LncRNA4667 is dispensable for spermatogenesis and fertility in mice
Objective: Spermatogenesis is a complex process which is of vital importance for sexual reproduction. In many studies of spermatogenesis, the mRNAs, protein-coding genes, as well as small noncoding RNAs (ncRNAs) have been well characterized. However, there remain numerous questions despite previousl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health/LWW
2019-01-01
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| Series: | Reproductive and Developmental Medicine |
| Subjects: | |
| Online Access: | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2019;volume=3;issue=1;spage=18;epage=23;aulast=Dai |
| _version_ | 1818471053084590080 |
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| author | Yu-Bing Dai Yu Lin Ning Song Fei Sun |
| author_facet | Yu-Bing Dai Yu Lin Ning Song Fei Sun |
| author_sort | Yu-Bing Dai |
| collection | DOAJ |
| description | Objective: Spermatogenesis is a complex process which is of vital importance for sexual reproduction. In many studies of spermatogenesis, the mRNAs, protein-coding genes, as well as small noncoding RNAs (ncRNAs) have been well characterized. However, there remain numerous questions despite previously characterized molecular mechanisms. Long ncRNAs (lncRNAs) are a relatively new addition to our knowledge of ncRNAs. Limited studies have examined the function of lncRNAs in spermatogenesis. Therefore, we selected a testis-specific lncRNA, lncRNA4667, to analyze its potential role in spermatogenesis and male fertility.
Methods: In situ hybridization and quantitative reverse transcription polymerase chain reaction analyses were used to confirm testis-specific expression of lncRNA4667. LncRNA4667 knockout mice were generated using CRISPR/Cas9 technology. Histology, sperm counts, sperm motility, body parameters, and fertility were compared between wild-type and knockout mice (n = 8/group).
Results: Expression analysis showed that lncRNA4667 was testis specific and localized to round spermatids in seminiferous tubules of adult mouse testes. Mice homozygous for a null mutation of lncRNA4667 displayed normal spermatogenesis and fertility compared with wild-type mice.
Conclusions: These data indicate that lncRNA4667 is dispensable for spermatogenesis and fertility in mice, and the localization of lncRNA4667 makes it a useful marker for the identification of round spermatids in mice. |
| first_indexed | 2024-04-13T21:45:53Z |
| format | Article |
| id | doaj.art-fbb866dc132a49dc96a3c3ba3de977b9 |
| institution | Directory Open Access Journal |
| issn | 2096-2924 |
| language | English |
| last_indexed | 2024-04-13T21:45:53Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Health/LWW |
| record_format | Article |
| series | Reproductive and Developmental Medicine |
| spelling | doaj.art-fbb866dc132a49dc96a3c3ba3de977b92022-12-22T02:28:35ZengWolters Kluwer Health/LWWReproductive and Developmental Medicine2096-29242019-01-0131182310.4103/2096-2924.255985LncRNA4667 is dispensable for spermatogenesis and fertility in miceYu-Bing DaiYu LinNing SongFei SunObjective: Spermatogenesis is a complex process which is of vital importance for sexual reproduction. In many studies of spermatogenesis, the mRNAs, protein-coding genes, as well as small noncoding RNAs (ncRNAs) have been well characterized. However, there remain numerous questions despite previously characterized molecular mechanisms. Long ncRNAs (lncRNAs) are a relatively new addition to our knowledge of ncRNAs. Limited studies have examined the function of lncRNAs in spermatogenesis. Therefore, we selected a testis-specific lncRNA, lncRNA4667, to analyze its potential role in spermatogenesis and male fertility. Methods: In situ hybridization and quantitative reverse transcription polymerase chain reaction analyses were used to confirm testis-specific expression of lncRNA4667. LncRNA4667 knockout mice were generated using CRISPR/Cas9 technology. Histology, sperm counts, sperm motility, body parameters, and fertility were compared between wild-type and knockout mice (n = 8/group). Results: Expression analysis showed that lncRNA4667 was testis specific and localized to round spermatids in seminiferous tubules of adult mouse testes. Mice homozygous for a null mutation of lncRNA4667 displayed normal spermatogenesis and fertility compared with wild-type mice. Conclusions: These data indicate that lncRNA4667 is dispensable for spermatogenesis and fertility in mice, and the localization of lncRNA4667 makes it a useful marker for the identification of round spermatids in mice.http://www.repdevmed.org/article.asp?issn=2096-2924;year=2019;volume=3;issue=1;spage=18;epage=23;aulast=DaiLong Noncoding RNA; Male Infertility; Spermatogenesis |
| spellingShingle | Yu-Bing Dai Yu Lin Ning Song Fei Sun LncRNA4667 is dispensable for spermatogenesis and fertility in mice Reproductive and Developmental Medicine Long Noncoding RNA; Male Infertility; Spermatogenesis |
| title | LncRNA4667 is dispensable for spermatogenesis and fertility in mice |
| title_full | LncRNA4667 is dispensable for spermatogenesis and fertility in mice |
| title_fullStr | LncRNA4667 is dispensable for spermatogenesis and fertility in mice |
| title_full_unstemmed | LncRNA4667 is dispensable for spermatogenesis and fertility in mice |
| title_short | LncRNA4667 is dispensable for spermatogenesis and fertility in mice |
| title_sort | lncrna4667 is dispensable for spermatogenesis and fertility in mice |
| topic | Long Noncoding RNA; Male Infertility; Spermatogenesis |
| url | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2019;volume=3;issue=1;spage=18;epage=23;aulast=Dai |
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