Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection
Tumor-associated cell-free DNA (cfDNA) is a dynamic biomarker for genetic analysis, early diagnosis and clinical treatment of cancers. However, its detection has limitations because of its low abundance in blood or other complex bodily fluids. Herein, we developed an ultrasensitive cfDNA electrochem...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/4/666 |
| _version_ | 1827653476343087104 |
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| author | Chenguang Wang Wei Wang Yi Xu Xiaoshuang Zhao Shuainan Li Qiuling Qian Xianqiang Mi |
| author_facet | Chenguang Wang Wei Wang Yi Xu Xiaoshuang Zhao Shuainan Li Qiuling Qian Xianqiang Mi |
| author_sort | Chenguang Wang |
| collection | DOAJ |
| description | Tumor-associated cell-free DNA (cfDNA) is a dynamic biomarker for genetic analysis, early diagnosis and clinical treatment of cancers. However, its detection has limitations because of its low abundance in blood or other complex bodily fluids. Herein, we developed an ultrasensitive cfDNA electrochemical biosensor (E-cfDNA sensor) based on tetrahedral DNA framework (TDF)-modified gold nanoparticles (Au NPs) with an interface for cfDNA detection. By accurately controlling the numbers of base pairs on each DNA framework, three types of TDFs were programmed: 26 base pairs of TDF; 17 base pairs of TDF; and 7 base pairs of TDF (TDF-26, TDF-16 and TDF-7, respectively). We also combined the TDF with hybridization chain reaction (HCR) to achieve signal amplification. Under optimal conditions, we detected the breast cancer susceptibility gene 1 (BRCA-1), a representative cfDNA closely related to breast cancer. An ultra-low detection limit of 1 aM with a linear range from 1 aM to 1 pM by TDF-26 was obtained, which was superior to the existing methods. Each type of TDF has excellent discrimination ability, which can distinguish single mismatch. More significantly, we also detected BRCA-1 in mimic serum samples, demonstrating that the E-cfDNA sensor has potential use in clinical research. |
| first_indexed | 2024-03-09T21:19:23Z |
| format | Article |
| id | doaj.art-fbcca7aa82d84ce18405b06011200639 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T21:19:23Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-fbcca7aa82d84ce18405b060112006392023-11-23T21:26:09ZengMDPI AGNanomaterials2079-49912022-02-0112466610.3390/nano12040666Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA DetectionChenguang Wang0Wei Wang1Yi Xu2Xiaoshuang Zhao3Shuainan Li4Qiuling Qian5Xianqiang Mi6Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaShanghai Pudong New District Zhoupu Hospital, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaState Key Laboratory of Functional Material for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaTumor-associated cell-free DNA (cfDNA) is a dynamic biomarker for genetic analysis, early diagnosis and clinical treatment of cancers. However, its detection has limitations because of its low abundance in blood or other complex bodily fluids. Herein, we developed an ultrasensitive cfDNA electrochemical biosensor (E-cfDNA sensor) based on tetrahedral DNA framework (TDF)-modified gold nanoparticles (Au NPs) with an interface for cfDNA detection. By accurately controlling the numbers of base pairs on each DNA framework, three types of TDFs were programmed: 26 base pairs of TDF; 17 base pairs of TDF; and 7 base pairs of TDF (TDF-26, TDF-16 and TDF-7, respectively). We also combined the TDF with hybridization chain reaction (HCR) to achieve signal amplification. Under optimal conditions, we detected the breast cancer susceptibility gene 1 (BRCA-1), a representative cfDNA closely related to breast cancer. An ultra-low detection limit of 1 aM with a linear range from 1 aM to 1 pM by TDF-26 was obtained, which was superior to the existing methods. Each type of TDF has excellent discrimination ability, which can distinguish single mismatch. More significantly, we also detected BRCA-1 in mimic serum samples, demonstrating that the E-cfDNA sensor has potential use in clinical research.https://www.mdpi.com/2079-4991/12/4/666tetrahedral DNA frameworkgold nanoparticleshybridization chain reactioncell-free DNAelectrochemical biosenors |
| spellingShingle | Chenguang Wang Wei Wang Yi Xu Xiaoshuang Zhao Shuainan Li Qiuling Qian Xianqiang Mi Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection Nanomaterials tetrahedral DNA framework gold nanoparticles hybridization chain reaction cell-free DNA electrochemical biosenors |
| title | Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection |
| title_full | Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection |
| title_fullStr | Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection |
| title_full_unstemmed | Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection |
| title_short | Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection |
| title_sort | tetrahedral dna framework programmed electrochemical biosenors with gold nanoparticles for ultrasensitive cell free dna detection |
| topic | tetrahedral DNA framework gold nanoparticles hybridization chain reaction cell-free DNA electrochemical biosenors |
| url | https://www.mdpi.com/2079-4991/12/4/666 |
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