Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression
Summary: Cytoskeletal cross talk between actin filaments and microtubules is a common mechanism governing the assembly of cellular structures, i.e., during filopodia formation or cilia organization. However, potential actin-microtubule interactions during mammalian cell divisions are less well under...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-05-01
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| Series: | iScience |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004219301191 |
| _version_ | 1818284539624030208 |
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| author | Matthias Plessner Julian Knerr Robert Grosse |
| author_facet | Matthias Plessner Julian Knerr Robert Grosse |
| author_sort | Matthias Plessner |
| collection | DOAJ |
| description | Summary: Cytoskeletal cross talk between actin filaments and microtubules is a common mechanism governing the assembly of cellular structures, i.e., during filopodia formation or cilia organization. However, potential actin-microtubule interactions during mammalian cell divisions are less well understood. At mitotic entry, centrosomes propagate the formation of the mitotic spindle, thereby aligning individual chromosomes to the metaphase plate, a process coined chromosome congression. Here, we identify actin filament assembly spatially defined at centrosomes contemporaneously with spindle microtubules forming during prometaphase. We show that pharmacological Arp2/3 complex inhibition as well as overexpression of the Arp2/3 complex inhibitory protein Arpin decreased spindle actin. As a consequence, mitotic spindle formation is impaired, which resulted in disorganized chromosome congression and ultimately mitotic defects in non-transformed cells. Thus centrosomal Arp2/3 complex activity plays a role in the maintenance of genomic integrity during mitosis. : Biological Sciences; Cell Biology; Organizational Aspects of Cell Biology; Functional Aspects of Cell Biology Subject Areas: Biological Sciences, Cell Biology, Organizational Aspects of Cell Biology, Functional Aspects of Cell Biology |
| first_indexed | 2024-12-13T00:54:25Z |
| format | Article |
| id | doaj.art-fbb7feb182354737a2bef85b654cb2d2 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-13T00:54:25Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-fbb7feb182354737a2bef85b654cb2d22022-12-22T00:04:50ZengElsevieriScience2589-00422019-05-0115274281Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome CongressionMatthias Plessner0Julian Knerr1Robert Grosse2Institute of Pharmacology, University of Freiburg, Albertstr. 25, 79104 Freiburg, Germany; CIBSS - Centre for Integrative Biological Signalling Studies, University of Freiburg, Schänzlestr. 18, 79104 Freiburg, Germany; Corresponding authorInstitute of Pharmacology, University of Freiburg, Albertstr. 25, 79104 Freiburg, GermanyInstitute of Pharmacology, University of Freiburg, Albertstr. 25, 79104 Freiburg, Germany; CIBSS - Centre for Integrative Biological Signalling Studies, University of Freiburg, Schänzlestr. 18, 79104 Freiburg, Germany; Corresponding authorSummary: Cytoskeletal cross talk between actin filaments and microtubules is a common mechanism governing the assembly of cellular structures, i.e., during filopodia formation or cilia organization. However, potential actin-microtubule interactions during mammalian cell divisions are less well understood. At mitotic entry, centrosomes propagate the formation of the mitotic spindle, thereby aligning individual chromosomes to the metaphase plate, a process coined chromosome congression. Here, we identify actin filament assembly spatially defined at centrosomes contemporaneously with spindle microtubules forming during prometaphase. We show that pharmacological Arp2/3 complex inhibition as well as overexpression of the Arp2/3 complex inhibitory protein Arpin decreased spindle actin. As a consequence, mitotic spindle formation is impaired, which resulted in disorganized chromosome congression and ultimately mitotic defects in non-transformed cells. Thus centrosomal Arp2/3 complex activity plays a role in the maintenance of genomic integrity during mitosis. : Biological Sciences; Cell Biology; Organizational Aspects of Cell Biology; Functional Aspects of Cell Biology Subject Areas: Biological Sciences, Cell Biology, Organizational Aspects of Cell Biology, Functional Aspects of Cell Biologyhttp://www.sciencedirect.com/science/article/pii/S2589004219301191 |
| spellingShingle | Matthias Plessner Julian Knerr Robert Grosse Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression iScience |
| title | Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression |
| title_full | Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression |
| title_fullStr | Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression |
| title_full_unstemmed | Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression |
| title_short | Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression |
| title_sort | centrosomal actin assembly is required for proper mitotic spindle formation and chromosome congression |
| url | http://www.sciencedirect.com/science/article/pii/S2589004219301191 |
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