φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3
Abstract Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-01-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-021-01341-2 |
| _version_ | 1819229358747811840 |
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| author | Karolina Filik Bożena Szermer-Olearnik Joanna Niedziółka-Jönson Ewa Roźniecka Jarosław Ciekot Anna Pyra Irwin Matyjaszczyk Mikael Skurnik Ewa Brzozowska |
| author_facet | Karolina Filik Bożena Szermer-Olearnik Joanna Niedziółka-Jönson Ewa Roźniecka Jarosław Ciekot Anna Pyra Irwin Matyjaszczyk Mikael Skurnik Ewa Brzozowska |
| author_sort | Karolina Filik |
| collection | DOAJ |
| description | Abstract Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestion of contaminated food. Yersiniosis is considered a rare disease, but recent studies indicate that it is overlooked in the diagnostic process therefore the infections with this bacterium are not often identified. Reliable diagnosis of Yersiniosis by culturing is difficult due to the slow growth of the bacteria easily overgrown by other more rapidly growing microbes unless selec-tive growth media is used. Phage adhesins recognizing bacteria in a specific manner can be an excellent diagnostic tool, es-pecially in the diagnosis of pathogens difficult for culturing. In this study, it was shown that Gp17, the tail fiber protein (TFP) of phage φYeO3-12, specifically recognizes only the pathogenic Yersinia enterocolitica serotype O:3 (YeO:3) bacteria. The ELISA test used in this work confirmed the specific interaction of this protein with YeO:3 and demonstrated a promising tool for developing the pathogen recognition method based on phage adhesins. |
| first_indexed | 2024-12-23T11:11:55Z |
| format | Article |
| id | doaj.art-fbc3b7acf1a540539cbbacff6e903794 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-23T11:11:55Z |
| publishDate | 2022-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-fbc3b7acf1a540539cbbacff6e9037942022-12-21T17:49:20ZengSpringerOpenAMB Express2191-08552022-01-0112111010.1186/s13568-021-01341-2φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3Karolina Filik0Bożena Szermer-Olearnik1Joanna Niedziółka-Jönson2Ewa Roźniecka3Jarosław Ciekot4Anna Pyra5Irwin Matyjaszczyk6Mikael Skurnik7Ewa Brzozowska8Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesHirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesInstitute of Physical Chemistry, Polish Academy of SciencesInstitute of Physical Chemistry, Polish Academy of SciencesHirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesFaculty of Chemistry, University of WroclawDepartment of Mycology and Genetics, Institute of Genetics and Microbiology, University of WrocławDepartment of Bacteriology and Immunology, Faculty of Medicine, Human Microbiome Research Program, University of HelsinkiHirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesAbstract Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestion of contaminated food. Yersiniosis is considered a rare disease, but recent studies indicate that it is overlooked in the diagnostic process therefore the infections with this bacterium are not often identified. Reliable diagnosis of Yersiniosis by culturing is difficult due to the slow growth of the bacteria easily overgrown by other more rapidly growing microbes unless selec-tive growth media is used. Phage adhesins recognizing bacteria in a specific manner can be an excellent diagnostic tool, es-pecially in the diagnosis of pathogens difficult for culturing. In this study, it was shown that Gp17, the tail fiber protein (TFP) of phage φYeO3-12, specifically recognizes only the pathogenic Yersinia enterocolitica serotype O:3 (YeO:3) bacteria. The ELISA test used in this work confirmed the specific interaction of this protein with YeO:3 and demonstrated a promising tool for developing the pathogen recognition method based on phage adhesins.https://doi.org/10.1186/s13568-021-01341-2PhageYersiniosisTail fiber proteinPhage adhesinsYersinia enterocoliticaDiagnostic |
| spellingShingle | Karolina Filik Bożena Szermer-Olearnik Joanna Niedziółka-Jönson Ewa Roźniecka Jarosław Ciekot Anna Pyra Irwin Matyjaszczyk Mikael Skurnik Ewa Brzozowska φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3 AMB Express Phage Yersiniosis Tail fiber protein Phage adhesins Yersinia enterocolitica Diagnostic |
| title | φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3 |
| title_full | φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3 |
| title_fullStr | φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3 |
| title_full_unstemmed | φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3 |
| title_short | φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3 |
| title_sort | φyeo3 12 phage tail fiber gp17 as a promising high specific tool for recognition of yersinia enterocolitica pathogenic serotype o 3 |
| topic | Phage Yersiniosis Tail fiber protein Phage adhesins Yersinia enterocolitica Diagnostic |
| url | https://doi.org/10.1186/s13568-021-01341-2 |
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