Visualizing the Spatial Distribution of <i>Arctium lappa</i> L. Root Components by MALDI-TOF Mass Spectrometry Imaging
This study is aimed at developing novel analytical methods to accurately visualize the spatial distribution of various endogenous components in <i>Arctium lappa</i> L. (<i>A. lappa</i>) roots, and to precisely guide the setting of pre-treatment operations during processing te...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/24/3957 |
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| author | Lingyu Li Zhichang Qiu Mingdi Jiang Bin Zhang Qiang Chen Chaojie Zhang Zhenjia Zheng Xuguang Qiao |
| author_facet | Lingyu Li Zhichang Qiu Mingdi Jiang Bin Zhang Qiang Chen Chaojie Zhang Zhenjia Zheng Xuguang Qiao |
| author_sort | Lingyu Li |
| collection | DOAJ |
| description | This study is aimed at developing novel analytical methods to accurately visualize the spatial distribution of various endogenous components in <i>Arctium lappa</i> L. (<i>A. lappa</i>) roots, and to precisely guide the setting of pre-treatment operations during processing technologies and understand plant metabolism process. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) imaging technology was used for visual demonstration of the in situ spatial distribution in <i>A. lappa</i> roots. This work consisted of four steps: matrix selection, section preparation, matrix coating, and MALDI-TOF MS imaging analysis. Consequently, eight saccharides, four caffeoylquinic acids, four flavonoids, six amino acids, one choline, and one phospholipid were imaged and four unidentified components were found. Saccharides were distributed in the center, whereas caffeoylquinic acids and flavonoids were mainly present in the epidermis and cortex. Furthermore, amino acids were mainly detected in the phloem, and choline in the cambium, while phosphatidylserine was found in the secondary phloem and cambium. This study demonstrated that MALDI-TOF MS imaging technology could provide a technical support to understand the spatial distribution of components in <i>A. lappa</i> roots, which would promote the processing technologies for <i>A. lappa</i> roots and help us to understand the plant metabolism process. |
| first_indexed | 2024-03-09T16:43:35Z |
| format | Article |
| id | doaj.art-fbb763761db64efaa882f48f49917121 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T16:43:35Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-fbb763761db64efaa882f48f499171212023-11-24T14:49:54ZengMDPI AGFoods2304-81582022-12-011124395710.3390/foods11243957Visualizing the Spatial Distribution of <i>Arctium lappa</i> L. Root Components by MALDI-TOF Mass Spectrometry ImagingLingyu Li0Zhichang Qiu1Mingdi Jiang2Bin Zhang3Qiang Chen4Chaojie Zhang5Zhenjia Zheng6Xuguang Qiao7Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai’an 271018, ChinaKey Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai’an 271018, ChinaDepartment of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Boston, MA 01003, USAKey Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai’an 271018, ChinaShandong Burdock Medical Research Institute Co., Ltd., 3230 Chongde 8th Avenue, Dezhou 253084, ChinaShandong Burdock Medical Research Institute Co., Ltd., 3230 Chongde 8th Avenue, Dezhou 253084, ChinaKey Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai’an 271018, ChinaKey Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai’an 271018, ChinaThis study is aimed at developing novel analytical methods to accurately visualize the spatial distribution of various endogenous components in <i>Arctium lappa</i> L. (<i>A. lappa</i>) roots, and to precisely guide the setting of pre-treatment operations during processing technologies and understand plant metabolism process. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) imaging technology was used for visual demonstration of the in situ spatial distribution in <i>A. lappa</i> roots. This work consisted of four steps: matrix selection, section preparation, matrix coating, and MALDI-TOF MS imaging analysis. Consequently, eight saccharides, four caffeoylquinic acids, four flavonoids, six amino acids, one choline, and one phospholipid were imaged and four unidentified components were found. Saccharides were distributed in the center, whereas caffeoylquinic acids and flavonoids were mainly present in the epidermis and cortex. Furthermore, amino acids were mainly detected in the phloem, and choline in the cambium, while phosphatidylserine was found in the secondary phloem and cambium. This study demonstrated that MALDI-TOF MS imaging technology could provide a technical support to understand the spatial distribution of components in <i>A. lappa</i> roots, which would promote the processing technologies for <i>A. lappa</i> roots and help us to understand the plant metabolism process.https://www.mdpi.com/2304-8158/11/24/3957<i>Arctium lappa</i> L.MALDI-TOF mass spectrometry imagingchemical componentsspatial distribution |
| spellingShingle | Lingyu Li Zhichang Qiu Mingdi Jiang Bin Zhang Qiang Chen Chaojie Zhang Zhenjia Zheng Xuguang Qiao Visualizing the Spatial Distribution of <i>Arctium lappa</i> L. Root Components by MALDI-TOF Mass Spectrometry Imaging Foods <i>Arctium lappa</i> L. MALDI-TOF mass spectrometry imaging chemical components spatial distribution |
| title | Visualizing the Spatial Distribution of <i>Arctium lappa</i> L. Root Components by MALDI-TOF Mass Spectrometry Imaging |
| title_full | Visualizing the Spatial Distribution of <i>Arctium lappa</i> L. Root Components by MALDI-TOF Mass Spectrometry Imaging |
| title_fullStr | Visualizing the Spatial Distribution of <i>Arctium lappa</i> L. Root Components by MALDI-TOF Mass Spectrometry Imaging |
| title_full_unstemmed | Visualizing the Spatial Distribution of <i>Arctium lappa</i> L. Root Components by MALDI-TOF Mass Spectrometry Imaging |
| title_short | Visualizing the Spatial Distribution of <i>Arctium lappa</i> L. Root Components by MALDI-TOF Mass Spectrometry Imaging |
| title_sort | visualizing the spatial distribution of i arctium lappa i l root components by maldi tof mass spectrometry imaging |
| topic | <i>Arctium lappa</i> L. MALDI-TOF mass spectrometry imaging chemical components spatial distribution |
| url | https://www.mdpi.com/2304-8158/11/24/3957 |
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