How Adding Chlorhexidine or Metallic Nanoparticles Affects the Antimicrobial Performance of Calcium Hydroxide Paste as an Intracanal Medication: An In Vitro Study
The aim of our study was to explore the potential value of metallic (Ag, Cu, and Zn) salts, polymer/metallic nanoparticles, and chlorhexidine (CHX) for improving the antimicrobial activity of calcium hydroxide (CH) against <i>E. faecalis</i> and <i>C. albicans</i>, associated...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/11/1352 |
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| author | Kadiatou Sy Kevimy Agossa Mickaël Maton Henry Chijcheapaza-Flores Bernard Martel Florence Siepmann Etienne Deveaux Nicolas Blanchemain Christel Neut |
| author_facet | Kadiatou Sy Kevimy Agossa Mickaël Maton Henry Chijcheapaza-Flores Bernard Martel Florence Siepmann Etienne Deveaux Nicolas Blanchemain Christel Neut |
| author_sort | Kadiatou Sy |
| collection | DOAJ |
| description | The aim of our study was to explore the potential value of metallic (Ag, Cu, and Zn) salts, polymer/metallic nanoparticles, and chlorhexidine (CHX) for improving the antimicrobial activity of calcium hydroxide (CH) against <i>E. faecalis</i> and <i>C. albicans</i>, associated with persistent endodontic infections. A first screening was performed by determining minimum inhibitory/bactericidal concentrations (MIC/MBC). Antimicrobial activity of the CH paste mixed with metallic salts, chitosan or cyclodextrin polymer metallic nanoparticles was compared to the antimicrobial activity of CH paste alone and CH + CHX using a time-kill kinetics assay. The effect of the antimicrobials on the rheological and the key mechanical properties were also examined. Copper and zinc were discarded because of their MIC/MBC values and silver because of its kill time curve profile. Except for a slower setting time after 24 h and a higher weight loss after 1 week of incubation, the mechanical behavior of the CH paste was unaffected by the addition of CHX. Polymeric/metallic nanoparticles failed to potentiate the antimicrobial effect of CH. By contrast, CHX increased this effect and thus could help eradicate <i>E. faecalis</i> associated with persistent root canal infections without altering the desired key physical properties of the CH paste. |
| first_indexed | 2024-03-10T05:46:42Z |
| format | Article |
| id | doaj.art-fbe37b1bede249f1842d00f6d823ba69 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T05:46:42Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-fbe37b1bede249f1842d00f6d823ba692023-11-22T22:10:20ZengMDPI AGAntibiotics2079-63822021-11-011011135210.3390/antibiotics10111352How Adding Chlorhexidine or Metallic Nanoparticles Affects the Antimicrobial Performance of Calcium Hydroxide Paste as an Intracanal Medication: An In Vitro StudyKadiatou Sy0Kevimy Agossa1Mickaël Maton2Henry Chijcheapaza-Flores3Bernard Martel4Florence Siepmann5Etienne Deveaux6Nicolas Blanchemain7Christel Neut8U1008—Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, University of Lille, 59000 Lille, FranceU1008—Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, University of Lille, 59000 Lille, FranceU1008—Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, University of Lille, 59000 Lille, FranceU1008—Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, University of Lille, 59000 Lille, FranceUMR 8207, UMET—Unité Matériaux et Transformations, CNRS—Centre National de la Recherche Scientifique, INRA—Institut National de la Recherche Agronomique, ENSCL—Ecole Nationale Supérieure de Chimie de Lille, University of Lille, 59655 Lille, FranceU1008—Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, University of Lille, 59000 Lille, FranceU1008—Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, University of Lille, 59000 Lille, FranceU1008—Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, University of Lille, 59000 Lille, FranceU1286 Infinite—Institute for Translational Research in Inflammation, Inserm, CHU Lille, University of Lille, 59000 Lille, FranceThe aim of our study was to explore the potential value of metallic (Ag, Cu, and Zn) salts, polymer/metallic nanoparticles, and chlorhexidine (CHX) for improving the antimicrobial activity of calcium hydroxide (CH) against <i>E. faecalis</i> and <i>C. albicans</i>, associated with persistent endodontic infections. A first screening was performed by determining minimum inhibitory/bactericidal concentrations (MIC/MBC). Antimicrobial activity of the CH paste mixed with metallic salts, chitosan or cyclodextrin polymer metallic nanoparticles was compared to the antimicrobial activity of CH paste alone and CH + CHX using a time-kill kinetics assay. The effect of the antimicrobials on the rheological and the key mechanical properties were also examined. Copper and zinc were discarded because of their MIC/MBC values and silver because of its kill time curve profile. Except for a slower setting time after 24 h and a higher weight loss after 1 week of incubation, the mechanical behavior of the CH paste was unaffected by the addition of CHX. Polymeric/metallic nanoparticles failed to potentiate the antimicrobial effect of CH. By contrast, CHX increased this effect and thus could help eradicate <i>E. faecalis</i> associated with persistent root canal infections without altering the desired key physical properties of the CH paste.https://www.mdpi.com/2079-6382/10/11/1352endodontic infectionscalcium hydroxidechlorhexidinecoppersilverzinc |
| spellingShingle | Kadiatou Sy Kevimy Agossa Mickaël Maton Henry Chijcheapaza-Flores Bernard Martel Florence Siepmann Etienne Deveaux Nicolas Blanchemain Christel Neut How Adding Chlorhexidine or Metallic Nanoparticles Affects the Antimicrobial Performance of Calcium Hydroxide Paste as an Intracanal Medication: An In Vitro Study Antibiotics endodontic infections calcium hydroxide chlorhexidine copper silver zinc |
| title | How Adding Chlorhexidine or Metallic Nanoparticles Affects the Antimicrobial Performance of Calcium Hydroxide Paste as an Intracanal Medication: An In Vitro Study |
| title_full | How Adding Chlorhexidine or Metallic Nanoparticles Affects the Antimicrobial Performance of Calcium Hydroxide Paste as an Intracanal Medication: An In Vitro Study |
| title_fullStr | How Adding Chlorhexidine or Metallic Nanoparticles Affects the Antimicrobial Performance of Calcium Hydroxide Paste as an Intracanal Medication: An In Vitro Study |
| title_full_unstemmed | How Adding Chlorhexidine or Metallic Nanoparticles Affects the Antimicrobial Performance of Calcium Hydroxide Paste as an Intracanal Medication: An In Vitro Study |
| title_short | How Adding Chlorhexidine or Metallic Nanoparticles Affects the Antimicrobial Performance of Calcium Hydroxide Paste as an Intracanal Medication: An In Vitro Study |
| title_sort | how adding chlorhexidine or metallic nanoparticles affects the antimicrobial performance of calcium hydroxide paste as an intracanal medication an in vitro study |
| topic | endodontic infections calcium hydroxide chlorhexidine copper silver zinc |
| url | https://www.mdpi.com/2079-6382/10/11/1352 |
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