transient transgenesis method for lamprey embryos. Focusing on conserved non-coding elements (CNEs), we use this method to investigate their functional conservation across the vertebrate subphylum. We find instances of both functional conservation and lineage-specific functional evolution of CNEs across vertebrates, emphasising the utility of functionally testing homologous CNEs in their host species. |
| first_indexed | 2024-12-21T21:29:27Z |
| format | Article |
| id | doaj.art-fbb49f74215b4992bd01610ecb84c48e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T21:29:27Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fbb49f74215b4992bd01610ecb84c48e2022-12-21T18:49:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8549210.1371/journal.pone.0085492A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers.Hugo J ParkerTatjana Sauka-SpenglerMarianne BronnerGreg ElgarThe sea lamprey is an important model organism for investigating the evolutionary origins of vertebrates. As more vertebrate genome sequences are obtained, evolutionary developmental biologists are becoming increasingly able to identify putative gene regulatory elements across the breadth of the vertebrate taxa. The identification of these regions makes it possible to address how changes at the genomic level have led to changes in developmental gene regulatory networks and ultimately to the evolution of morphological diversity. Comparative genomics approaches using sea lamprey have already predicted a number of such regulatory elements in the lamprey genome. Functional characterisation of these sequences and other similar elements requires efficient reporter assays in lamprey. In this report, we describe the development of a transient transgenesis method for lamprey embryos. Focusing on conserved non-coding elements (CNEs), we use this method to investigate their functional conservation across the vertebrate subphylum. We find instances of both functional conservation and lineage-specific functional evolution of CNEs across vertebrates, emphasising the utility of functionally testing homologous CNEs in their host species.http://europepmc.org/articles/PMC3887057?pdf=render |
| spellingShingle | Hugo J Parker Tatjana Sauka-Spengler Marianne Bronner Greg Elgar A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers. PLoS ONE |
| title | A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers. |
| title_full | A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers. |
| title_fullStr | A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers. |
| title_full_unstemmed | A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers. |
| title_short | A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers. |
| title_sort | reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers |
| url | http://europepmc.org/articles/PMC3887057?pdf=render |
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