Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital
Cervical cancer is the second most common cancer among women in the world. Persistent infection with high risk human papillomavirus (HPV) is one of the necessary causes of cervical cancer development. However, host genetic factors may also play a role in cervical cancer carcinogenesis. Methylenetetr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universitas Padjajaran
2016-12-01
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| Series: | Majalah Kedokteran Bandung |
| Subjects: | |
| Online Access: | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/754 |
| _version_ | 1819154901635170304 |
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| author | Ani Melani Maskoen Cynthia Kurniawan Herman Susanto Edhyana Sahiratmadja |
| author_facet | Ani Melani Maskoen Cynthia Kurniawan Herman Susanto Edhyana Sahiratmadja |
| author_sort | Ani Melani Maskoen |
| collection | DOAJ |
| description | Cervical cancer is the second most common cancer among women in the world. Persistent infection with high risk human papillomavirus (HPV) is one of the necessary causes of cervical cancer development. However, host genetic factors may also play a role in cervical cancer carcinogenesis. Methylenetetrahydrofolate reductase enzyme, encoded by the MTHFR gene, regulates folate metabolism which is important for genetic expression and stability. Single nucleotide polymorphism (SNP) C677T in MTHFR gene may produce a thermo-labile enzyme, resulting in a reduced enzyme activity. The aim of this study was to explore the SNP C677T of MTHFR gene and the susceptibility to cervical cancer among cancer patients visiting Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. This descriptive quantitative study involved cervical cancer patients recruited in 2010 and their control group. Genomic DNA was extracted from patients’ blood. MTHFR C677T genotype was performed using BeadXpress Reader Illumina® and some samples were re-genotyped for confirmation using conventional PCR-RFLP. The distribution of MTHFR C677T genotype in cervical cancer patients was 71.6%, 25.4%, and 3%, and 44%, 36%, and 20% in control group for CC, CT, and TT, respectively. This yielded a statistical significant difference of CC vs CT+TT (p 0.014 with OR 3.22 and CI 95% 1.24 – 8.33). Taken together, this result indicates that T allele has a protective effect against cervical cancer development. Further studies to confirm this effect in bigger population is warranted. [MKB. 2016;48(4):216–21] |
| first_indexed | 2024-12-22T15:28:27Z |
| format | Article |
| id | doaj.art-fbe20e7cd320434eab04e37ac10c723f |
| institution | Directory Open Access Journal |
| issn | 0126-074X 2338-6223 |
| language | English |
| last_indexed | 2024-12-22T15:28:27Z |
| publishDate | 2016-12-01 |
| publisher | Universitas Padjajaran |
| record_format | Article |
| series | Majalah Kedokteran Bandung |
| spelling | doaj.art-fbe20e7cd320434eab04e37ac10c723f2022-12-21T18:21:26ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232016-12-01484216221Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General HospitalAni Melani MaskoenCynthia KurniawanHerman SusantoEdhyana SahiratmadjaCervical cancer is the second most common cancer among women in the world. Persistent infection with high risk human papillomavirus (HPV) is one of the necessary causes of cervical cancer development. However, host genetic factors may also play a role in cervical cancer carcinogenesis. Methylenetetrahydrofolate reductase enzyme, encoded by the MTHFR gene, regulates folate metabolism which is important for genetic expression and stability. Single nucleotide polymorphism (SNP) C677T in MTHFR gene may produce a thermo-labile enzyme, resulting in a reduced enzyme activity. The aim of this study was to explore the SNP C677T of MTHFR gene and the susceptibility to cervical cancer among cancer patients visiting Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. This descriptive quantitative study involved cervical cancer patients recruited in 2010 and their control group. Genomic DNA was extracted from patients’ blood. MTHFR C677T genotype was performed using BeadXpress Reader Illumina® and some samples were re-genotyped for confirmation using conventional PCR-RFLP. The distribution of MTHFR C677T genotype in cervical cancer patients was 71.6%, 25.4%, and 3%, and 44%, 36%, and 20% in control group for CC, CT, and TT, respectively. This yielded a statistical significant difference of CC vs CT+TT (p 0.014 with OR 3.22 and CI 95% 1.24 – 8.33). Taken together, this result indicates that T allele has a protective effect against cervical cancer development. Further studies to confirm this effect in bigger population is warranted. [MKB. 2016;48(4):216–21]http://journal.fk.unpad.ac.id/index.php/mkb/article/view/754MTHFR C677TpolymorphismBandungCervical cancerBandung |
| spellingShingle | Ani Melani Maskoen Cynthia Kurniawan Herman Susanto Edhyana Sahiratmadja Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital Majalah Kedokteran Bandung MTHFR C677T polymorphism Bandung Cervical cancer Bandung |
| title | Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital |
| title_full | Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital |
| title_fullStr | Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital |
| title_full_unstemmed | Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital |
| title_short | Methylenetetrahydrofolate Reductase C677T Distribution among Cervical Cancer Patients at Dr. Hasan Sadikin General Hospital |
| title_sort | methylenetetrahydrofolate reductase c677t distribution among cervical cancer patients at dr hasan sadikin general hospital |
| topic | MTHFR C677T polymorphism Bandung Cervical cancer Bandung |
| url | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/754 |
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