Human NK cell subset functions are differentially affected by adipokines.
BACKGROUND: Obesity is a risk factor for various types of infectious diseases and cancer. The increase in adipose tissue causes alterations in both adipogenesis and the production of adipocyte-secreted proteins (adipokines). Since natural killer (NK) cells are the host's primary defense against...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3787100?pdf=render |
| _version_ | 1817997302725345280 |
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| author | Lena Huebner Stefan Engeli Christiane D Wrann Lilia Goudeva Tobias Laue Heike Kielstein |
| author_facet | Lena Huebner Stefan Engeli Christiane D Wrann Lilia Goudeva Tobias Laue Heike Kielstein |
| author_sort | Lena Huebner |
| collection | DOAJ |
| description | BACKGROUND: Obesity is a risk factor for various types of infectious diseases and cancer. The increase in adipose tissue causes alterations in both adipogenesis and the production of adipocyte-secreted proteins (adipokines). Since natural killer (NK) cells are the host's primary defense against virus-infected and tumor cells, we investigated how adipocyte-conditioned medium (ACM) affects functions of two distinct human NK cell subsets. METHODS: Isolated human peripheral blood mononuclear cells (PBMCs) were cultured with various concentrations of human and murine ACM harvested on two different days during adipogenesis and analyzed by fluorescent-activated cell sorting (FACS). RESULTS: FACS analyses showed that the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), granzyme A (GzmA) and interferon (IFN)-γ in NK cells was regulated in a subset-specific manner. ACM treatment altered IFN-γ expression in CD56(dim) NK cells. The production of GzmA in CD56(bright) NK cells was differentially affected by the distinct adipokine compositions harvested at different states of adipogenesis. Comparison of the treatment with either human or murine ACM revealed that adipokine-induced effects on NK cell expression of the leptin receptor (Ob-R), TRAIL and IFN-γ were species-specific. CONCLUSION: Considering the growing prevalence of obesity and the various disorders related to it, the present study provides further insights into the roles human NK cell subsets play in the obesity-associated state of chronic low-grade inflammation. |
| first_indexed | 2024-04-14T02:35:07Z |
| format | Article |
| id | doaj.art-fbbc29a2fc8a4267bdc8eeba3683ffe7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T02:35:07Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fbbc29a2fc8a4267bdc8eeba3683ffe72022-12-22T02:17:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7570310.1371/journal.pone.0075703Human NK cell subset functions are differentially affected by adipokines.Lena HuebnerStefan EngeliChristiane D WrannLilia GoudevaTobias LaueHeike KielsteinBACKGROUND: Obesity is a risk factor for various types of infectious diseases and cancer. The increase in adipose tissue causes alterations in both adipogenesis and the production of adipocyte-secreted proteins (adipokines). Since natural killer (NK) cells are the host's primary defense against virus-infected and tumor cells, we investigated how adipocyte-conditioned medium (ACM) affects functions of two distinct human NK cell subsets. METHODS: Isolated human peripheral blood mononuclear cells (PBMCs) were cultured with various concentrations of human and murine ACM harvested on two different days during adipogenesis and analyzed by fluorescent-activated cell sorting (FACS). RESULTS: FACS analyses showed that the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), granzyme A (GzmA) and interferon (IFN)-γ in NK cells was regulated in a subset-specific manner. ACM treatment altered IFN-γ expression in CD56(dim) NK cells. The production of GzmA in CD56(bright) NK cells was differentially affected by the distinct adipokine compositions harvested at different states of adipogenesis. Comparison of the treatment with either human or murine ACM revealed that adipokine-induced effects on NK cell expression of the leptin receptor (Ob-R), TRAIL and IFN-γ were species-specific. CONCLUSION: Considering the growing prevalence of obesity and the various disorders related to it, the present study provides further insights into the roles human NK cell subsets play in the obesity-associated state of chronic low-grade inflammation.http://europepmc.org/articles/PMC3787100?pdf=render |
| spellingShingle | Lena Huebner Stefan Engeli Christiane D Wrann Lilia Goudeva Tobias Laue Heike Kielstein Human NK cell subset functions are differentially affected by adipokines. PLoS ONE |
| title | Human NK cell subset functions are differentially affected by adipokines. |
| title_full | Human NK cell subset functions are differentially affected by adipokines. |
| title_fullStr | Human NK cell subset functions are differentially affected by adipokines. |
| title_full_unstemmed | Human NK cell subset functions are differentially affected by adipokines. |
| title_short | Human NK cell subset functions are differentially affected by adipokines. |
| title_sort | human nk cell subset functions are differentially affected by adipokines |
| url | http://europepmc.org/articles/PMC3787100?pdf=render |
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