Hypoxia Induces Autophagy of Bone Marrow-Derived Mesenchymal Stem Cells via Activation of ERK1/2
Background: Bone marrow-derived mesenchymal stem cells (bmMSCs) are the most promising seed cells for cell transplant therapy. Hypoxia is a known stimulus of autophagy. Recent studies showed that hypoxia promotes autophagy of human placental chorionic plate-derived mesenchymal stem cells (CP-MSCs)....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2014-05-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/358711 |
| _version_ | 1819050520867766272 |
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| author | Junfang Wu Jie Niu Xiaopeng Li Yonghai Li Xianwei Wang Juntang Lin Fenxi Zhang |
| author_facet | Junfang Wu Jie Niu Xiaopeng Li Yonghai Li Xianwei Wang Juntang Lin Fenxi Zhang |
| author_sort | Junfang Wu |
| collection | DOAJ |
| description | Background: Bone marrow-derived mesenchymal stem cells (bmMSCs) are the most promising seed cells for cell transplant therapy. Hypoxia is a known stimulus of autophagy. Recent studies showed that hypoxia promotes autophagy of human placental chorionic plate-derived mesenchymal stem cells (CP-MSCs). However, whether hypoxia affects autophagy of bmMSCs has not been examined. The goal of this study was to investigate the effect of hypoxia on autophagy of mouse bmMSCs and the underlying mechanisms. Methods: BmMSCs from mouse bone marrow were randomly divided into three groups: control (C), hypoxia (H) and hypoxia + reoxygenation (H+R) groups. Subsequent autophagic signals were analyzed by immunostaining and Western blot assays. Results: The expression of autophagic signals LC-3, Atg5 and Beclin-1, as well as the conversion of LC3B-I to LC3B-II in bmMSCs were significantly increased in H group as compared with control (pConclusion: Hypoxia can induce autophagy of bmMSCs, which depends on activation of ERK1/2 pathway. |
| first_indexed | 2024-12-21T11:49:21Z |
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| id | doaj.art-fbdee9bbc14647538604bd69bafcddbd |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-21T11:49:21Z |
| publishDate | 2014-05-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-fbdee9bbc14647538604bd69bafcddbd2022-12-21T19:05:06ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-05-013351467147410.1159/000358711358711Hypoxia Induces Autophagy of Bone Marrow-Derived Mesenchymal Stem Cells via Activation of ERK1/2Junfang WuJie NiuXiaopeng LiYonghai LiXianwei WangJuntang LinFenxi ZhangBackground: Bone marrow-derived mesenchymal stem cells (bmMSCs) are the most promising seed cells for cell transplant therapy. Hypoxia is a known stimulus of autophagy. Recent studies showed that hypoxia promotes autophagy of human placental chorionic plate-derived mesenchymal stem cells (CP-MSCs). However, whether hypoxia affects autophagy of bmMSCs has not been examined. The goal of this study was to investigate the effect of hypoxia on autophagy of mouse bmMSCs and the underlying mechanisms. Methods: BmMSCs from mouse bone marrow were randomly divided into three groups: control (C), hypoxia (H) and hypoxia + reoxygenation (H+R) groups. Subsequent autophagic signals were analyzed by immunostaining and Western blot assays. Results: The expression of autophagic signals LC-3, Atg5 and Beclin-1, as well as the conversion of LC3B-I to LC3B-II in bmMSCs were significantly increased in H group as compared with control (pConclusion: Hypoxia can induce autophagy of bmMSCs, which depends on activation of ERK1/2 pathway.http://www.karger.com/Article/FullText/358711HypoxiaBone marrow derived mesenchymal stem cellsExtracellular signal-regulated kinase 1/2 (ERK1/2)Autophagy |
| spellingShingle | Junfang Wu Jie Niu Xiaopeng Li Yonghai Li Xianwei Wang Juntang Lin Fenxi Zhang Hypoxia Induces Autophagy of Bone Marrow-Derived Mesenchymal Stem Cells via Activation of ERK1/2 Cellular Physiology and Biochemistry Hypoxia Bone marrow derived mesenchymal stem cells Extracellular signal-regulated kinase 1/2 (ERK1/2) Autophagy |
| title | Hypoxia Induces Autophagy of Bone Marrow-Derived Mesenchymal Stem Cells via Activation of ERK1/2 |
| title_full | Hypoxia Induces Autophagy of Bone Marrow-Derived Mesenchymal Stem Cells via Activation of ERK1/2 |
| title_fullStr | Hypoxia Induces Autophagy of Bone Marrow-Derived Mesenchymal Stem Cells via Activation of ERK1/2 |
| title_full_unstemmed | Hypoxia Induces Autophagy of Bone Marrow-Derived Mesenchymal Stem Cells via Activation of ERK1/2 |
| title_short | Hypoxia Induces Autophagy of Bone Marrow-Derived Mesenchymal Stem Cells via Activation of ERK1/2 |
| title_sort | hypoxia induces autophagy of bone marrow derived mesenchymal stem cells via activation of erk1 2 |
| topic | Hypoxia Bone marrow derived mesenchymal stem cells Extracellular signal-regulated kinase 1/2 (ERK1/2) Autophagy |
| url | http://www.karger.com/Article/FullText/358711 |
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