Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor
Engineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/23/7/3425 |
| _version_ | 1797607089310269440 |
|---|---|
| author | Jana Blaškovičová Vlastimil Vyskočil Michal Augustín Andrea Purdešová |
| author_facet | Jana Blaškovičová Vlastimil Vyskočil Michal Augustín Andrea Purdešová |
| author_sort | Jana Blaškovičová |
| collection | DOAJ |
| description | Engineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological environment of the body containing NaCl, this has raised concerns about the potentially harmful effects of ingested nanomaterials on human health. Although gold nanoparticles (AuNPs) exhibit great potential for various biomedical applications, there is some inconsistency in the case of the unambiguous genotoxicity of AuNPs due to differences in their shape, size, solubility, and exposure time. A DNA/GCE (DNA/glassy carbon electrode) biosensor was used to study ethanol (EtOH) and NaCl-induced gold nanoparticle aggregation genotoxicity under UV light in this study. The genotoxic effect of dispersed and aggregated negatively charged gold nanoparticles AuNP1 (8 nm) and AuNP2 (30 nm) toward salmon sperm double-stranded dsDNA was monitored by cyclic and square-wave voltammetry (CV, SWV). Electrochemical impedance spectroscopy (EIS) was used for a surface study of the biosensor. The aggregation of AuNPs was monitored by UV-vis spectroscopy. AuNP1 aggregates formed by 30% <i>v/v</i> EtOH and 0.15 mol·L<sup>−1</sup> NaCl caused the greatest damage to the biosensor DNA layer. |
| first_indexed | 2024-03-11T05:26:21Z |
| format | Article |
| id | doaj.art-fbd5eceb43c34204ba66a07d70a2dfd2 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T05:26:21Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-fbd5eceb43c34204ba66a07d70a2dfd22023-11-17T17:32:22ZengMDPI AGSensors1424-82202023-03-01237342510.3390/s23073425Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE BiosensorJana Blaškovičová0Vlastimil Vyskočil1Michal Augustín2Andrea Purdešová3Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaUNESCO Laboratory of Environmental Electrochemistry, Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 2030/8, 128 43 Prague, Czech RepublicUNESCO Laboratory of Environmental Electrochemistry, Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 2030/8, 128 43 Prague, Czech RepublicDepartment of Chemistry, Faculty of Natural Sciences, University of Ss. Cyril and Methodius in Trnava, Nám. J. Herdu 2, 917 01 Trnava, SlovakiaEngineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological environment of the body containing NaCl, this has raised concerns about the potentially harmful effects of ingested nanomaterials on human health. Although gold nanoparticles (AuNPs) exhibit great potential for various biomedical applications, there is some inconsistency in the case of the unambiguous genotoxicity of AuNPs due to differences in their shape, size, solubility, and exposure time. A DNA/GCE (DNA/glassy carbon electrode) biosensor was used to study ethanol (EtOH) and NaCl-induced gold nanoparticle aggregation genotoxicity under UV light in this study. The genotoxic effect of dispersed and aggregated negatively charged gold nanoparticles AuNP1 (8 nm) and AuNP2 (30 nm) toward salmon sperm double-stranded dsDNA was monitored by cyclic and square-wave voltammetry (CV, SWV). Electrochemical impedance spectroscopy (EIS) was used for a surface study of the biosensor. The aggregation of AuNPs was monitored by UV-vis spectroscopy. AuNP1 aggregates formed by 30% <i>v/v</i> EtOH and 0.15 mol·L<sup>−1</sup> NaCl caused the greatest damage to the biosensor DNA layer.https://www.mdpi.com/1424-8220/23/7/3425aggregationDNA/GCE biosensorgold nanoparticlestoxicityethanolNaCl |
| spellingShingle | Jana Blaškovičová Vlastimil Vyskočil Michal Augustín Andrea Purdešová Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor Sensors aggregation DNA/GCE biosensor gold nanoparticles toxicity ethanol NaCl |
| title | Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor |
| title_full | Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor |
| title_fullStr | Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor |
| title_full_unstemmed | Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor |
| title_short | Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor |
| title_sort | ethanol and nacl induced gold nanoparticle aggregation toxicity toward dna investigated with a dna gce biosensor |
| topic | aggregation DNA/GCE biosensor gold nanoparticles toxicity ethanol NaCl |
| url | https://www.mdpi.com/1424-8220/23/7/3425 |
| work_keys_str_mv | AT janablaskovicova ethanolandnaclinducedgoldnanoparticleaggregationtoxicitytowarddnainvestigatedwithadnagcebiosensor AT vlastimilvyskocil ethanolandnaclinducedgoldnanoparticleaggregationtoxicitytowarddnainvestigatedwithadnagcebiosensor AT michalaugustin ethanolandnaclinducedgoldnanoparticleaggregationtoxicitytowarddnainvestigatedwithadnagcebiosensor AT andreapurdesova ethanolandnaclinducedgoldnanoparticleaggregationtoxicitytowarddnainvestigatedwithadnagcebiosensor |