Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement
Spatial cell organization and biofabrication of microcapillary networks in vitro has a great potential in tissue engineering and regenerative medicine. This study explores the impact of local cell density enhancement achieved through an innovative sound-based patterning on microcapillary networks fo...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Biomaterials and Biosystems |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666534424000072 |
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| author | N. Di Marzio R. Tognato E. Della Bella V. De Giorgis M. Manfredi A. Cochis M. Alini T. Serra |
| author_facet | N. Di Marzio R. Tognato E. Della Bella V. De Giorgis M. Manfredi A. Cochis M. Alini T. Serra |
| author_sort | N. Di Marzio |
| collection | DOAJ |
| description | Spatial cell organization and biofabrication of microcapillary networks in vitro has a great potential in tissue engineering and regenerative medicine. This study explores the impact of local cell density enhancement achieved through an innovative sound-based patterning on microcapillary networks formation and their proteomic profile. Human umbilical vein endothelial cells (HUVEC) and human pericytes from placenta (hPC-PL) were mixed in a fibrin suspension. The mild effect of sound-induced hydrodynamic forces condensed cells into architected geometries showing good fidelity to the numerical simulation of the physical process. Local cell density increased significantly within the patterned areas and the capillary-like structures formed following the cell density gradient. Over five days, these patterns were well-maintained, resulting in concentric circles and honeycomb-like structures.Proteomic analysis of the pre-condensed cells cultured for 5 days, revealed over 900 differentially expressed proteins when cells were preassembled through mild-hydrodynamic forces. Gene ontology (GO) enrichment analysis identified cellular components, molecular functions, and biological processes that were up- and down-regulated, providing insights regarding molecular processes influenced by the local density enhancement. Furthermore, we employed Ingenuity Pathway Analysis (IPA) to identify altered pathways and predict upstream regulators. Notably, VEGF-A emerged as one of the most prominent upstream regulators.Accordingly, this study initiates the unraveling of the changes in microcapillary networks at both molecular and proteins level induced by cell condensation obtained through sound patterning. The findings provide valuable insights for further investigation into sound patterning as a biofabrication technique for creating more complex microcapillary networks and advancing in vitro models. |
| first_indexed | 2024-04-24T14:28:31Z |
| format | Article |
| id | doaj.art-fbb680a5301a447ebc7342992fe203cd |
| institution | Directory Open Access Journal |
| issn | 2666-5344 |
| language | English |
| last_indexed | 2024-04-24T14:28:31Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomaterials and Biosystems |
| spelling | doaj.art-fbb680a5301a447ebc7342992fe203cd2024-04-03T04:27:30ZengElsevierBiomaterials and Biosystems2666-53442024-06-0114100094Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancementN. Di Marzio0R. Tognato1E. Della Bella2V. De Giorgis3M. Manfredi4A. Cochis5M. Alini6T. Serra7AO Research Institute Davos, 7270 Davos, Switzerland; Department of Health Sciences, Center for Translational Research and Autoimmune and Allergic Diseases (CAAD), University of Piemonte Orientale, 28100 Novara, ItalyAO Research Institute Davos, 7270 Davos, SwitzerlandAO Research Institute Davos, 7270 Davos, SwitzerlandDepartment of Translational Medicine, Center for Translational Research and Autoimmune and Allergic Diseases (CAAD), University of Piemonte Orientale, 28100 Novara, ItalyDepartment of Translational Medicine, Center for Translational Research and Autoimmune and Allergic Diseases (CAAD), University of Piemonte Orientale, 28100 Novara, ItalyDepartment of Health Sciences, Center for Translational Research and Autoimmune and Allergic Diseases (CAAD), University of Piemonte Orientale, 28100 Novara, ItalyAO Research Institute Davos, 7270 Davos, SwitzerlandAO Research Institute Davos, 7270 Davos, Switzerland; CTR Department, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, the Netherlands; Collaborative Research Partner, AO CMF CPP Bone Regeneration, Davos, Switzerland; Corresponding author at: AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos Platz, Switzerland.Spatial cell organization and biofabrication of microcapillary networks in vitro has a great potential in tissue engineering and regenerative medicine. This study explores the impact of local cell density enhancement achieved through an innovative sound-based patterning on microcapillary networks formation and their proteomic profile. Human umbilical vein endothelial cells (HUVEC) and human pericytes from placenta (hPC-PL) were mixed in a fibrin suspension. The mild effect of sound-induced hydrodynamic forces condensed cells into architected geometries showing good fidelity to the numerical simulation of the physical process. Local cell density increased significantly within the patterned areas and the capillary-like structures formed following the cell density gradient. Over five days, these patterns were well-maintained, resulting in concentric circles and honeycomb-like structures.Proteomic analysis of the pre-condensed cells cultured for 5 days, revealed over 900 differentially expressed proteins when cells were preassembled through mild-hydrodynamic forces. Gene ontology (GO) enrichment analysis identified cellular components, molecular functions, and biological processes that were up- and down-regulated, providing insights regarding molecular processes influenced by the local density enhancement. Furthermore, we employed Ingenuity Pathway Analysis (IPA) to identify altered pathways and predict upstream regulators. Notably, VEGF-A emerged as one of the most prominent upstream regulators.Accordingly, this study initiates the unraveling of the changes in microcapillary networks at both molecular and proteins level induced by cell condensation obtained through sound patterning. The findings provide valuable insights for further investigation into sound patterning as a biofabrication technique for creating more complex microcapillary networks and advancing in vitro models.http://www.sciencedirect.com/science/article/pii/S2666534424000072Bio-assemblySound patterningMicrocapillary networksCell density enhancementProteomic analysis |
| spellingShingle | N. Di Marzio R. Tognato E. Della Bella V. De Giorgis M. Manfredi A. Cochis M. Alini T. Serra Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement Biomaterials and Biosystems Bio-assembly Sound patterning Microcapillary networks Cell density enhancement Proteomic analysis |
| title | Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement |
| title_full | Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement |
| title_fullStr | Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement |
| title_full_unstemmed | Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement |
| title_short | Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement |
| title_sort | differential proteomics profile of microcapillary networks in response to sound pattern driven local cell density enhancement |
| topic | Bio-assembly Sound patterning Microcapillary networks Cell density enhancement Proteomic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2666534424000072 |
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