Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
<b>: </b>African swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken...
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MDPI AG
2020-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/3/171 |
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| author | Estefanía Cadenas-Fernández Jose M. Sánchez-Vizcaíno Aleksandra Kosowska Belén Rivera Francisco Mayoral-Alegre Antonio Rodríguez-Bertos Jianxiu Yao Jocelyn Bray Shehnaz Lokhandwala Waithaka Mwangi Jose A. Barasona |
| author_facet | Estefanía Cadenas-Fernández Jose M. Sánchez-Vizcaíno Aleksandra Kosowska Belén Rivera Francisco Mayoral-Alegre Antonio Rodríguez-Bertos Jianxiu Yao Jocelyn Bray Shehnaz Lokhandwala Waithaka Mwangi Jose A. Barasona |
| author_sort | Estefanía Cadenas-Fernández |
| collection | DOAJ |
| description | <b>: </b>African swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insufficient and an effective vaccine against ASFV needs to be urgently developed. We hypothesized that immunization with a cocktail of thirty-five rationally selected antigens would improve the protective efficacy of subunit vaccine prototypes given that the combination of fewer immunogenic antigens (between 2 and 22) has failed to elicit protective efficacy. To this end, immunogenicity and efficacy of thirty-five adenovirus-vectored ASFV antigens were evaluated in wild boar. The treated animals were divided into different groups to test the use of BioMize adjuvant and different inoculation strategies. Forty-eight days after priming, the nine treated and two control wild boar were challenged with the virulent ASFV Arm07 isolate. All animals showed clinical signs and pathological findings consistent with ASF. This lack of protection is in line with other studies with subunit vaccine prototypes, demonstrating that there is still much room for improvement to obtain an effective subunit ASFV vaccine. |
| first_indexed | 2024-04-13T08:06:24Z |
| format | Article |
| id | doaj.art-fbc97c1aa46e4dfb8346abe53d9866e8 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-13T08:06:24Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-fbc97c1aa46e4dfb8346abe53d9866e82022-12-22T02:55:09ZengMDPI AGPathogens2076-08172020-02-019317110.3390/pathogens9030171pathogens9030171Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild BoarEstefanía Cadenas-Fernández0Jose M. Sánchez-Vizcaíno1Aleksandra Kosowska2Belén Rivera3Francisco Mayoral-Alegre4Antonio Rodríguez-Bertos5Jianxiu Yao6Jocelyn Bray7Shehnaz Lokhandwala8Waithaka Mwangi9Jose A. Barasona10VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USADepartment of Veterinary Pathobiology, Texas A&M University, College Station Texas, TX 77843-4467, USADepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USADepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USAVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain<b>: </b>African swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insufficient and an effective vaccine against ASFV needs to be urgently developed. We hypothesized that immunization with a cocktail of thirty-five rationally selected antigens would improve the protective efficacy of subunit vaccine prototypes given that the combination of fewer immunogenic antigens (between 2 and 22) has failed to elicit protective efficacy. To this end, immunogenicity and efficacy of thirty-five adenovirus-vectored ASFV antigens were evaluated in wild boar. The treated animals were divided into different groups to test the use of BioMize adjuvant and different inoculation strategies. Forty-eight days after priming, the nine treated and two control wild boar were challenged with the virulent ASFV Arm07 isolate. All animals showed clinical signs and pathological findings consistent with ASF. This lack of protection is in line with other studies with subunit vaccine prototypes, demonstrating that there is still much room for improvement to obtain an effective subunit ASFV vaccine.https://www.mdpi.com/2076-0817/9/3/171african swine feveradenovirusarmenia07/arm07eurasian wild boarimmune responsesubunit vaccineprotective efficacy |
| spellingShingle | Estefanía Cadenas-Fernández Jose M. Sánchez-Vizcaíno Aleksandra Kosowska Belén Rivera Francisco Mayoral-Alegre Antonio Rodríguez-Bertos Jianxiu Yao Jocelyn Bray Shehnaz Lokhandwala Waithaka Mwangi Jose A. Barasona Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar Pathogens african swine fever adenovirus armenia07/arm07 eurasian wild boar immune response subunit vaccine protective efficacy |
| title | Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar |
| title_full | Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar |
| title_fullStr | Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar |
| title_full_unstemmed | Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar |
| title_short | Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar |
| title_sort | adenovirus vectored african swine fever virus antigens cocktail is not protective against virulent arm07 isolate in eurasian wild boar |
| topic | african swine fever adenovirus armenia07/arm07 eurasian wild boar immune response subunit vaccine protective efficacy |
| url | https://www.mdpi.com/2076-0817/9/3/171 |
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