Analysis of Differentially Expressed Metabolites of Fresh and Dried Wolfberry Fruit in Ningxia Based on UPLC-QE-Orbitrap-MS
The project studied the differences of dried and fresh wolfberry fruit in Ningxia region. Using non-targeted metabolomics analysed the differential metabolites of wolfberry fruit. The observed variables were determined using ultra-high performance liquid chromatography-quadrupole-electrostatic field...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-04-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080184 |
| _version_ | 1827959301977669632 |
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| author | Lihua TANG Yao ZHANG Xuemei MA Guijuan MA Jianxing YANG |
| author_facet | Lihua TANG Yao ZHANG Xuemei MA Guijuan MA Jianxing YANG |
| author_sort | Lihua TANG |
| collection | DOAJ |
| description | The project studied the differences of dried and fresh wolfberry fruit in Ningxia region. Using non-targeted metabolomics analysed the differential metabolites of wolfberry fruit. The observed variables were determined using ultra-high performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry (UPLC-QE-Orbitrap-MS). Differential metabolites were screened out by mathematical statistical methods (principal component analysis (PCA), partial least squares analysis (PLS-DA), univariate statistical analysis, cluster analysis, volcano plot) to identify and analyse all metabolites. The results showed that 35 different metabolites were identified in the fresh and dried wolfberry fruit. The expression of organic acids, amino acids and flavonoids was up-regulated, while the expression of nucleotides, phenolic acids and coumarin compounds was down-regulated. This study revealed the characteristic compounds of fresh and dried wolfberry fruit in Ningxia. The research would provide a theoretical basis for the material basis of different wolfberry fruits. |
| first_indexed | 2024-04-09T15:49:07Z |
| format | Article |
| id | doaj.art-fbd6e1e0a1ad4d4591eea2b3bfb02fa3 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-09T15:49:07Z |
| publishDate | 2023-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-fbd6e1e0a1ad4d4591eea2b3bfb02fa32023-04-26T12:48:50ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-04-0144891610.13386/j.issn1002-0306.20220801842022080184-8Analysis of Differentially Expressed Metabolites of Fresh and Dried Wolfberry Fruit in Ningxia Based on UPLC-QE-Orbitrap-MSLihua TANG0Yao ZHANG1Xuemei MA2Guijuan MA3Jianxing YANG4Ningxia Food Testing and Research Institute (Key Laboratory of Quality and Safety of Wolfberry and Wine for State Administration for Market Regulation), Yinchuan 750001, ChinaNingxia Food Testing and Research Institute (Key Laboratory of Quality and Safety of Wolfberry and Wine for State Administration for Market Regulation), Yinchuan 750001, ChinaNingxia Food Testing and Research Institute (Key Laboratory of Quality and Safety of Wolfberry and Wine for State Administration for Market Regulation), Yinchuan 750001, ChinaNingxia Food Testing and Research Institute (Key Laboratory of Quality and Safety of Wolfberry and Wine for State Administration for Market Regulation), Yinchuan 750001, ChinaNingxia Food Testing and Research Institute (Key Laboratory of Quality and Safety of Wolfberry and Wine for State Administration for Market Regulation), Yinchuan 750001, ChinaThe project studied the differences of dried and fresh wolfberry fruit in Ningxia region. Using non-targeted metabolomics analysed the differential metabolites of wolfberry fruit. The observed variables were determined using ultra-high performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry (UPLC-QE-Orbitrap-MS). Differential metabolites were screened out by mathematical statistical methods (principal component analysis (PCA), partial least squares analysis (PLS-DA), univariate statistical analysis, cluster analysis, volcano plot) to identify and analyse all metabolites. The results showed that 35 different metabolites were identified in the fresh and dried wolfberry fruit. The expression of organic acids, amino acids and flavonoids was up-regulated, while the expression of nucleotides, phenolic acids and coumarin compounds was down-regulated. This study revealed the characteristic compounds of fresh and dried wolfberry fruit in Ningxia. The research would provide a theoretical basis for the material basis of different wolfberry fruits.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080184fresh wolfberrydried wolfberryuntargeted metabolomicsdifferential metabolites |
| spellingShingle | Lihua TANG Yao ZHANG Xuemei MA Guijuan MA Jianxing YANG Analysis of Differentially Expressed Metabolites of Fresh and Dried Wolfberry Fruit in Ningxia Based on UPLC-QE-Orbitrap-MS Shipin gongye ke-ji fresh wolfberry dried wolfberry untargeted metabolomics differential metabolites |
| title | Analysis of Differentially Expressed Metabolites of Fresh and Dried Wolfberry Fruit in Ningxia Based on UPLC-QE-Orbitrap-MS |
| title_full | Analysis of Differentially Expressed Metabolites of Fresh and Dried Wolfberry Fruit in Ningxia Based on UPLC-QE-Orbitrap-MS |
| title_fullStr | Analysis of Differentially Expressed Metabolites of Fresh and Dried Wolfberry Fruit in Ningxia Based on UPLC-QE-Orbitrap-MS |
| title_full_unstemmed | Analysis of Differentially Expressed Metabolites of Fresh and Dried Wolfberry Fruit in Ningxia Based on UPLC-QE-Orbitrap-MS |
| title_short | Analysis of Differentially Expressed Metabolites of Fresh and Dried Wolfberry Fruit in Ningxia Based on UPLC-QE-Orbitrap-MS |
| title_sort | analysis of differentially expressed metabolites of fresh and dried wolfberry fruit in ningxia based on uplc qe orbitrap ms |
| topic | fresh wolfberry dried wolfberry untargeted metabolomics differential metabolites |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080184 |
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