Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis
This study was to investigate the structure and rat fecal microbial fermentation properties of a polysaccharide fraction (PHP2) isolated from the red marine alga Porphyra haitanensis. PHP2 was characterized as a sulfated glucogalactan, with a hypothetical backbone structure of →4)Gα(1→6)G4Sβ(1→4)Glc...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001744 |
| _version_ | 1797726265576259584 |
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| author | Peilin Chen Lu Liu Zirun Cheng Yi Zhang Baodong Zheng Xiaoke Hu Hongliang Zeng |
| author_facet | Peilin Chen Lu Liu Zirun Cheng Yi Zhang Baodong Zheng Xiaoke Hu Hongliang Zeng |
| author_sort | Peilin Chen |
| collection | DOAJ |
| description | This study was to investigate the structure and rat fecal microbial fermentation properties of a polysaccharide fraction (PHP2) isolated from the red marine alga Porphyra haitanensis. PHP2 was characterized as a sulfated glucogalactan, with a hypothetical backbone structure of →4)Gα(1→6)G4Sβ(1→4)Glc(1→ and a side chain of Man(1→6)Glc. PHP2 had an irregular spherical chain conformation. The 16S rRNA sequence analysis revealed that PHP2 modulated the rat fecal micro-flora composition, with a similar effect to inulin, changing the dominant genus (Lactobacillus and Escherichia-Shigella) and promoting the growth of organisms that degrade sulfur-containing polysaccharides, such as Desulfovibrio, Ruminococcaceae_UCG-005, and Ruminococcus_2. PHP2 can promote production of acetic, propionic and butyric acid by rat fecal micro-flora. Prediction of metabolic function suggested that PHP2 could modulate cholesterol metabolism. The sulfated glucogalactan fermentation behavior may be associated with its monosaccharide composition, chain branching and chain conformation. PHP2 appeared to have considerable potential as functional food, and was associated with sulfur-containing polysaccharides in general. |
| first_indexed | 2024-03-12T10:43:12Z |
| format | Article |
| id | doaj.art-fbb7a8fede634182916a10d7d8477a82 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T10:43:12Z |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-fbb7a8fede634182916a10d7d8477a822023-09-02T08:02:15ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-01122596606Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensisPeilin Chen0Lu Liu1Zirun Cheng2Yi Zhang3Baodong Zheng4Xiaoke Hu5Hongliang Zeng6Engineering Research Center of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaEngineering Research Center of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaEngineering Research Center of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Corresponding authors.Engineering Research Center of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Corresponding authors.This study was to investigate the structure and rat fecal microbial fermentation properties of a polysaccharide fraction (PHP2) isolated from the red marine alga Porphyra haitanensis. PHP2 was characterized as a sulfated glucogalactan, with a hypothetical backbone structure of →4)Gα(1→6)G4Sβ(1→4)Glc(1→ and a side chain of Man(1→6)Glc. PHP2 had an irregular spherical chain conformation. The 16S rRNA sequence analysis revealed that PHP2 modulated the rat fecal micro-flora composition, with a similar effect to inulin, changing the dominant genus (Lactobacillus and Escherichia-Shigella) and promoting the growth of organisms that degrade sulfur-containing polysaccharides, such as Desulfovibrio, Ruminococcaceae_UCG-005, and Ruminococcus_2. PHP2 can promote production of acetic, propionic and butyric acid by rat fecal micro-flora. Prediction of metabolic function suggested that PHP2 could modulate cholesterol metabolism. The sulfated glucogalactan fermentation behavior may be associated with its monosaccharide composition, chain branching and chain conformation. PHP2 appeared to have considerable potential as functional food, and was associated with sulfur-containing polysaccharides in general.http://www.sciencedirect.com/science/article/pii/S2213453022001744Porphyra haitanensisStructure characterizationFecal microfloraShort-chain fatty acidsMetabolic function predictionPolysaccharide |
| spellingShingle | Peilin Chen Lu Liu Zirun Cheng Yi Zhang Baodong Zheng Xiaoke Hu Hongliang Zeng Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis Food Science and Human Wellness Porphyra haitanensis Structure characterization Fecal microflora Short-chain fatty acids Metabolic function prediction Polysaccharide |
| title | Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis |
| title_full | Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis |
| title_fullStr | Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis |
| title_full_unstemmed | Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis |
| title_short | Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis |
| title_sort | structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from porphyra haitanensis |
| topic | Porphyra haitanensis Structure characterization Fecal microflora Short-chain fatty acids Metabolic function prediction Polysaccharide |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001744 |
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