1H-NMR metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or Corexit
We used proton nuclear magnetic resonance spectroscopy (1H-NMR) to evaluate metabolic impacts of environmentally relevant crude oil and Corexit exposures on the physiology of hatchling loggerhead sea turtles (Caretta caretta). Sample extraction and data acquisition methods for very small volume whol...
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| Format: | Article |
| Language: | English |
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The Royal Society
2017-01-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171433 |
| _version_ | 1819136129397424128 |
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| author | Stasia A. Bembenek Bailey Jennifer N. Niemuth Patricia D. McClellan-Green Matthew H. Godfrey Craig A. Harms Michael K. Stoskopf |
| author_facet | Stasia A. Bembenek Bailey Jennifer N. Niemuth Patricia D. McClellan-Green Matthew H. Godfrey Craig A. Harms Michael K. Stoskopf |
| author_sort | Stasia A. Bembenek Bailey |
| collection | DOAJ |
| description | We used proton nuclear magnetic resonance spectroscopy (1H-NMR) to evaluate metabolic impacts of environmentally relevant crude oil and Corexit exposures on the physiology of hatchling loggerhead sea turtles (Caretta caretta). Sample extraction and data acquisition methods for very small volume whole blood samples and sources of variation between individual hatchlings were assessed. Sixteen unclotted, whole blood samples were obtained from 7-day-old hatchlings after a 4-day cutaneous exposure to either control seawater, crude oil, Corexit 9500A or a combination of crude oil and Corexit 9500A. After extraction, one- and two-dimensional 1H-NMR spectra of the samples were obtained, and 17 metabolites were identified and confirmed in the whole blood spectra. Variation among samples due to the concentrations of metabolites 3-hydroxybutyrate, lactate, trimethylamine oxide and propylene glycol did not statistically correlate with treatment group. However, the characterization of the hatchling loggerhead whole blood metabolome provides a foundation for future metabolomic research with sea turtles and a basis for the study of tissues from exposed hatchling sea turtles. |
| first_indexed | 2024-12-22T10:30:04Z |
| format | Article |
| id | doaj.art-fbdcdb7d74cf47bcbd84c16df6c48abc |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-22T10:30:04Z |
| publishDate | 2017-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-fbdcdb7d74cf47bcbd84c16df6c48abc2022-12-21T18:29:21ZengThe Royal SocietyRoyal Society Open Science2054-57032017-01-0141110.1098/rsos.1714331714331H-NMR metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or CorexitStasia A. Bembenek BaileyJennifer N. NiemuthPatricia D. McClellan-GreenMatthew H. GodfreyCraig A. HarmsMichael K. StoskopfWe used proton nuclear magnetic resonance spectroscopy (1H-NMR) to evaluate metabolic impacts of environmentally relevant crude oil and Corexit exposures on the physiology of hatchling loggerhead sea turtles (Caretta caretta). Sample extraction and data acquisition methods for very small volume whole blood samples and sources of variation between individual hatchlings were assessed. Sixteen unclotted, whole blood samples were obtained from 7-day-old hatchlings after a 4-day cutaneous exposure to either control seawater, crude oil, Corexit 9500A or a combination of crude oil and Corexit 9500A. After extraction, one- and two-dimensional 1H-NMR spectra of the samples were obtained, and 17 metabolites were identified and confirmed in the whole blood spectra. Variation among samples due to the concentrations of metabolites 3-hydroxybutyrate, lactate, trimethylamine oxide and propylene glycol did not statistically correlate with treatment group. However, the characterization of the hatchling loggerhead whole blood metabolome provides a foundation for future metabolomic research with sea turtles and a basis for the study of tissues from exposed hatchling sea turtles.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171433caretta carettacorexit 9500acrude oilnuclear magnetic resonance spectroscopysmall volume sampleswhole blood |
| spellingShingle | Stasia A. Bembenek Bailey Jennifer N. Niemuth Patricia D. McClellan-Green Matthew H. Godfrey Craig A. Harms Michael K. Stoskopf 1H-NMR metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or Corexit Royal Society Open Science caretta caretta corexit 9500a crude oil nuclear magnetic resonance spectroscopy small volume samples whole blood |
| title | 1H-NMR metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or Corexit |
| title_full | 1H-NMR metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or Corexit |
| title_fullStr | 1H-NMR metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or Corexit |
| title_full_unstemmed | 1H-NMR metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or Corexit |
| title_short | 1H-NMR metabolomic study of whole blood from hatchling loggerhead sea turtles (Caretta caretta) exposed to crude oil and/or Corexit |
| title_sort | 1h nmr metabolomic study of whole blood from hatchling loggerhead sea turtles caretta caretta exposed to crude oil and or corexit |
| topic | caretta caretta corexit 9500a crude oil nuclear magnetic resonance spectroscopy small volume samples whole blood |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171433 |
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