Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis

ABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divide...

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Main Authors: Ana Carolina Siqueira Gonçalves, Luiz Carlos Roma Júnior, Rafaela Torrezan Privatti, Márcia Saladini Vieira Salles, Claudia Cristina Paro de Paz, Lenira El Faro Zadra, Ana Maria Centola Vidal
Format: Article
Language:English
Published: Universidade Federal de Santa Maria 2018-06-01
Series:Ciência Rural
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600454&lng=en&tlng=en
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author Ana Carolina Siqueira Gonçalves
Luiz Carlos Roma Júnior
Rafaela Torrezan Privatti
Márcia Saladini Vieira Salles
Claudia Cristina Paro de Paz
Lenira El Faro Zadra
Ana Maria Centola Vidal
author_facet Ana Carolina Siqueira Gonçalves
Luiz Carlos Roma Júnior
Rafaela Torrezan Privatti
Márcia Saladini Vieira Salles
Claudia Cristina Paro de Paz
Lenira El Faro Zadra
Ana Maria Centola Vidal
author_sort Ana Carolina Siqueira Gonçalves
collection DOAJ
description ABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.
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spelling doaj.art-fbd699b43721475bbd98172b5f0ce2d82022-12-21T23:05:28ZengUniversidade Federal de Santa MariaCiência Rural1678-45962018-06-0148610.1590/0103-8478cr20170848S0103-84782018000600454Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysisAna Carolina Siqueira GonçalvesLuiz Carlos Roma JúniorRafaela Torrezan PrivattiMárcia Saladini Vieira SallesClaudia Cristina Paro de PazLenira El Faro ZadraAna Maria Centola VidalABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600454&lng=en&tlng=enmonitoringudder healthalternative methodsomatic cell count
spellingShingle Ana Carolina Siqueira Gonçalves
Luiz Carlos Roma Júnior
Rafaela Torrezan Privatti
Márcia Saladini Vieira Salles
Claudia Cristina Paro de Paz
Lenira El Faro Zadra
Ana Maria Centola Vidal
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis
Ciência Rural
monitoring
udder health
alternative method
somatic cell count
title Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis
title_full Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis
title_fullStr Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis
title_full_unstemmed Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis
title_short Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis
title_sort somatic cell count obtained by ekomilk scan r and correlations with other methods of analysis
topic monitoring
udder health
alternative method
somatic cell count
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600454&lng=en&tlng=en
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