Experimental Approach Reveals the Role of alx1 in the Evolution of the Echinoderm Larval Skeleton.
Over the course of evolution, the acquisition of novel structures has ultimately led to wide variation in morphology among extant multicellular organisms. Thus, the origins of genetic systems for new morphological structures are a subject of great interest in evolutionary biology. The larval skeleto...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4750990?pdf=render |
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| author | Hiroyuki Koga Haruka Fujitani Yoshiaki Morino Norio Miyamoto Jun Tsuchimoto Tomoko F Shibata Masafumi Nozawa Shuji Shigenobu Atsushi Ogura Kazunori Tachibana Masato Kiyomoto Shonan Amemiya Hiroshi Wada |
| author_facet | Hiroyuki Koga Haruka Fujitani Yoshiaki Morino Norio Miyamoto Jun Tsuchimoto Tomoko F Shibata Masafumi Nozawa Shuji Shigenobu Atsushi Ogura Kazunori Tachibana Masato Kiyomoto Shonan Amemiya Hiroshi Wada |
| author_sort | Hiroyuki Koga |
| collection | DOAJ |
| description | Over the course of evolution, the acquisition of novel structures has ultimately led to wide variation in morphology among extant multicellular organisms. Thus, the origins of genetic systems for new morphological structures are a subject of great interest in evolutionary biology. The larval skeleton is a novel structure acquired in some echinoderm lineages via the activation of the adult skeletogenic machinery. Previously, VEGF signaling was suggested to have played an important role in the acquisition of the larval skeleton. In the present study, we compared expression patterns of Alx genes among echinoderm classes to further explore the factors involved in the acquisition of a larval skeleton. We found that the alx1 gene, originally described as crucial for sea urchin skeletogenesis, may have also played an essential role in the evolution of the larval skeleton. Unlike those echinoderms that have a larval skeleton, we found that alx1 of starfish was barely expressed in early larvae that have no skeleton. When alx1 overexpression was induced via injection of alx1 mRNA into starfish eggs, the expression patterns of certain genes, including those possibly involved in skeletogenesis, were altered. This suggested that a portion of the skeletogenic program was induced solely by alx1. However, we observed no obvious external phenotype or skeleton. We concluded that alx1 was necessary but not sufficient for the acquisition of the larval skeleton, which, in fact, requires several genetic events. Based on these results, we discuss how the larval expression of alx1 contributed to the acquisition of the larval skeleton in the putative ancestral lineage of echinoderms. |
| first_indexed | 2024-12-10T13:18:02Z |
| format | Article |
| id | doaj.art-fbc397cbe37849e692e427dd87dffe4c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T13:18:02Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fbc397cbe37849e692e427dd87dffe4c2022-12-22T01:47:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01112e014906710.1371/journal.pone.0149067Experimental Approach Reveals the Role of alx1 in the Evolution of the Echinoderm Larval Skeleton.Hiroyuki KogaHaruka FujitaniYoshiaki MorinoNorio MiyamotoJun TsuchimotoTomoko F ShibataMasafumi NozawaShuji ShigenobuAtsushi OguraKazunori TachibanaMasato KiyomotoShonan AmemiyaHiroshi WadaOver the course of evolution, the acquisition of novel structures has ultimately led to wide variation in morphology among extant multicellular organisms. Thus, the origins of genetic systems for new morphological structures are a subject of great interest in evolutionary biology. The larval skeleton is a novel structure acquired in some echinoderm lineages via the activation of the adult skeletogenic machinery. Previously, VEGF signaling was suggested to have played an important role in the acquisition of the larval skeleton. In the present study, we compared expression patterns of Alx genes among echinoderm classes to further explore the factors involved in the acquisition of a larval skeleton. We found that the alx1 gene, originally described as crucial for sea urchin skeletogenesis, may have also played an essential role in the evolution of the larval skeleton. Unlike those echinoderms that have a larval skeleton, we found that alx1 of starfish was barely expressed in early larvae that have no skeleton. When alx1 overexpression was induced via injection of alx1 mRNA into starfish eggs, the expression patterns of certain genes, including those possibly involved in skeletogenesis, were altered. This suggested that a portion of the skeletogenic program was induced solely by alx1. However, we observed no obvious external phenotype or skeleton. We concluded that alx1 was necessary but not sufficient for the acquisition of the larval skeleton, which, in fact, requires several genetic events. Based on these results, we discuss how the larval expression of alx1 contributed to the acquisition of the larval skeleton in the putative ancestral lineage of echinoderms.http://europepmc.org/articles/PMC4750990?pdf=render |
| spellingShingle | Hiroyuki Koga Haruka Fujitani Yoshiaki Morino Norio Miyamoto Jun Tsuchimoto Tomoko F Shibata Masafumi Nozawa Shuji Shigenobu Atsushi Ogura Kazunori Tachibana Masato Kiyomoto Shonan Amemiya Hiroshi Wada Experimental Approach Reveals the Role of alx1 in the Evolution of the Echinoderm Larval Skeleton. PLoS ONE |
| title | Experimental Approach Reveals the Role of alx1 in the Evolution of the Echinoderm Larval Skeleton. |
| title_full | Experimental Approach Reveals the Role of alx1 in the Evolution of the Echinoderm Larval Skeleton. |
| title_fullStr | Experimental Approach Reveals the Role of alx1 in the Evolution of the Echinoderm Larval Skeleton. |
| title_full_unstemmed | Experimental Approach Reveals the Role of alx1 in the Evolution of the Echinoderm Larval Skeleton. |
| title_short | Experimental Approach Reveals the Role of alx1 in the Evolution of the Echinoderm Larval Skeleton. |
| title_sort | experimental approach reveals the role of alx1 in the evolution of the echinoderm larval skeleton |
| url | http://europepmc.org/articles/PMC4750990?pdf=render |
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