Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).

The work was aimed at estimating the detection limits of 21 antibiotics (beta-lactam antibiotics, cephalosporins, aminoglycosides, macrolides and others) by means of three types of detection systems (disk diffusion method with Bacillus stearothermophilus var. calidolactis C 953, Delvotest SP and Pen...

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Main Authors: B. Hozová, M. Kratmüllerová
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2001-08-01
Series:Czech Journal of Food Sciences
Subjects:
Online Access:https://cjfs.agriculturejournals.cz/artkey/cjf-200104-0002_assay-of-antibiotic-detection-limits-in-cow-s-milk-model-samples-and-comparison-of-sensitivity-of-various-detec.php
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author B. Hozová
M. Kratmüllerová
author_facet B. Hozová
M. Kratmüllerová
author_sort B. Hozová
collection DOAJ
description The work was aimed at estimating the detection limits of 21 antibiotics (beta-lactam antibiotics, cephalosporins, aminoglycosides, macrolides and others) by means of three types of detection systems (disk diffusion method with Bacillus stearothermophilus var. calidolactis C 953, Delvotest SP and Penzym S 100) and to verify their sensitivity in the evaluation of the admissible maximum residual limits (MRL). The high sensitivity and the good correlation of results have been achieved mainly by applying the rapid methods such as Delvotest SP and Penzym S 100 versus the less sensitive disk diffusion method. In the next stage of the work, detection limits of the mutual combinations of antibiotics in milk were estimated. In the model experiment, the synergic effect between ampicillin and oxacillin, cefuroxime and trimethoprim and between cephalosporins (combination of cefazolin with cefoperhazon) was observed.
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spelling doaj.art-fbd631f0b7704f7fbbf91211d6ade30a2023-02-23T03:26:31ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172001-08-0119412513110.17221/6595-CJFScjf-200104-0002Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).B. Hozová0M. Kratmüllerová1Slovak University of Technology Faculty of Chemical Technology, Bratislava, Slovak RepublicSlovak University of Technology Faculty of Chemical Technology, Bratislava, Slovak RepublicThe work was aimed at estimating the detection limits of 21 antibiotics (beta-lactam antibiotics, cephalosporins, aminoglycosides, macrolides and others) by means of three types of detection systems (disk diffusion method with Bacillus stearothermophilus var. calidolactis C 953, Delvotest SP and Penzym S 100) and to verify their sensitivity in the evaluation of the admissible maximum residual limits (MRL). The high sensitivity and the good correlation of results have been achieved mainly by applying the rapid methods such as Delvotest SP and Penzym S 100 versus the less sensitive disk diffusion method. In the next stage of the work, detection limits of the mutual combinations of antibiotics in milk were estimated. In the model experiment, the synergic effect between ampicillin and oxacillin, cefuroxime and trimethoprim and between cephalosporins (combination of cefazolin with cefoperhazon) was observed.https://cjfs.agriculturejournals.cz/artkey/cjf-200104-0002_assay-of-antibiotic-detection-limits-in-cow-s-milk-model-samples-and-comparison-of-sensitivity-of-various-detec.phpantibioticsdisk diffusion methoddelvotest sppenzym s 100synergism
spellingShingle B. Hozová
M. Kratmüllerová
Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).
Czech Journal of Food Sciences
antibiotics
disk diffusion method
delvotest sp
penzym s 100
synergism
title Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).
title_full Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).
title_fullStr Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).
title_full_unstemmed Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).
title_short Assay of antibiotic detection limits in cow's milk model samples and comparison of sensitivity of various detection systems (disk diffusion method, Delvotest SP and Penzym S 100).
title_sort assay of antibiotic detection limits in cow s milk model samples and comparison of sensitivity of various detection systems disk diffusion method delvotest sp and penzym s 100
topic antibiotics
disk diffusion method
delvotest sp
penzym s 100
synergism
url https://cjfs.agriculturejournals.cz/artkey/cjf-200104-0002_assay-of-antibiotic-detection-limits-in-cow-s-milk-model-samples-and-comparison-of-sensitivity-of-various-detec.php
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