Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1
Abstract Four bacterial isolates were obtained from marine sediments collected from Sahl Hashish, Hurghada Red Sea, Egypt. This study was designed to search for promising anti-Alzheimer natural polysaccharide; therefore, four isolates were screened for exopolysaccharides (EPSs) production and acetyl...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38172-z |
| _version_ | 1797778941059006464 |
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| author | Manal S. Selim Sahar S. Mohamed Mohsen S. Asker Abeer Y. Ibrahim Samah A. El-Newary Mohamed E. El Awady |
| author_facet | Manal S. Selim Sahar S. Mohamed Mohsen S. Asker Abeer Y. Ibrahim Samah A. El-Newary Mohamed E. El Awady |
| author_sort | Manal S. Selim |
| collection | DOAJ |
| description | Abstract Four bacterial isolates were obtained from marine sediments collected from Sahl Hashish, Hurghada Red Sea, Egypt. This study was designed to search for promising anti-Alzheimer natural polysaccharide; therefore, four isolates were screened for exopolysaccharides (EPSs) production and acetylcholinesterase inhibition. The isolate S16 provided the highest EPS yield (7.51 g/L) and acetylcholinesterase inhibition. It was identified morphologically and genetically using 16S rRNA gene sequence analysis as Bacillus maritimus. A Physicochemical analysis of S16 exopolysaccharide (BMEPS) was estimated, which pointed to the presence of uronic acid and sulfate (24.7% and 18.3%, respectively). HPLC analysis indicated that mannuronic acid, glucuronic acid, glucose, and mannose are presented in a molar ratio of 0.8:1.0:2.8:2.3, respectively. Furthermore, FT-IR revealed an abundance of β-configurations. The GPC estimated the average molecular weight (Mw) as 4.31 × 104 g/mol. BMEPS inhibited AChE (IC50; 691.77 ± 8.65 μg/ ml), BChE (IC50; 288.27 ± 10.50 μg/ ml), and tyrosinase (IC50; 3.34 ± 0.09, 14.00 ± 0.14, and 22.96 ± 1.23 μg/ ml during incubation durations of 10, 20, and 40 min). It also demonstrated a selective anti-inflammatory action against COX-2 rather than COX-1. Moreover, BMEPS exhibited antioxidant capabilities as free radical and oxygen reactive species (ROS) scavenger, metal chelator, reductant agent, and lipid peroxidation suppressor. These activities are due to the distinct chemical composition. The findings of this study indicate that BMEPS could be considered as promising anti-disease Alzheimer's (AD) material in an in-vitro model, which qualifies it for advanced in-vivo studies in the discovery of alternative Alzheimer’s treatment. |
| first_indexed | 2024-03-12T23:24:47Z |
| format | Article |
| id | doaj.art-fbe6ce58c28d4cca87dedb8f07b25a35 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T23:24:47Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fbe6ce58c28d4cca87dedb8f07b25a352023-07-16T11:16:07ZengNature PortfolioScientific Reports2045-23222023-07-0113111710.1038/s41598-023-38172-zCharacterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1Manal S. Selim0Sahar S. Mohamed1Mohsen S. Asker2Abeer Y. Ibrahim3Samah A. El-Newary4Mohamed E. El Awady5Microbial Biotechnology Department, Institute of Biotechnology Research, National Research CentreMicrobial Biotechnology Department, Institute of Biotechnology Research, National Research CentreMicrobial Biotechnology Department, Institute of Biotechnology Research, National Research CentreMedicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research Institute, National Research CentreMedicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research Institute, National Research CentreMicrobial Biotechnology Department, Institute of Biotechnology Research, National Research CentreAbstract Four bacterial isolates were obtained from marine sediments collected from Sahl Hashish, Hurghada Red Sea, Egypt. This study was designed to search for promising anti-Alzheimer natural polysaccharide; therefore, four isolates were screened for exopolysaccharides (EPSs) production and acetylcholinesterase inhibition. The isolate S16 provided the highest EPS yield (7.51 g/L) and acetylcholinesterase inhibition. It was identified morphologically and genetically using 16S rRNA gene sequence analysis as Bacillus maritimus. A Physicochemical analysis of S16 exopolysaccharide (BMEPS) was estimated, which pointed to the presence of uronic acid and sulfate (24.7% and 18.3%, respectively). HPLC analysis indicated that mannuronic acid, glucuronic acid, glucose, and mannose are presented in a molar ratio of 0.8:1.0:2.8:2.3, respectively. Furthermore, FT-IR revealed an abundance of β-configurations. The GPC estimated the average molecular weight (Mw) as 4.31 × 104 g/mol. BMEPS inhibited AChE (IC50; 691.77 ± 8.65 μg/ ml), BChE (IC50; 288.27 ± 10.50 μg/ ml), and tyrosinase (IC50; 3.34 ± 0.09, 14.00 ± 0.14, and 22.96 ± 1.23 μg/ ml during incubation durations of 10, 20, and 40 min). It also demonstrated a selective anti-inflammatory action against COX-2 rather than COX-1. Moreover, BMEPS exhibited antioxidant capabilities as free radical and oxygen reactive species (ROS) scavenger, metal chelator, reductant agent, and lipid peroxidation suppressor. These activities are due to the distinct chemical composition. The findings of this study indicate that BMEPS could be considered as promising anti-disease Alzheimer's (AD) material in an in-vitro model, which qualifies it for advanced in-vivo studies in the discovery of alternative Alzheimer’s treatment.https://doi.org/10.1038/s41598-023-38172-z |
| spellingShingle | Manal S. Selim Sahar S. Mohamed Mohsen S. Asker Abeer Y. Ibrahim Samah A. El-Newary Mohamed E. El Awady Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1 Scientific Reports |
| title | Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1 |
| title_full | Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1 |
| title_fullStr | Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1 |
| title_full_unstemmed | Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1 |
| title_short | Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1 |
| title_sort | characterization and in vitro alzheimer s properties of exopolysaccharide from bacillus maritimus msm1 |
| url | https://doi.org/10.1038/s41598-023-38172-z |
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