Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro
Background: Chlorothalonil and acetamiprid are chemical pesticides commonly used in agricultural production and have been shown to have negative effects on bee’s fitness. Despite many studies have revealed that honey bee (Apis mellifera L.) larvae are posting a high risk on exposure to pesticides, b...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1114403/full |
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| author | Ying Lu Ying Lu Jing Gao Tong Wu Bo Han Bingnan Qian Min Shi Sa Yang Qingyun Diao Chunya Bu Pingli Dai |
| author_facet | Ying Lu Ying Lu Jing Gao Tong Wu Bo Han Bingnan Qian Min Shi Sa Yang Qingyun Diao Chunya Bu Pingli Dai |
| author_sort | Ying Lu |
| collection | DOAJ |
| description | Background: Chlorothalonil and acetamiprid are chemical pesticides commonly used in agricultural production and have been shown to have negative effects on bee’s fitness. Despite many studies have revealed that honey bee (Apis mellifera L.) larvae are posting a high risk on exposure to pesticides, but the toxicology information of chlorothalonil and acetamiprid on bee larvae remain limited.Results: The no observed adverse effect concentration (NOAEC) of chlorothalonil and acetamiprid for honey bee larvae were 4 μg/mL and 2 μg/mL, respectively. Except for CarE, the enzymic activities of GST and P450 were not influenced by chlorothalonil at NOAEC, while chronic exposure to acetamiprid slightly increased the activities of the three tested enzymes at NOAEC. Further, the exposed larvae showed significantly higher expression of genes involved in a series of different toxicologically relevant process following, including caste development (Tor (GB44905), InR-2 (GB55425), Hr4 (GB47037), Ac3 (GB11637) and ILP-2 (GB10174)), immune system response (abaecin (GB18323), defensin-1 (GB19392), toll-X4 (GB50418)), and oxidative stress response (P450, GSH, GST, CarE).Conclusion: Our results suggest that the exposure to chlorothalonil and acetamiprid, even at concentrations below the NOAEC, showed potentially effects on bee larvae’s fitness, and more important synergistic and behavioral effects that can affect larvae fitness should be explored in the further. |
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| format | Article |
| id | doaj.art-fbcfec7ddb6b4c7eb98eaf965b866142 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-10T15:37:15Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-fbcfec7ddb6b4c7eb98eaf965b8661422023-02-13T05:12:48ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-02-011410.3389/fphys.2023.11144031114403Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitroYing Lu0Ying Lu1Jing Gao2Tong Wu3Bo Han4Bingnan Qian5Min Shi6Sa Yang7Qingyun Diao8Chunya Bu9Pingli Dai10Key Laboratory of Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing, ChinaKey Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaBackground: Chlorothalonil and acetamiprid are chemical pesticides commonly used in agricultural production and have been shown to have negative effects on bee’s fitness. Despite many studies have revealed that honey bee (Apis mellifera L.) larvae are posting a high risk on exposure to pesticides, but the toxicology information of chlorothalonil and acetamiprid on bee larvae remain limited.Results: The no observed adverse effect concentration (NOAEC) of chlorothalonil and acetamiprid for honey bee larvae were 4 μg/mL and 2 μg/mL, respectively. Except for CarE, the enzymic activities of GST and P450 were not influenced by chlorothalonil at NOAEC, while chronic exposure to acetamiprid slightly increased the activities of the three tested enzymes at NOAEC. Further, the exposed larvae showed significantly higher expression of genes involved in a series of different toxicologically relevant process following, including caste development (Tor (GB44905), InR-2 (GB55425), Hr4 (GB47037), Ac3 (GB11637) and ILP-2 (GB10174)), immune system response (abaecin (GB18323), defensin-1 (GB19392), toll-X4 (GB50418)), and oxidative stress response (P450, GSH, GST, CarE).Conclusion: Our results suggest that the exposure to chlorothalonil and acetamiprid, even at concentrations below the NOAEC, showed potentially effects on bee larvae’s fitness, and more important synergistic and behavioral effects that can affect larvae fitness should be explored in the further.https://www.frontiersin.org/articles/10.3389/fphys.2023.1114403/fullhoney beeschlorothalonilacetamipridtoxicityoxidative stress |
| spellingShingle | Ying Lu Ying Lu Jing Gao Tong Wu Bo Han Bingnan Qian Min Shi Sa Yang Qingyun Diao Chunya Bu Pingli Dai Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro Frontiers in Physiology honey bees chlorothalonil acetamiprid toxicity oxidative stress |
| title | Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro |
| title_full | Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro |
| title_fullStr | Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro |
| title_full_unstemmed | Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro |
| title_short | Exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in Apis mellifera larvae reared in vitro |
| title_sort | exposure of chlorothalonil and acetamiprid reduce the survival and cause multiple internal disturbances in apis mellifera larvae reared in vitro |
| topic | honey bees chlorothalonil acetamiprid toxicity oxidative stress |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1114403/full |
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