A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis
Metaphase chromosome spreading is the most crucial step required for successful karyotyping and FISH analysis. These two techniques are routinely used in cytogenetics to assess the chromosome abnormalities. The spreading process has been studied for years but it is still considered an art more than...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-04-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-666X/5/2/158 |
| _version_ | 1818858207865470976 |
|---|---|
| author | Dorota Kwasny Olga Mednova Indumathi Vedarethinam Maria Dimaki Asli Silahtaroglu Zeynep Tümer Kristoffer Almdal Winnie E. Svendsen |
| author_facet | Dorota Kwasny Olga Mednova Indumathi Vedarethinam Maria Dimaki Asli Silahtaroglu Zeynep Tümer Kristoffer Almdal Winnie E. Svendsen |
| author_sort | Dorota Kwasny |
| collection | DOAJ |
| description | Metaphase chromosome spreading is the most crucial step required for successful karyotyping and FISH analysis. These two techniques are routinely used in cytogenetics to assess the chromosome abnormalities. The spreading process has been studied for years but it is still considered an art more than a science. The chromosome spreading greatly depends on the environmental conditions such as humidity and temperature, which govern the evaporation of fixative, in which the cells are suspended. The spreading is normally performed manually in ambient conditions on glass slides, which are hydrophilic, and thus allow for better quality spreads. Further cytogenetic analysis depends on the quality of the spreads, which is dependent on the skills of the personnel and is thus limited to laboratory settings. Here, we present a semi-closed microfluidic chip for preparation of the metaphase spreads on a glass and a Topasr substrate rendered more hydrophilic by oxygen plasma treatment coupled with photografting. The device consists of a microfluidic chamber with perfusion holes that facilitate the evaporation of fixative and reliable formation of the spreads. The usability of the chromosome spreads formed on the glass and the Topasr slide is tested by performing FISH analysis. |
| first_indexed | 2024-12-19T08:52:38Z |
| format | Article |
| id | doaj.art-fbcd5627515641de927066c97a39e6df |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-19T08:52:38Z |
| publishDate | 2014-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-fbcd5627515641de927066c97a39e6df2022-12-21T20:28:42ZengMDPI AGMicromachines2072-666X2014-04-015215817010.3390/mi5020158mi5020158A Semi-Closed Device for Chromosome Spreading for Cytogenetic AnalysisDorota Kwasny0Olga Mednova1Indumathi Vedarethinam2Maria Dimaki3Asli Silahtaroglu4Zeynep Tümer5Kristoffer Almdal6Winnie E. Svendsen7Department of Micro- and Nanotechnology, Technical University of Denmark, Orsteds Plads, 2800 Kgs. Lyngby 2800, DenmarkDepartment of Micro- and Nanotechnology, Technical University of Denmark, Orsteds Plads, 2800 Kgs. Lyngby 2800, DenmarkCenter for Molecular Biology 'Severo Ochoa', UAM campus Cantoblano, Madrid 28049, SpainDepartment of Micro- and Nanotechnology, Technical University of Denmark, Orsteds Plads, 2800 Kgs. Lyngby 2800, DenmarkFaculty of Health Sciences, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen 2200, DenmarkApplied Human Molecular Genetics, Kennedy Center, Copenhagen University Hospital, Rigshospitalet, Glostrup 2600, DenmarkDepartment of Micro- and Nanotechnology, Technical University of Denmark, Orsteds Plads, 2800 Kgs. Lyngby 2800, DenmarkDepartment of Micro- and Nanotechnology, Technical University of Denmark, Orsteds Plads, 2800 Kgs. Lyngby 2800, DenmarkMetaphase chromosome spreading is the most crucial step required for successful karyotyping and FISH analysis. These two techniques are routinely used in cytogenetics to assess the chromosome abnormalities. The spreading process has been studied for years but it is still considered an art more than a science. The chromosome spreading greatly depends on the environmental conditions such as humidity and temperature, which govern the evaporation of fixative, in which the cells are suspended. The spreading is normally performed manually in ambient conditions on glass slides, which are hydrophilic, and thus allow for better quality spreads. Further cytogenetic analysis depends on the quality of the spreads, which is dependent on the skills of the personnel and is thus limited to laboratory settings. Here, we present a semi-closed microfluidic chip for preparation of the metaphase spreads on a glass and a Topasr substrate rendered more hydrophilic by oxygen plasma treatment coupled with photografting. The device consists of a microfluidic chamber with perfusion holes that facilitate the evaporation of fixative and reliable formation of the spreads. The usability of the chromosome spreads formed on the glass and the Topasr slide is tested by performing FISH analysis.http://www.mdpi.com/2072-666X/5/2/158cytogenetic analysisevaporationchromosome spreadingmicrofluidic chip |
| spellingShingle | Dorota Kwasny Olga Mednova Indumathi Vedarethinam Maria Dimaki Asli Silahtaroglu Zeynep Tümer Kristoffer Almdal Winnie E. Svendsen A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis Micromachines cytogenetic analysis evaporation chromosome spreading microfluidic chip |
| title | A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis |
| title_full | A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis |
| title_fullStr | A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis |
| title_full_unstemmed | A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis |
| title_short | A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis |
| title_sort | semi closed device for chromosome spreading for cytogenetic analysis |
| topic | cytogenetic analysis evaporation chromosome spreading microfluidic chip |
| url | http://www.mdpi.com/2072-666X/5/2/158 |
| work_keys_str_mv | AT dorotakwasny asemicloseddeviceforchromosomespreadingforcytogeneticanalysis AT olgamednova asemicloseddeviceforchromosomespreadingforcytogeneticanalysis AT indumathivedarethinam asemicloseddeviceforchromosomespreadingforcytogeneticanalysis AT mariadimaki asemicloseddeviceforchromosomespreadingforcytogeneticanalysis AT aslisilahtaroglu asemicloseddeviceforchromosomespreadingforcytogeneticanalysis AT zeyneptumer asemicloseddeviceforchromosomespreadingforcytogeneticanalysis AT kristofferalmdal asemicloseddeviceforchromosomespreadingforcytogeneticanalysis AT winnieesvendsen asemicloseddeviceforchromosomespreadingforcytogeneticanalysis AT dorotakwasny semicloseddeviceforchromosomespreadingforcytogeneticanalysis AT olgamednova semicloseddeviceforchromosomespreadingforcytogeneticanalysis AT indumathivedarethinam semicloseddeviceforchromosomespreadingforcytogeneticanalysis AT mariadimaki semicloseddeviceforchromosomespreadingforcytogeneticanalysis AT aslisilahtaroglu semicloseddeviceforchromosomespreadingforcytogeneticanalysis AT zeyneptumer semicloseddeviceforchromosomespreadingforcytogeneticanalysis AT kristofferalmdal semicloseddeviceforchromosomespreadingforcytogeneticanalysis AT winnieesvendsen semicloseddeviceforchromosomespreadingforcytogeneticanalysis |