Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia
Abstract microRNAs (miRNAs) play important roles in regulation of gene expression during cervical carcinogenesis. We investigated expression profiles of miRNAs in cervical cancer and its precursor lesions by utilizing cervical mucus. Cervical mucus was collected from 230 patients with a normal cervi...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-05-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-25310-1 |
| _version_ | 1818428223506087936 |
|---|---|
| author | Satoshi Kawai Takuma Fujii Iwao Kukimoto Hiroya Yamada Naoki Yamamoto Makoto Kuroda Sayaka Otani Ryoko Ichikawa Eiji Nishio Yutaka Torii Aya Iwata |
| author_facet | Satoshi Kawai Takuma Fujii Iwao Kukimoto Hiroya Yamada Naoki Yamamoto Makoto Kuroda Sayaka Otani Ryoko Ichikawa Eiji Nishio Yutaka Torii Aya Iwata |
| author_sort | Satoshi Kawai |
| collection | DOAJ |
| description | Abstract microRNAs (miRNAs) play important roles in regulation of gene expression during cervical carcinogenesis. We investigated expression profiles of miRNAs in cervical cancer and its precursor lesions by utilizing cervical mucus. Cervical mucus was collected from 230 patients with a normal cervix, cervical intraepithelial neoplasia (CIN), squamous cell carcinoma (SCC), or adenocarcinoma (AD). The levels of miRNA in the mucus were quantified by miRNA array and real-time reverse-transcriptase polymerase chain reaction (RT-PCR). The performance for detecting diseases was statistically analysed. The expression of miRNAs was further validated in the surgical tissues of enrolled patients. Four miRNAs (miR-126-3p, -20b-5p, -451a, and -144-3p) were significantly up-regulated in SCC and AD compared with normal, and their expression levels correlated with disease severity and high-risk human papillomavirus infection. Receiver operating characteristic curve analyses revealed that the area under the curve values for miR-126-3p, -20b-5p, -451a, and -144-3p were 0.89, 0.90, 0.94, and 0.93, respectively, for SCC plus AD compared with normal, showing high accuracy of cancer detection. Real-time RT-PCR analyses confirmed the expression of these four miRNAs in frozen tissues from cervical cancer. miR-126-3p, -20b-5p, -451a, and -144-3p in cervical mucus are promising biomarkers for cervical cancer and high-grade CINs. |
| first_indexed | 2024-12-14T14:58:12Z |
| format | Article |
| id | doaj.art-fbde035407b74b598073ab70845d7a12 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:58:12Z |
| publishDate | 2018-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fbde035407b74b598073ab70845d7a122022-12-21T22:56:57ZengNature PortfolioScientific Reports2045-23222018-05-018111110.1038/s41598-018-25310-1Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasiaSatoshi Kawai0Takuma Fujii1Iwao Kukimoto2Hiroya Yamada3Naoki Yamamoto4Makoto Kuroda5Sayaka Otani6Ryoko Ichikawa7Eiji Nishio8Yutaka Torii9Aya Iwata10Department of Obstetrics and Gynecology, Fujita Health University, School of MedicineDepartment of Obstetrics and Gynecology, Fujita Health University, School of MedicinePathogen Genomics Center, National Institute of Infectious Diseases, Musashi-murayamaDepartment of Hygiene, Fujita Health University, School of MedicineLaboratory of Molecular Biology and Histochemistry Joint Research Support Promotion Facility Center for Research Promotion and Support, Fujita Health University, School of MedicineDepartment of Pathology, Fujita Health University, School of MedicineDepartment of Obstetrics and Gynecology, Fujita Health University, School of MedicineDepartment of Obstetrics and Gynecology, Fujita Health University, School of MedicineDepartment of Obstetrics and Gynecology, Fujita Health University, School of MedicineDepartment of Obstetrics and Gynecology, Fujita Health University, School of MedicineDepartment of Obstetrics and Gynecology, Fujita Health University, School of MedicineAbstract microRNAs (miRNAs) play important roles in regulation of gene expression during cervical carcinogenesis. We investigated expression profiles of miRNAs in cervical cancer and its precursor lesions by utilizing cervical mucus. Cervical mucus was collected from 230 patients with a normal cervix, cervical intraepithelial neoplasia (CIN), squamous cell carcinoma (SCC), or adenocarcinoma (AD). The levels of miRNA in the mucus were quantified by miRNA array and real-time reverse-transcriptase polymerase chain reaction (RT-PCR). The performance for detecting diseases was statistically analysed. The expression of miRNAs was further validated in the surgical tissues of enrolled patients. Four miRNAs (miR-126-3p, -20b-5p, -451a, and -144-3p) were significantly up-regulated in SCC and AD compared with normal, and their expression levels correlated with disease severity and high-risk human papillomavirus infection. Receiver operating characteristic curve analyses revealed that the area under the curve values for miR-126-3p, -20b-5p, -451a, and -144-3p were 0.89, 0.90, 0.94, and 0.93, respectively, for SCC plus AD compared with normal, showing high accuracy of cancer detection. Real-time RT-PCR analyses confirmed the expression of these four miRNAs in frozen tissues from cervical cancer. miR-126-3p, -20b-5p, -451a, and -144-3p in cervical mucus are promising biomarkers for cervical cancer and high-grade CINs.https://doi.org/10.1038/s41598-018-25310-1 |
| spellingShingle | Satoshi Kawai Takuma Fujii Iwao Kukimoto Hiroya Yamada Naoki Yamamoto Makoto Kuroda Sayaka Otani Ryoko Ichikawa Eiji Nishio Yutaka Torii Aya Iwata Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia Scientific Reports |
| title | Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia |
| title_full | Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia |
| title_fullStr | Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia |
| title_full_unstemmed | Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia |
| title_short | Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia |
| title_sort | identification of mirnas in cervical mucus as a novel diagnostic marker for cervical neoplasia |
| url | https://doi.org/10.1038/s41598-018-25310-1 |
| work_keys_str_mv | AT satoshikawai identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT takumafujii identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT iwaokukimoto identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT hiroyayamada identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT naokiyamamoto identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT makotokuroda identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT sayakaotani identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT ryokoichikawa identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT eijinishio identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT yutakatorii identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia AT ayaiwata identificationofmirnasincervicalmucusasanoveldiagnosticmarkerforcervicalneoplasia |