Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation

Abstract Background Microbial communities harbor important biotechnological potential in diverse domains, however, the engineering and propagation of such communities still face both knowledge and know-how gaps. More specifically, culturing tools are needed to propagate and shape microbial communiti...

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Main Authors: Alexis Dijamentiuk, Cécile Mangavel, Chloé Gapp, Annelore Elfassy, Anne-Marie Revol-Junelles, Frédéric Borges
Format: Article
Language:English
Published: BMC 2024-02-01
Series:Microbial Cell Factories
Subjects:
Online Access:https://doi.org/10.1186/s12934-024-02322-3
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author Alexis Dijamentiuk
Cécile Mangavel
Chloé Gapp
Annelore Elfassy
Anne-Marie Revol-Junelles
Frédéric Borges
author_facet Alexis Dijamentiuk
Cécile Mangavel
Chloé Gapp
Annelore Elfassy
Anne-Marie Revol-Junelles
Frédéric Borges
author_sort Alexis Dijamentiuk
collection DOAJ
description Abstract Background Microbial communities harbor important biotechnological potential in diverse domains, however, the engineering and propagation of such communities still face both knowledge and know-how gaps. More specifically, culturing tools are needed to propagate and shape microbial communities, to obtain desired properties, and to exploit them. Previous work suggested that micro-confinement and segregation of microorganisms using invert (water-in-oil, w/o) emulsion broth can shape communities during propagation, by alleviating biotic interactions and inducing physiological changes in cultured bacteria. The present work aimed at evaluating invert emulsion and simple broth monophasic cultures for the propagation and shaping of bacterial communities derived from raw milk in a serial propagation design. Results The monophasic setup resulted in stable community structures during serial propagation, whereas the invert emulsion system resulted in only transiently stable structures. In addition, different communities with different taxonomic compositions could be obtained from a single inoculum. Furthermore, the implementation of invert emulsion systems has allowed for the enrichment of less abundant microorganisms and consequently facilitated their isolation on culture agar plates. Conclusions The monophasic system enables communities to be propagated in a stable manner, whereas the invert emulsion system allowed for the isolation of less abundant microorganisms and the generation of diverse taxonomic compositions from a single inoculum.
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spelling doaj.art-fbdefd0e74144885b19c000a462e23bb2024-03-06T08:06:23ZengBMCMicrobial Cell Factories1475-28592024-02-0123111210.1186/s12934-024-02322-3Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagationAlexis Dijamentiuk0Cécile Mangavel1Chloé Gapp2Annelore Elfassy3Anne-Marie Revol-Junelles4Frédéric Borges5Laboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineAbstract Background Microbial communities harbor important biotechnological potential in diverse domains, however, the engineering and propagation of such communities still face both knowledge and know-how gaps. More specifically, culturing tools are needed to propagate and shape microbial communities, to obtain desired properties, and to exploit them. Previous work suggested that micro-confinement and segregation of microorganisms using invert (water-in-oil, w/o) emulsion broth can shape communities during propagation, by alleviating biotic interactions and inducing physiological changes in cultured bacteria. The present work aimed at evaluating invert emulsion and simple broth monophasic cultures for the propagation and shaping of bacterial communities derived from raw milk in a serial propagation design. Results The monophasic setup resulted in stable community structures during serial propagation, whereas the invert emulsion system resulted in only transiently stable structures. In addition, different communities with different taxonomic compositions could be obtained from a single inoculum. Furthermore, the implementation of invert emulsion systems has allowed for the enrichment of less abundant microorganisms and consequently facilitated their isolation on culture agar plates. Conclusions The monophasic system enables communities to be propagated in a stable manner, whereas the invert emulsion system allowed for the isolation of less abundant microorganisms and the generation of diverse taxonomic compositions from a single inoculum.https://doi.org/10.1186/s12934-024-02322-3Serial passagingMicrobial communityCoculture systemsMicrobiome engineeringCommunity structureEnrichment culture
spellingShingle Alexis Dijamentiuk
Cécile Mangavel
Chloé Gapp
Annelore Elfassy
Anne-Marie Revol-Junelles
Frédéric Borges
Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation
Microbial Cell Factories
Serial passaging
Microbial community
Coculture systems
Microbiome engineering
Community structure
Enrichment culture
title Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation
title_full Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation
title_fullStr Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation
title_full_unstemmed Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation
title_short Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation
title_sort serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation
topic Serial passaging
Microbial community
Coculture systems
Microbiome engineering
Community structure
Enrichment culture
url https://doi.org/10.1186/s12934-024-02322-3
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