Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation
Abstract Background Microbial communities harbor important biotechnological potential in diverse domains, however, the engineering and propagation of such communities still face both knowledge and know-how gaps. More specifically, culturing tools are needed to propagate and shape microbial communiti...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2024-02-01
|
Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12934-024-02322-3 |
_version_ | 1797272325449580544 |
---|---|
author | Alexis Dijamentiuk Cécile Mangavel Chloé Gapp Annelore Elfassy Anne-Marie Revol-Junelles Frédéric Borges |
author_facet | Alexis Dijamentiuk Cécile Mangavel Chloé Gapp Annelore Elfassy Anne-Marie Revol-Junelles Frédéric Borges |
author_sort | Alexis Dijamentiuk |
collection | DOAJ |
description | Abstract Background Microbial communities harbor important biotechnological potential in diverse domains, however, the engineering and propagation of such communities still face both knowledge and know-how gaps. More specifically, culturing tools are needed to propagate and shape microbial communities, to obtain desired properties, and to exploit them. Previous work suggested that micro-confinement and segregation of microorganisms using invert (water-in-oil, w/o) emulsion broth can shape communities during propagation, by alleviating biotic interactions and inducing physiological changes in cultured bacteria. The present work aimed at evaluating invert emulsion and simple broth monophasic cultures for the propagation and shaping of bacterial communities derived from raw milk in a serial propagation design. Results The monophasic setup resulted in stable community structures during serial propagation, whereas the invert emulsion system resulted in only transiently stable structures. In addition, different communities with different taxonomic compositions could be obtained from a single inoculum. Furthermore, the implementation of invert emulsion systems has allowed for the enrichment of less abundant microorganisms and consequently facilitated their isolation on culture agar plates. Conclusions The monophasic system enables communities to be propagated in a stable manner, whereas the invert emulsion system allowed for the isolation of less abundant microorganisms and the generation of diverse taxonomic compositions from a single inoculum. |
first_indexed | 2024-03-07T14:25:46Z |
format | Article |
id | doaj.art-fbdefd0e74144885b19c000a462e23bb |
institution | Directory Open Access Journal |
issn | 1475-2859 |
language | English |
last_indexed | 2024-03-07T14:25:46Z |
publishDate | 2024-02-01 |
publisher | BMC |
record_format | Article |
series | Microbial Cell Factories |
spelling | doaj.art-fbdefd0e74144885b19c000a462e23bb2024-03-06T08:06:23ZengBMCMicrobial Cell Factories1475-28592024-02-0123111210.1186/s12934-024-02322-3Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagationAlexis Dijamentiuk0Cécile Mangavel1Chloé Gapp2Annelore Elfassy3Anne-Marie Revol-Junelles4Frédéric Borges5Laboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de LorraineAbstract Background Microbial communities harbor important biotechnological potential in diverse domains, however, the engineering and propagation of such communities still face both knowledge and know-how gaps. More specifically, culturing tools are needed to propagate and shape microbial communities, to obtain desired properties, and to exploit them. Previous work suggested that micro-confinement and segregation of microorganisms using invert (water-in-oil, w/o) emulsion broth can shape communities during propagation, by alleviating biotic interactions and inducing physiological changes in cultured bacteria. The present work aimed at evaluating invert emulsion and simple broth monophasic cultures for the propagation and shaping of bacterial communities derived from raw milk in a serial propagation design. Results The monophasic setup resulted in stable community structures during serial propagation, whereas the invert emulsion system resulted in only transiently stable structures. In addition, different communities with different taxonomic compositions could be obtained from a single inoculum. Furthermore, the implementation of invert emulsion systems has allowed for the enrichment of less abundant microorganisms and consequently facilitated their isolation on culture agar plates. Conclusions The monophasic system enables communities to be propagated in a stable manner, whereas the invert emulsion system allowed for the isolation of less abundant microorganisms and the generation of diverse taxonomic compositions from a single inoculum.https://doi.org/10.1186/s12934-024-02322-3Serial passagingMicrobial communityCoculture systemsMicrobiome engineeringCommunity structureEnrichment culture |
spellingShingle | Alexis Dijamentiuk Cécile Mangavel Chloé Gapp Annelore Elfassy Anne-Marie Revol-Junelles Frédéric Borges Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation Microbial Cell Factories Serial passaging Microbial community Coculture systems Microbiome engineering Community structure Enrichment culture |
title | Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation |
title_full | Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation |
title_fullStr | Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation |
title_full_unstemmed | Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation |
title_short | Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation |
title_sort | serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation |
topic | Serial passaging Microbial community Coculture systems Microbiome engineering Community structure Enrichment culture |
url | https://doi.org/10.1186/s12934-024-02322-3 |
work_keys_str_mv | AT alexisdijamentiuk serialculturesininvertemulsionandmonophasesystemsformicrobialcommunityshapingandpropagation AT cecilemangavel serialculturesininvertemulsionandmonophasesystemsformicrobialcommunityshapingandpropagation AT chloegapp serialculturesininvertemulsionandmonophasesystemsformicrobialcommunityshapingandpropagation AT anneloreelfassy serialculturesininvertemulsionandmonophasesystemsformicrobialcommunityshapingandpropagation AT annemarierevoljunelles serialculturesininvertemulsionandmonophasesystemsformicrobialcommunityshapingandpropagation AT fredericborges serialculturesininvertemulsionandmonophasesystemsformicrobialcommunityshapingandpropagation |