Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history
IntroductionInternational travel has been a major determinant for the introduction of pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) into naïve geographic areas. MRSA clonal complex 239 (CC239) is a highly virulent clone that is predominant in Asia. The objective of this study...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-11-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016829/full |
| _version_ | 1811217610221551616 |
|---|---|
| author | Jasmine Coppens Basil Britto Xavier Jelle Vlaeminck Jesper Larsen Christine Lammens Sandra Van Puyvelde Sandra Van Puyvelde Herman Goossens Anders Rhod Larsen Surbhi Malhotra-Kumar |
| author_facet | Jasmine Coppens Basil Britto Xavier Jelle Vlaeminck Jesper Larsen Christine Lammens Sandra Van Puyvelde Sandra Van Puyvelde Herman Goossens Anders Rhod Larsen Surbhi Malhotra-Kumar |
| author_sort | Jasmine Coppens |
| collection | DOAJ |
| description | IntroductionInternational travel has been a major determinant for the introduction of pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) into naïve geographic areas. MRSA clonal complex 239 (CC239) is a highly virulent clone that is predominant in Asia. The objective of this study was to determine the geographic origin of MRSA CC239 isolates recovered from Danish cases with or without a history of international travel during 2004–2016.Materials and methodsHuman MRSA isolates with spa types t030 and t037 (n = 60) were obtained from the National Reference Laboratory for Antimicrobial Resistance. For each case, the following data were collected from notification forms: sex, age, isolation year, specimen source (screening swab or clinical sample), infection type, and international travel history. All isolates were whole-genome sequenced, and a comparative genome and phylogenetic analysis was performed.ResultsThe majority of isolates originated from skin and soft tissue (SST) infections and screening swabs. In 31 out of 60 cases reported international travel to different parts of the world. Fifty-four isolates belonged to CC239, including sequence type 239 (ST239) (n = 43), ST241 (n = 5), ST4377 (n = 2), ST4378 (n = 1), ST1465 (n = 1), ST343 (n = 1), and ST592 (n = 1). The majority of the CC239 MRSA isolates (40/54) belonged to well-known geographic clades, including the Asian (n = 12), Serbian (n = 11), South American (n = 2), and Turkish (n = 15). Most MRSA ST239 isolates belonging to the highly virulent Asian clade carried sasX and were recovered from individuals who had travelled to Asia, Africa and the Middle East.ConclusionOur data reveal multiple introductions of MRSA CC239 into Denmark through international travel, which highlights the importance of continued genomic surveillance of MRSA in persons returning from international travel to areas where MRSA is endemic. |
| first_indexed | 2024-04-12T06:57:42Z |
| format | Article |
| id | doaj.art-fbe68c6d5af54ee499d26c1afbaebb81 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T06:57:42Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fbe68c6d5af54ee499d26c1afbaebb812022-12-22T03:43:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10168291016829Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel historyJasmine Coppens0Basil Britto Xavier1Jelle Vlaeminck2Jesper Larsen3Christine Lammens4Sandra Van Puyvelde5Sandra Van Puyvelde6Herman Goossens7Anders Rhod Larsen8Surbhi Malhotra-Kumar9Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumLaboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumLaboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumDepartment of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, DenmarkLaboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumLaboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumCambridge Institute of Therapeutic Immunology and Infectious Disease, University of Cambridge School of Clinical Medicine, Cambridge Biomedical Campus, Cambridge, United KingdomLaboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumDepartment of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, DenmarkLaboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, BelgiumIntroductionInternational travel has been a major determinant for the introduction of pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) into naïve geographic areas. MRSA clonal complex 239 (CC239) is a highly virulent clone that is predominant in Asia. The objective of this study was to determine the geographic origin of MRSA CC239 isolates recovered from Danish cases with or without a history of international travel during 2004–2016.Materials and methodsHuman MRSA isolates with spa types t030 and t037 (n = 60) were obtained from the National Reference Laboratory for Antimicrobial Resistance. For each case, the following data were collected from notification forms: sex, age, isolation year, specimen source (screening swab or clinical sample), infection type, and international travel history. All isolates were whole-genome sequenced, and a comparative genome and phylogenetic analysis was performed.ResultsThe majority of isolates originated from skin and soft tissue (SST) infections and screening swabs. In 31 out of 60 cases reported international travel to different parts of the world. Fifty-four isolates belonged to CC239, including sequence type 239 (ST239) (n = 43), ST241 (n = 5), ST4377 (n = 2), ST4378 (n = 1), ST1465 (n = 1), ST343 (n = 1), and ST592 (n = 1). The majority of the CC239 MRSA isolates (40/54) belonged to well-known geographic clades, including the Asian (n = 12), Serbian (n = 11), South American (n = 2), and Turkish (n = 15). Most MRSA ST239 isolates belonging to the highly virulent Asian clade carried sasX and were recovered from individuals who had travelled to Asia, Africa and the Middle East.ConclusionOur data reveal multiple introductions of MRSA CC239 into Denmark through international travel, which highlights the importance of continued genomic surveillance of MRSA in persons returning from international travel to areas where MRSA is endemic.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016829/fullMRSACC239ST239sasXDenmarkWGS |
| spellingShingle | Jasmine Coppens Basil Britto Xavier Jelle Vlaeminck Jesper Larsen Christine Lammens Sandra Van Puyvelde Sandra Van Puyvelde Herman Goossens Anders Rhod Larsen Surbhi Malhotra-Kumar Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history Frontiers in Microbiology MRSA CC239 ST239 sasX Denmark WGS |
| title | Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history |
| title_full | Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history |
| title_fullStr | Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history |
| title_full_unstemmed | Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history |
| title_short | Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history |
| title_sort | genomic analysis of methicillin resistant staphylococcus aureus clonal complex 239 isolated from danish patients with and without an international travel history |
| topic | MRSA CC239 ST239 sasX Denmark WGS |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016829/full |
| work_keys_str_mv | AT jasminecoppens genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT basilbrittoxavier genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT jellevlaeminck genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT jesperlarsen genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT christinelammens genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT sandravanpuyvelde genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT sandravanpuyvelde genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT hermangoossens genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT andersrhodlarsen genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory AT surbhimalhotrakumar genomicanalysisofmethicillinresistantstaphylococcusaureusclonalcomplex239isolatedfromdanishpatientswithandwithoutaninternationaltravelhistory |