Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

The presence of the cap structure on the 5′-end of in vitro-transcribed (IVT) mRNA determines its translation and stability, underpinning its use in therapeutics. Both enzymatic and co-transcriptional capping may lead to incomplete positioning of the cap on newly synthesized RNA molecules. IVT mRNAs...

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Bibliographic Details
Main Authors: Irena Vlatkovic, János Ludwig, Gábor Boros, Gábor Tamás Szabó, Julia Reichert, Maximilian Buff, Markus Baiersdörfer, Jonas Reinholz, Azita Josefine Mahiny, Uğur Şahin, Katalin Karikó
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Pharmaceutics
Subjects:
mRNA capping efficiency
ribozyme
in vitro-transcribed (IVT) mRNA
cap
quality control
Online Access:https://www.mdpi.com/1999-4923/14/2/328

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