C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells.
We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090570&type=printable |
| _version_ | 1826799181482688512 |
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| author | Branko Zevnik Nathalie C Uyttersprot Ana V Perez Gerald W M Bothe Heidrun Kern Gunther Kauselmann |
| author_facet | Branko Zevnik Nathalie C Uyttersprot Ana V Perez Gerald W M Bothe Heidrun Kern Gunther Kauselmann |
| author_sort | Branko Zevnik |
| collection | DOAJ |
| description | We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells. |
| first_indexed | 2024-12-21T03:47:53Z |
| format | Article |
| id | doaj.art-fbd1f6b6d02a40e6b24779b0c8948c6c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-17T00:31:00Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fbd1f6b6d02a40e6b24779b0c8948c6c2025-02-21T05:35:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9057010.1371/journal.pone.0090570C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells.Branko ZevnikNathalie C UyttersprotAna V PerezGerald W M BotheHeidrun KernGunther KauselmannWe generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090570&type=printable |
| spellingShingle | Branko Zevnik Nathalie C Uyttersprot Ana V Perez Gerald W M Bothe Heidrun Kern Gunther Kauselmann C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS ONE |
| title | C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. |
| title_full | C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. |
| title_fullStr | C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. |
| title_full_unstemmed | C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. |
| title_short | C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. |
| title_sort | c57bl 6n albino agouti mutant mice as embryo donors for efficient germline transmission of c57bl 6 es cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090570&type=printable |
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