Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement
Abstract Ca3SiO5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibrob...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
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| Series: | Brazilian Oral Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100277&lng=en&tlng=en |
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| author | Gisele Alborghetti NAI Gustavo de Almeida LOGAR Graziela Garrido MORI Ligia Moraes TEIXEIRA Bruna Camila Ferreira da SILVA Ana Elisa Maranho de MORAES Felipe André CABRAL |
| author_facet | Gisele Alborghetti NAI Gustavo de Almeida LOGAR Graziela Garrido MORI Ligia Moraes TEIXEIRA Bruna Camila Ferreira da SILVA Ana Elisa Maranho de MORAES Felipe André CABRAL |
| author_sort | Gisele Alborghetti NAI |
| collection | DOAJ |
| description | Abstract Ca3SiO5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca3SiO5in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca3SiO5-based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca3SiO5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca3SiO5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca3SiO5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca3SiO5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca3SiO5-based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay. |
| first_indexed | 2024-04-14T04:35:40Z |
| format | Article |
| id | doaj.art-fbe246cc0bb44634933bf43bb88f5880 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-04-14T04:35:40Z |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-fbe246cc0bb44634933bf43bb88f58802022-12-22T02:11:54ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-310730110.1590/1807-3107BOR-2016.vol30.0097S1806-83242016000100277Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cementGisele Alborghetti NAIGustavo de Almeida LOGARGraziela Garrido MORILigia Moraes TEIXEIRABruna Camila Ferreira da SILVAAna Elisa Maranho de MORAESFelipe André CABRALAbstract Ca3SiO5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca3SiO5in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca3SiO5-based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca3SiO5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca3SiO5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca3SiO5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca3SiO5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca3SiO5-based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100277&lng=en&tlng=ensilicate cementgenotoxicitymutagenicitybiomaterial |
| spellingShingle | Gisele Alborghetti NAI Gustavo de Almeida LOGAR Graziela Garrido MORI Ligia Moraes TEIXEIRA Bruna Camila Ferreira da SILVA Ana Elisa Maranho de MORAES Felipe André CABRAL Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement Brazilian Oral Research silicate cement genotoxicity mutagenicity biomaterial |
| title | Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement |
| title_full | Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement |
| title_fullStr | Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement |
| title_full_unstemmed | Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement |
| title_short | Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement |
| title_sort | evaluation of the genotoxicity and mutagenicity of ca3sio5 based cement |
| topic | silicate cement genotoxicity mutagenicity biomaterial |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100277&lng=en&tlng=en |
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